Method for isolating dnj and preparing dnj nanosuspension

Abstract

The invention discloses methods for separating DNJ (1-deoxynojirimycin) and preparing a DNJ nanometer suspension. The method for separating DNJ comprises: taking mulberry as a raw material, performing crushing extraction and microwave coking, and adding water to obtain an isolate I, using fine sand-active carbon and silica gel for twice chromatographic separation, so as to form an isolate II, performing ion exchange and ethanol precipitation on the isolate II to obtain an isolate III, performing aluminium oxide chromatographic column separation on the isolate III to obtain an isolate IV, and performing crystallization and recrystallization on the isolate IV to obtain the target product. The method for preparing the DNJ nanometer suspension comprises: firstly performing esterification modification on DNJ to enhance the lipid solubility of DNJ, and then performing surfactant combination and high-pressure homogenization processing to disperse DNJ to form a highly-dispersed particle swarm. The DNJ separation technology has the characteristics of being easily available in raw materials, simple in technology, obvious in effect and suitable for large-scale production, and the prepared DNJ nanometer suspension is capable of improving the solubility of a medicine and improving the bioavailability.

Description

technical field [0001] The invention belongs to the field of natural medicine chemistry, relates to medicine separation and purification technology, in particular to a method for large-scale separation and purification of DNJ from mulberry sources and preparation of DNJ component nano-suspension. Background technique [0002] Diabetes has become one of the main killers that endanger human health, and can cause chronic damage and dysfunction of the eyes, kidneys, heart, blood vessels, and nerves. It is estimated that by 2050, the number of people suffering from diabetes in the world will reach 300 million, of which 90% will be type 2 diabetes. At present, oral hypoglycemic agents for type 2 diabetes mainly include sulfonylureas, biguanides, α-glucosidase inhibitors and thiazolidinediones. Among them, α-glucosidase inhibitors have remarkable hypoglycemic effect and have received the most attention. Acarbose, voglibose, and miglitol, which are currently on the market, belong ...

AI Technical Summary

Problems solved by technology

[0005] At present, limited by the physical and chemical properties of DNJ itself, the existing separation technologies are either simple in operation and low in efficiency, or complex in technology and small in scale, which limits the practical and large-scale development of natural DNJ, resulting in the market investment of DNJ being far less than the actual demand. (relevant content can refer to the systematic summary and discussion made by the author on the advantages and disadvantages of various methods in the separation and purification research of plant DNJ at home and abroad in recent years (Summary of Plant DNJ Separation and Purification Technology, Sericulture Science, 2014, 40(3) :0544-0550))

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