Method for preparing DNJ nanosuspension

Abstract

The invention discloses a method for separating DNJ and a method for preparing a DNJ nanosuspension.According to the method for separating DNJ, mulberry leaves are used as a raw material and subjected to smashing extraction, microwave scorching and water dissolution, and then an outlier I is obtained; the outlier I is subjected to fine sand-activated carbon chromatographic separation and silica gel chromatographic separation in sequence to form an outlier II; the outlier II is subjected to ion exchange and ethanol precipitation to obtain an outlier III; the outlier III is subjected to aluminum oxide chromatographic column separation to obtain an outlier IV; the outlier IV is crystallized and recrystallized to obtain the target product.According to the method for preparing the DNJ nanosuspension, firstly, DNJ components are subjected to esterification modification so that the lipid solubility of the DNJ components can be enhanced, and then DNJ is dispersed into high-dispersity particle swarms by means of surfactant combination and high-pressure homogenization.The DNG separation technology has the advantages that raw materials are easy to obtain, the process is simple, the effect is obvious, and the technology is suitable for large-scale production.The prepared DNJ nanosuspension can improve the solubility of medicine and bioavailability.

Description

[0001] This application is a divisional application for the original application number: 201410614225.6, the original application date is October 31, 2014, and the title is "method for isolating DNJ and preparing DNJ nanosuspension". technical field [0002] The invention belongs to the field of natural medicine chemistry, relates to medicine separation and purification technology, in particular to a method for large-scale separation and purification of DNJ from mulberry sources and preparation of DNJ component nano-suspension. Background technique [0003] Diabetes has become one of the main killers that endanger human health, and can cause chronic damage and dysfunction of the eyes, kidneys, heart, blood vessels, and nerves. It is estimated that by 2050, the number of people suffering from diabetes in the world will reach 300 million, of which 90% will be type 2 diabetes. At present, oral hypoglycemic agents for type 2 diabetes mainly include sulfonylureas, biguanides, α-g...

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Problems solved by technology

[0006] At present, limited by the physical and chemical properties of DNJ itself, the existing separation technologies are either simple in operation and low in efficiency, or complex in technology and small in scale, which limits the practical and large-scale development of natural DNJ, resulting in the market investment of DNJ being far less than the actual demand. (relevant content can refer to the systematic summary and discussion made by the author on the advantages and disadvantages of various methods in the separation and purification research of plant DNJ at home and abroad in recent years (Summary of Plant DNJ Separation and Purification Technology, Sericulture Science, 2014, 40(3) :0544-0550))

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