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Porous biological material using modified gelatin as crosslinking agent and preparation method of porous biological material

A technology of biomaterials and porosity, applied in the field of biomedical materials

Inactive Publication Date: 2015-02-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no patent report on the preparation of porous biomaterials using modified gelatin as a crosslinking agent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Add 0.2 g of methacrylamide-modified gelatin with an amino substitution degree of 35% into a 20 mL stoppered glass bottle, then add 1.0 g of acrylamide monomer and 10 mL of deionized water, blow in nitrogen, and stir for 15 minutes until a homogeneous clear solution is formed. Then add 100 μL of N,N,N',N'-tetramethylethylenediamine solution with a concentration of 10% by mass, mix well and continue to pass nitrogen gas for 5 minutes, then add 200 μL of persulfuric acid with a concentration of 4% by mass Ammonium solution, then quickly sealed, placed in 20 o After reacting in C water bath for 12 hours, the reactants were taken out and soaked in 20 o C deionized water for 48 hours, changing the deionized water every 8 hours, and then freeze-drying for 24 hours to obtain a porous biomaterial with good biocompatibility.

Embodiment 2

[0028] Add 0.2 g of methacrylamide-modified gelatin with an amino substitution degree of 60 % and 0.2 g of inorganic nanoclay Laponite into a 20 mL stoppered glass bottle, then add 1.0 g of acrylamide monomer and 10 mL of deionized water , nitrogen gas was introduced, and stirred for 30 minutes until a uniform and transparent solution was formed. Then add 100 μL of N,N,N',N'-tetramethylethylenediamine solution with a concentration of 10% by mass, mix well and continue to pass nitrogen gas for 5 minutes, then add 200 μL of persulfuric acid with a concentration of 4% by mass ammonium solution, then quickly sealed and placed at -20 o After reacting in C refrigerator for 24 hours, the reactants were taken out and soaked in 20 o C deionized water for 48 hours, changing the deionized water every 8 hours, and then freeze-drying for 24 hours to obtain a porous biomaterial with good biocompatibility and rapid swelling.

Embodiment 3

[0030]Add 0.1 g of methacrylamide-modified gelatin with an amino substitution degree of 90% and 0.1 g of inorganic nanoclay Laponite into a 20 mL stoppered glass bottle, then add 1.0 g of N-isopropylacrylamide monomer and 10 mL of deionized water was blown with nitrogen, and stirred for 20 minutes until a uniform and transparent solution was formed. Then add 100 μL of N,N,N,N-tetramethylethylenediamine solution with a concentration of 10% by mass, mix well and continue to blow nitrogen gas for 5 minutes, then add 200 μL of ammonium persulfate solution with a concentration of 4% by mass , then quickly sealed and placed at -30 o After reacting in C refrigerator for 24 hours, the reactants were taken out and soaked in 20 o C deionized water for 48 hours, change the deionized water every 8 hours, and then freeze-dry for 24 hours to obtain a temperature-sensitive and fast-swelling porous biomaterial.

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Abstract

The invention provides a porous biological material using modified gelatin as a crosslinking agent and a preparation method of the porous biological material. The preparation method specially comprises the following steps: dissolving an acrylamide monomer, methacrylamide modified gelatin and inorganic nano-clay in deionized water, and stirring for 10 to 30 minutes in an inert atmosphere; adding a catalyst, stirring for 5 to 10 minutes in the inert atmosphere, and then adding an initiator; performing radical polymerization for 12 to 48 hours at the temperature of -30 DEG C to 30 DEG C; soaking the reaction product in the deionized water at 20 DEG C for 48 to 72 hours, and replacing the deionized water every 5 to 8 hours, and finally performing freeze-drying on the reaction product for 24 to 48 hours to obtain the porous biological material using modified gelatin as the crosslinking agent. The preparation method is simple in preparation process, the condition can be controlled, and the prepared porous biological material has the characteristics of good biocompatibility, mechanical property, macroporous property, quick swelling property and the like at the same time, and can be widely applied in the fields of biological scaffolds, cell culture, drug controlled release, tissue engineering, trauma and burn treatment and other biomedical materials.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a porous biomaterial using modified gelatin as a crosslinking agent and a preparation method thereof. Background technique [0002] Porous polymer materials have attracted the interest of a large number of researchers because of their extremely high porosity and good swelling properties. Porous materials have different applications in the fields of biomedicine, biotechnology and pharmaceuticals, especially in tissue engineering as cell scaffold materials. Good porosity and pore size can provide more space and better fluidity for cell adhesion, growth and reproduction. Porous materials can usually be prepared by techniques such as freeze-drying, porogen method, microemulsion method, gas agitation and phase separation. Acrylamide monomers are used to prepare porous materials because of their good hydrophilicity and reactivity. Fatima et al prepared a macroporous porous materi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F290/00C08F220/56C08F220/54C08F2/44C08K3/34C08J9/28
Inventor 林炜李昌朋穆畅道王春华
Owner SICHUAN UNIV
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