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Hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters

A technology of gold nanoclusters and cysteine, which is applied in the field of hydrogen peroxide determination, can solve the problems of high biological toxicity, poor photostability, and high cost, and achieve the effect of fast preparation process, high sensitivity, and short analysis time

Inactive Publication Date: 2015-02-04
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the fluorescence method for the determination of H 2 o 2 Some small molecule organic dyes are mainly used as probes, which have poor photostability, high biological toxicity, prone to autoxidation, and high cost, which largely limits their practical application.

Method used

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  • Hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters
  • Hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters
  • Hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0034] Add 0.6 mL of 0.5 mol / L sodium hydroxide solution and 0.4 mL of 0.02 g / L chloroauric acid solution to 4 mL of 0.08 mol / L N-acetyl-L-cysteine ​​solution , mixed well, and placed in a 37°C constant temperature water bath to react for 2.5 h. After the reaction, the reaction solution was purified with a dialysis bag with a molecular weight cut off of 3500. The obtained gold nanocluster solution is colorless under visible light, and produces strong red fluorescence under ultraviolet light irradiation. Stored in the dark at 4°C, it can remain relatively stable for at least one month.

example 2

[0036] Get 0.2 ml of the gold nanocluster solution prepared in Example 1 and add to 0.2 ml concentration of 25 μmol / L H 2 o 2 solution (pH=3), after mixing evenly, add 0.05 ml of Fe with a concentration of 0.9 mmol / L 2+ , shake well and place in a 25°C water bath for 10 minutes. Observed under ultraviolet light, the gold nanocluster solution itself exhibits red fluorescence ( figure 1 In A), the red fluorescence of the gold nanocluster solution is quenched after the Fenton reaction ( figure 1 in B). figure 2 is the gold nanocluster solution ( figure 2 A) in A) and the gold nanocluster solution after the Fenton reaction ( figure 2 In B) Fluorescence emission spectrum graph.

example 3

[0038] Get 0.2 ml of the gold nanocluster solution prepared in Example 1 and add to 0.2 ml concentration of 100 μmol / L H 2 o 2 solution (pH=3), after mixing evenly, add 0.05 ml of Fe with a concentration of 0.9 mmol / L 2+ , shake well and place in a 25°C water bath for 10 minutes. The solution after the reaction is freeze-dried, and the dried powder is taken for X-ray photoelectron spectroscopy analysis. Such as image 3 As shown, the 4f of gold after the Fenton reaction 7 / 2 The binding energy shifted from the original 84.57 eV to 84.75 eV, indicating that the hydroxyl radicals generated by the Fenton reaction oxidized the gold atoms, which led to the quenching of the fluorescence of the gold nanoclusters.

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Abstract

The invention discloses a hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters, and relates to a hydrogen peroxide measurement method which takes the gold nanoclusters protected by N-acetyl-L-cysteine as a fluorescent probe. The method is characterized in that the fluorescent light of the gold nanoclusters is quenched by utilizing hydroxyl radicals generated by catalyzing H2O2 through Fe<2+>, so that the characteristic of a fluorescence emission spectrum is changed, and the method can be used for detecting H2O2. Delta F650 and the concentration of H2O2 have a linear relation within the range of 0.044-6.66 micromole / L, and the limit of detection is 27 nmol / L. The method is high in sensitivity and good in reproducibility, and can be used for measuring H2O2 in food, industry, environment and a living system.

Description

technical field [0001] The invention relates to a method for measuring hydrogen peroxide using gold nanoclusters protected by N-acetyl-L-cysteine ​​as fluorescent probes, and belongs to the fields of analytical chemistry and nanotechnology. Background technique [0002] hydrogen peroxide (H 2 o 2 ) is a very important chemical reagent, which can be used as a disinfectant, bactericide, deoxidizer, bleach and polymerization initiator, and it is also an important chemical product in textile, chemical, environmental protection, electronics, food There are a wide range of applications in the health industry and other fields. In addition, H 2 o 2 It plays an important role in living organisms, it is an essential substance in the process of cell activity, and has been proven to be the second messenger of cell signal transmission. In addition, H 2 o 2 It is also the main product of many biological oxidases (such as glucose oxidase, cholesterol oxidase, lactate oxidase, cholin...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 陈伟邓豪华何栋林静彭花萍
Owner FUJIAN MEDICAL UNIV
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