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A kind of traditional Chinese medicine composition and identification method thereof

A technology of composition and traditional Chinese medicine, which is applied in the direction of drug combination, medical formula, plant raw materials, etc., can solve the problems such as the identification method of drug ingredients is not given, and achieve the effect of improving safety and reliability and strong specificity

Active Publication Date: 2020-03-20
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the traditional Chinese medicine composition capsule, only the medicinal ingredients and preparation method are disclosed at present, and no identification method for the corresponding medicinal ingredients is given

Method used

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  • A kind of traditional Chinese medicine composition and identification method thereof
  • A kind of traditional Chinese medicine composition and identification method thereof
  • A kind of traditional Chinese medicine composition and identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The proportioning of this Chinese medicine composition is as follows:

[0049]Astragalus 35g, Ligustrum lucidum 9g, talc 9g, Prunella vulgaris 15g, litchi core 15g, amber 1g, cinnamon 2g, Phellodendron 5g.

[0050] The preparation process of this Chinese medicine composition capsule is:

[0051] (1) Take the above-mentioned Chinese medicinal materials, select them separately, crush them, and weigh them according to the prescription; (2) Take Astragalus membranaceus and Cortex Phellodendri, add 6 times the amount of 50% ethanol, heat and reflux for extraction once, extract for 0.5 hours, and filter the extract , combined, after recovering ethanol under reduced pressure, concentrate it into a clear paste with a relative density of 1.20, put it into a vacuum drying oven, and dry under reduced pressure at a temperature of 60°C and a vacuum degree of 0.04Mpa, and the dry paste is ready for use;

[0052] (3) Take Ligustrum lucidum, add 6 times the amount of 70% ethanol, heat...

Embodiment 2

[0064] The preparation of the prescription and capsules is the same as in Example 1.

[0065] The identification method of Ligustrum lucidum in the Chinese medicine composition consists of the following steps:

[0066] a, get the Chinese medicine composition capsule equivalent to 3g of crude drug amount, about 0.7g, pour out the contents, grind finely, add methanol 10ml, sonicate for 10 minutes, filter, and the filtrate is used as the test solution;

[0067] b. Take another oleanolic acid reference substance, add methanol to make a solution containing 0.5mg per 1ml, as the reference substance solution;

[0068] c. According to the thin-layer chromatography test, draw 2 μl of the test solution and 2 μl of the reference solution, and place them on the same silica gel G thin-layer plate with sodium carboxymethylcellulose as the binder, at 20:5:8: 0.5 cyclohexane-chloroform-ethyl acetate-glacial acetic acid as developing agent, develop, take out, dry, spray with 10% sulfuric acid...

Embodiment 3

[0070] The preparation of the prescription and capsules is the same as in Example 1.

[0071] The identification method of Cortex Phellodendri in the Chinese medicine composition consists of the following steps:

[0072] a, get the Chinese medicine composition capsule equivalent to 3g of crude drug amount, about 0.7g, pour out the contents, grind finely, add methanol 10ml, sonicate for 10 minutes, filter, and the filtrate is used as the test solution;

[0073] b. Take another 0.1 g of Cortex Phellodendri as reference medicinal material, add 5 ml of methanol, ultrasonically treat for 10 minutes, filter, and use the filtrate as the reference medicinal material solution;

[0074] c. According to the thin-layer chromatography test, draw 2 μl of the test solution and 2 μl of the reference medicinal material solution, and place them on the same silica gel G thin-layer plate with sodium carboxymethylcellulose as the binder, at a ratio of 10:6:1: 1 Ethyl acetate-butanone-formic acid-...

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Abstract

The invention discloses a traditional Chinese medicine composition and an identification method thereof, which belong to the field of traditional Chinese medicines. In the quality control of drugs, improve the safety and reliability of drugs.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a traditional Chinese medicine composition and an identification method thereof. Background technique [0002] Traditional Chinese medicine has been used to treat diseases for thousands of years. With the development of science and technology, Chinese medicine is also facing modernization. To enter the world market, it is necessary to do a good job in quality control of Chinese medicine. The quality control of Chinese medicine components is the most important. Crucially, especially for compound Chinese medicine preparations, in order to fully control the quality of Chinese medicine, the identification of each component must be done well. [0003] The traditional Chinese medicine composition is composed of astragalus, privet lucidum, talc, prunella vulgaris, lychee core, amber, cinnamon and cortex cortex, and adopts the principles of invigorating the spleen ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/77A61K9/48G01N30/90A61P13/08A61K33/12A61K35/10
CPCA61K9/20A61K9/48A61K33/12A61K35/10A61K36/481A61K36/536A61K36/54A61K36/638A61K36/756A61K36/77A61K2236/13A61K2236/15A61K2236/331A61K2236/333A61K2236/39A61K2236/51A61K2236/53G01N30/90A61K2300/00
Inventor 刘敏彦董超秦拢
Owner HEBEI YILING MEDICINE INST
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