Preparation method of lyceum barbarum polysaccharide

Abstract

The invention relates to a preparation method of a plant polysaccharide, belonging to the field of food additives and preparation methods thereof. A preparation method of a lyceum barbarum polysaccharide comprises the following steps of (1) screening lyceum barbarum, removing impurities in the lyceum barbarum, then, dehydrating through hot air drying, crushing by using a coarse crusher, and then, separating pulp powder and lyceum barbarum seeds by using a three-stage sieve; (2) sieving the pulp powder by using a 20-mesh sieve, then, charging, and carrying out supercritical CO2 extraction; (3) adding pure water into an extraction tank, extracting by heating, adding pectinase and cellulase when cooling to 45 DEG C at the later period of extraction, preserving the heat, and carrying out centrifugal separation; and (4) desalting the separated juice through electroosmosis, then, carrying out four-stage ultrafiltration treatment and reverse-osmosis concentration, then, freezing, carrying out sublimation drying, crushing at low temperature, and packaging to obtain a finished product of the lyceum barbarum polysaccharide. The preparation method of the lyceum barbarum polysaccharide, provided by the invention, is simple in process, strong in operability and suitable for industrial production; the recovery rate is high; and the quality of the lyceum barbarum polysaccharide is stable.

Description

technical field [0001] The invention relates to a preparation method of plant polysaccharides, belonging to the field of food additives and preparation methods thereof. Background technique [0002] Lycium barbarum polysaccharide (LBP) is one of the active components of Lycium barbarum, and it is a rare protein-containing polysaccharide among plant polysaccharides. Lycium barbarum polysaccharides isolated from wolfberry have polysaccharide side chains composed of six hexoses (rhamnose, arabinose, xylose, mannose, galactose, glucose) and protein backbones composed of 18 amino acids. Glycml-O-Der link, this glycoprotein has biological activity, is a high-strength immune enhancer. [0003] Lycium barbarum polysaccharide is generally used as a raw material of health food, which can improve human immunity, enhance hematopoietic function, protect liver and lower blood pressure, and enhance cell activity. It is widely used in medicine, health food and other fields. [0004] The t...

AI Technical Summary

Problems solved by technology

Using this method to extract Lycium barbarum polysaccharides, the product purity is low, due to long-time heating, the product is easy to brown, the polysaccharide yield is low and the product quality is unstable, so it is rarely used now

[0005] At present, there are also crude polysaccharides prepared by ethanol precipitation, and then purified by column chromatography. Due to the complexity of the column chromatography process, it is in the stage of laboratory exploration and research.