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Insoluble polysaccharide compound with hemostatic function and preparation method thereof

An insoluble and complex technology, applied in the field of biomedical materials, can solve the problems of high activity, low thrombin adsorption, limited application, etc., and achieve the effect of high activity and high coagulation efficiency

Active Publication Date: 2014-12-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the interaction between chitosan surface groups and thrombin, the activity of thrombin decreased
In addition, due to the weak interaction between chitosan and thrombin, the adsorption of thrombin is not high.
At the same time, due to the high price of thrombin (the price of bovine thrombin is about 30,000 yuan / gram), it greatly limits its application on the surface of chitosan as hemostasis
[0004] In summary, chitosan-loaded thrombin hemostatic materials have the following defects: 1) thrombin is expensive (the price of bovine thrombin is about 30,000 yuan / g); 2) the method of loading thrombin on the surface of chitosan materials, The interaction between the two is weak, and the effective combination of thrombin and hemostatic materials cannot be realized. The loaded thrombin is easy to fall off. When used for wound hemostasis, the fallen thrombin may enter the blood vessel and cause thrombus; 3) Thrombin is loaded to the shell There is a large loss of activity behind the surface of the glycan, so the product is difficult to achieve commercialization
[0005] Compared with chitosan materials, cellulose and starch materials have better biocompatibility, weaker activation of platelets, and relatively poorer hemostatic effect
At present, the hemostatic agent for cellulose is mainly to modify cellulose or load thrombin. Due to the high price of loaded thrombin and the loss of thrombin activity during the loading process, it is difficult to commercialize
The hemostatic agent for starch mainly uses the strong water absorption of starch and loads thrombin, so it also faces the problems of loss of thrombin activity and high price

Method used

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  • Insoluble polysaccharide compound with hemostatic function and preparation method thereof
  • Insoluble polysaccharide compound with hemostatic function and preparation method thereof
  • Insoluble polysaccharide compound with hemostatic function and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0082] Preparation of chitosan / plasma complex: mix 50mg chitosan material (molecular weight 150,000-200,000) with 100ul anticoagulated plasma (according to the mass ratio of 1:2), add calcium ion to make the concentration 5mM, 25 °C for half an hour to obtain a chitosan / plasma composite material, which was vacuum-dried at 30°C for 5 hours to obtain a chitosan / plasma composite.

[0083] Determination of the coagulation effect of the chitosan / plasma complex: grind the chitosan / plasma complex, and conduct an in vitro coagulation experiment at 25°C, and keep the whole blood in a water bath at 25°C for half an hour in advance. Take 50mg chitosan / plasma complex, then add 40ul of 0.2M CaCl 2 , finally add 2ml of constant temperature whole blood, investigate its coagulation effect, the time when the whole blood coagulates is recorded as the coagulation time of chitosan / plasma complex. Repeat the above-mentioned steps of measuring blood coagulation time three times, get the average va...

Embodiment 2

[0086] Preparation of chitosan / whole blood complex: mix 50mg chitosan (molecular weight 150,000-200,000) with 100ul whole blood (according to the mass ratio of 1:2), add calcium ions to make the concentration 5mM, 25℃ Mix for half an hour to obtain a chitosan / whole blood composite material, which is vacuum-dried at 30° C. for 5 hours to obtain a chitosan / whole blood composite.

[0087] Determination of the coagulation effect of the chitosan / whole blood complex: Grind the chitosan / whole blood complex, and conduct an in vitro coagulation experiment at 25°C, and place the whole blood in a water bath at 25°C for half an hour in advance. Take 50mg chitosan / whole blood complex, then add 40ul of 0.2M CaCl 2 , finally add 2ml of constant temperature whole blood to investigate its coagulation effect, and the time when the whole blood coagulates is recorded as the coagulation time of the chitosan / whole blood complex. Repeat the above-mentioned steps of measuring coagulation time three ...

Embodiment 3

[0097] Preparation of different ratios of chitosan / plasma complexes: Mix 50mg chitosan (molecular weight 150,000-200,000) with 25ul, 50ul, 100ul, 200ul, 400ul anticoagulated plasma (1:0.5, 1:0, respectively) 1, 1:2, 1:4, 1:8 mass ratio), add calcium ions to make the concentration 5mM, and mix for half an hour at 25°C to prepare chitosan / 25ul plasma composite material and chitosan / 50ul plasma composite material, chitosan / 100ul plasma composite material, chitosan / 200ul plasma composite material, chitosan / 400ul plasma composite material were vacuum-dried at 30°C to obtain chitosan respectively / 25ul plasma complex, chitosan / 50ul plasma complex, chitosan / 100ul plasma complex, chitosan / 200ul plasma complex, chitosan / 400ul plasma complex.

[0098] Determination of coagulation effect of chitosan / plasma complexes with different ratios: In vitro coagulation experiment was carried out at 25°C, and the whole blood was kept in a water bath at 25°C for half an hour in advance. Take 50mg ...

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Abstract

The invention provides an insoluble polysaccharide compound with a hemostatic function. The compound includes insoluble polysaccharide and a blood component. The blood component is whole blood or plasma, and the insoluble polysaccharide is macromolecular polysaccharide insoluble in whole blood or plasma. According to the invention, by mixing the blood component with the insoluble polysaccharide, the blood component undergoes clotting reaction evenly and dispersedly on the surface of the insoluble polysaccharide, so that clotting factors generated in the clotting process can be adsorbed by the insoluble polysaccharide surface, and high clotting activity can be maintained, thereby obtaining a composite hemostatic material with an excellent clotting effect. The preparation method of the insoluble polysaccharide compound provided by the invention is simple, and is convenient and safe to use, thus being widely applicable to trauma and surgical hemostasis. The insoluble polysaccharide compound can be combined with other medical materials and medical instruments so as to be prepared into band aids, infusion bands, self-adhesive dressings, tablets and capsule core materials, operation packages and first-aid packets, etc. At the same time, the adsorption performance and other characteristics of the insoluble polysaccharide compound are utilized to adsorb antibiotics, painkillers and the like thereinto, thus achieving better curative effect.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to an insoluble polysaccharide compound with hemostatic function, a preparation method thereof, and a hemostatic material containing the compound. Background technique [0002] Hemostasis is a critical step in emergency lifesaving. The development of hemostatic materials that can control emergency situations such as aortic bleeding in a short period of time (3 minutes) can win valuable time for further medical treatment and greatly reduce deaths caused by bleeding and trauma. [0003] At present, there have been reports on the application of insoluble polysaccharides to hemostasis, among which chitosan (CN202020720U, CN102526795A), cellulose (CN100348272C, CN1109756A) and starch (CN102178691B) are mainly used. Chitosan is a natural polycation polysaccharide obtained after deacetylation of chitin. Chitosan molecules have multiple functions such as coagulatio...

Claims

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Application Information

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IPC IPC(8): A61K35/14A61K35/16A61K9/06A61K9/70A61K9/16A61L15/40A61L15/28A61L15/44A61P7/04
Inventor 范杰李云龙廖晓峰尚小强陈昊
Owner ZHEJIANG UNIV
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