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New use of gentamycin

A gentamicin, application technology, applied in the new application field of gentamicin, can solve the problems of reducing cation influx, reducing cell excitability, etc.

Inactive Publication Date: 2014-10-08
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it has been reported in the literature that in Xenopus laevis oocytes and hippocampal cell systems cultured in vitro, gentamicin affects the neurotransmitter acetylcholine (Ach) and its receptors, reduces the extracellular calcium ion current, and blocks neuromuscular Adapter transmission; and can modulate a variety of cation channels, including cation-selective ligand-gated channels, calcium ion channels, potassium ion channels, reduce cation influx, thereby reducing cell excitability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Observation on Inhibition of Epilepsy Behavior in Rats by Intracerebroventricular Injection of Gentamicin

[0025] Establishment of rat epilepsy model: Place a 40×30×50cm 3 Transparent plexiglass box, before the drug injection, the rats were placed in the box 30 minutes in advance to adapt to the experimental environment. After being anesthetized by intraperitoneal injection of 9% chloral hydrate (300mg / kg), the rats were fixed on a stereotaxic apparatus (NS-2, Narishige, Japan), the hair on the top of the head was shaved and the scalp was disinfected, the skin was cut open and cauterized with 10% hydrogen peroxide. The subcutaneous tissue was burned to expose the Bregma point of the skull, and the injection site of the hippocampus CA3 area was determined on the skull according to the rat brain localization map (Paxinnos et al., 1998). For the meninges, KA (0.4ug / ul) was injected into the CA3 region of the hippocampus 4mm below the skull, and sutured. T...

Embodiment 2

[0033] Example 2: Observation of intraperitoneal injection of gentamicin on the inhibition of epileptic behavior in rats

[0034] Establishment of rat epilepsy model: Same as example one.

[0035] Drug treatment: Although the way of administering the drug into the lateral ventricle can directly reach the lesion area, the operation is complicated, which is not conducive to the implementation in the actual application process. Therefore, we then performed intraperitoneal injection of gentamicin and observed the corresponding behavioral response. It has been reported in the literature that gentamicin cannot pass through the blood-brain barrier, but since epilepsy is often accompanied by damage to the blood-brain barrier, intraperitoneal injection of gentamicin is feasible.

[0036] result: After intraperitoneal injection of gentamicin (0.6ml, 50mg / ml, n=9), it can inhibit the obvious seizure behavior of rats, and has a significant effect on the duration of III, IV, V seiz...

Embodiment 3

[0037] Example 3: EEG observation of gentamicin on the inhibition of epileptic behavior in rats

[0038] Rat electrode implantation: Place a 40×30×50cm 3Transparent plexiglass box, before the drug injection, the rats were placed in the box 30 minutes in advance to adapt to the experimental environment. After being anesthetized by intraperitoneal injection of 9% chloral hydrate (300mg / kg), the rats were fixed on a stereotaxic apparatus (NS-2, Narishige, Japan), the hair on the top of the head was shaved and the scalp was disinfected, the skin was cut open and cauterized with 10% hydrogen peroxide. The subcutaneous tissue was burned to expose the Bregma point of the skull, and the KA and lateral ventricle drug delivery base implantation points (AP-4.1mm, L4.2mm) were determined on the skull according to the rat brain localization map (Paxinnos et al., 1998), ( 0.3mm posterior to bregma, 1.3mm lateral to the midline). After the location was determined, a small hole was dril...

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Abstract

The invention relates to a new use of gentamycin. Gentamycin is injected to the paracele and the abdominal cavity of a rat, and the inhibition conditions of gentamycin on the attack frequency, grade, duration and epilepsy discharge of epilepsy of the rat are observed from the behavioristics and the electroencephalogram recordings to construct a novel inducible anti-epileptic medicine. The novel inducible anti-epileptic medicine established in the invention provides a research carrier for researching epilepsy and an anti-epileptic molecule mechanism, and also provides a screening platform for researching and developing novel anti-epileptic medicines.

Description

technical field [0001] The invention relates to a new application of gentamicin. Background technique [0002] Epilepsy is a common chronic central system disease with an incidence rate of 0.5-1%. The etiology is complex and can be caused by genetic factors, traumatic brain injury, stroke, tumor and other reasons. It not only causes physical harm to patients, but also seriously affects It can lead to a series of mental and psychological problems and bring a heavy burden to the family and society. Factors such as seizures, cognitive function, mental behavioral state, social function, self-confidence and shame may affect the quality of life of patients. Its main feature is the abnormal synchronous discharge of neurons in the central nervous system or the whole brain area, resulting in muscle twitching and (or) loss of consciousness in behavior. Sensory, behavioral, and autonomic disturbances. As a kind of nervous excitatory disease, there are many mechanisms of epileptic se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7036A61P25/08
Inventor 朱红艳姜南赵玉晓王琴王子逸
Owner SHANGHAI UNIV
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