Method for in-situ detecting Hg<2+> in water sample through photocatalysis visible manner

An on-site detection and photocatalysis technology is applied in the direction of analyzing materials through chemical reactions and analyzing materials through observation of the influence of chemical indicators. It can solve problems such as low sensitivity and excessive Hg2+ content, and achieve high sensitivity. , the effect of simple method

Inactive Publication Date: 2014-08-13
CHENGDU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Although these methods are simple, fast and highly selective, they are not sensitive enough to be directly used in the field / field analysis of Hg in water samples. 2+ Whether the content exceeds the standard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Sequentially add 10 μL to a clean culture plate at a concentration of 10 μmol L -1 DNA solution (sequence 5'-CGCATTCAGGATTCTCTACTCGTA-3', 5'-TTCGTGTTGTGTTTCCTGTTTGCG-3'), 1mL of different concentrations of Hg 2+ Standard solution / environmental water sample, 100 μL concentration is 2 mmol L -1 Ethidium bromide solution (final concentration 100 μM), 0.89 mL of 0.2 mol L -1 PBS buffer solution (pH=4.0), incubate at room temperature in the dark for 15 min; then, add 40 μL of 10 mg mL to the mixed solution -1 The TMB solution was irradiated with a green LED light for 15 minutes, and the color of TMB was photocatalyzed and oxidized; the color of Hg in the water sample was obtained by comparing the color of the environmental water sample with the standard series. 2+ Concentration range, to determine the Hg in environmental water samples 2+ Whether it exceeds the national standard value.

[0020]

Embodiment 2

[0022] Add 10 μL of 10 μM DNA solution (sequence 5’-CGCATTCAGGATTCTCTACTCGTA-3’, 5’-TTCGTGTTGTGTTTCCTGTTTGCG-3’) and 1 mL of Hg 2+ Standard solution / environmental water sample, 0.89 mL with a concentration of 0.2 mol L -1 PBS buffer solution (pH=4.5), 100 μL of 4 mM ethidium bromide solution (final concentration of 50 μM), incubate at room temperature for 30 min in the dark; add 40 μL of 10 mg mL -1 The TMB solution in the water sample was irradiated with a green LED light for 30 min; the color of the Hg in the water sample was obtained by comparing the color of the environmental water sample with the standard series. 2+ Concentration range, to determine the Hg in environmental water samples 2+ Whether it exceeds the national standard value.

[0023]

[0024] Using Examples 1 and 2 to measure Hg in drinking water, tap water, irrigation water, and standard water samples (GSBZ 50016-90) 2+ The concentration is shown in Table 1, which is in good agreement with the me...

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PUM

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Abstract

The invention relates to a method for in-situ detecting Hg<2+> in a water sample through a photocatalysis visible manner. The method is characterized in that Hg<2+> is specifically combined with thymine (T) to form a -Hg<2+>-T structure, so that two T-enriched CpG-oligodeoxynucleotides are matched with each other in a staggering manner to form double strands. ethidium bromide (EB) can be embedded in the double strands, and 3,3',5,5'-tetramethyl benzidine (TMB) is oxidized under the radiation of a green LED (light emitting diode) lamp into blue. The semi-quantitative analysis of mercury ions can be realized by observing the color variation of the solution. By adopting the method, 0.05ngmL<-1> of Hg<2+> in the water sample can be detected through eyes, and the determination requirement of the Hg<2+> (1ngmL<-1>) in water samples such as drinking water, irrigation water for farmland and the like can be met. The method is good in selectivity, high in sensitivity, capable of avoiding the complicated separation and enriching process and directly applicable to the in-situ analysis of Hg<2+>.

Description

technical field [0001] The invention relates to Hg in a water sample 2+ The on-site visual detection method belongs to the field of environmental analysis and biosensing technology. Background technique [0002] Hg 2+ Is one of the highly toxic heavy metal ions, ingesting high concentrations of Hg 2+ It can cause serious damage to the brain, nervous system, endocrine system and other organs. Traditional mercury ion detection methods mainly include atomic absorption method, atomic emission method, atomic fluorescence method, inductively coupled plasma mass spectrometry and so on. However, these methods involve the use of expensive instruments, cannot analyze Hg on site 2+ . In recent years, studies have shown that Hg 2+ Can specifically covalently combine with thymine (T) in the oligonucleotide chain to form a stable T-Hg 2+ -T structure. Based on Hg 2+ Due to this characteristic, a gold nanoparticle visualization method has been developed for the determination of Hg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 张信凤刘微微许淑霞张惠
Owner CHENGDU UNIVERSITY OF TECHNOLOGY
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