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Solid state fermentation method for cordyceps sinensis

A technology of solid-state fermentation and Cordyceps sinensis, which is applied in the field of fermentation, can solve the problems of difficult removal of the medium, unpleasant smell, etc., and achieve the effect of overcoming the stirring problem of solid fermentation, low hardness and viscosity, and loose texture

Active Publication Date: 2014-07-23
四川藏宝虫草生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since commercialization requires a large amount of Cordyceps sinensis cells, the difficulty lies in the solid fermentation of Cordyceps sinensis mycelia. Traditional liquid fermentation uses silkworm chrysalis powder, yeast extract, peptone and other industrial raw materials to ferment Cordyceps sinensis cells to effectively solve the problem of production, but as Food raw materials are not the best production method as food raw materials due to their disadvantages of bad smell and residual medium that are not easy to remove

Method used

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  • Solid state fermentation method for cordyceps sinensis

Examples

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Effect test

Embodiment 1

[0017] A method for solid-state fermentation of Cordyceps sinensis, carried out according to the following steps:

[0018] (1) Preparation of solid medium: get 250g corn, 250g rice, 175g chaff, 6.75g soybean oil, 5g oyster shell, 650ml deionized water; stir evenly, and pack in 10 tissue culture bottles;

[0019] (2) Place the tissue culture bottle in a high-pressure steam cooker and sterilize at 121°C for 30 minutes;

[0020] (3) Production of strains: remove the strains on the slope, break them into pieces, make them into liquid shake flasks, culture them at 150 rpm at 18°C ​​for 5 to 10 days, and obtain liquid bacterial balls;

[0021] (4) Strain treatment and inoculation: Disperse the prepared liquid bacterial balls, inoculate them in tissue culture bottles, and cultivate them at 18°C;

[0022] (5) Mycelium growth: grow after culturing at 18°C ​​for 60 days.

Embodiment 2

[0024] A method for solid-state fermentation of Cordyceps sinensis, carried out according to the following steps:

[0025] (1) Solid medium preparation: get 100g rice, 400g red sorghum, 175g chaff, 6.75g soybean oil, 5g oyster shells, 650ml deionized water, stir evenly, and pack in 10 tissue culture bottles;

[0026] (2) Place the tissue culture bottle in a high-pressure steam cooker and sterilize at 121°C for 30 minutes;

[0027] (3) Production of strains: remove the strains on the slope, break them into pieces, make them into liquid shake flasks, culture them at 150 rpm at 18°C ​​for 5 to 10 days, and obtain liquid bacterial balls;

[0028] (4) Strain treatment and inoculation: Disperse the prepared liquid bacterial balls, inoculate them in tissue culture bottles, and cultivate them at 18°C;

[0029] (5) Mycelium growth: grow after culturing at 18°C ​​for 60 days.

Embodiment 3

[0031] A method for solid-state fermentation of Cordyceps sinensis, carried out according to the following steps:

[0032] (1) Solid medium preparation: get 150g rice (with shell), 550g red sorghum (with shell), 6.75g soybean oil, 5g oyster shell, 800ml of water, stir evenly, and pack in 10 tissue culture bottles;

[0033] (2) Place the tissue culture bottle in a high-pressure steam cooker and sterilize at 121°C for 30 minutes;

[0034] (3) Production of strains: remove the strains on the slope, break them into pieces, make them into liquid shake flasks, culture them at 150 rpm at 18°C ​​for 5 to 10 days, and obtain liquid bacterial balls;

[0035] (4) Strain treatment and inoculation: Disperse the prepared liquid bacterial balls, inoculate them in tissue culture bottles, and cultivate them at 18°C;

[0036] (5) Mycelium growth: grow after culturing at 18°C ​​for 60 days.

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Abstract

The invention discloses a solid state fermentation method for cordyceps sinensis, which belongs to the technical field of fermentation. According to the method, corn, rice, rice husk, soybean oil, oyster shell and crab grass powder are adopted as components of a culture medium, prepared seeds are subjected to solid state fermentation, the culture medium is low in hardness and viscosity, the solid fermentation stirring problem is overcome, the culture medium is floppy and breathable, the growth speed of Chinese hirsutella sinensis is effectively increased, and cordyceps sinensis mycelia produced in a fermentation mode are non-toxic, free of environment pollution, environmental friendly and low in residue, and can be directly used as food production raw materials.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for solid-state fermentation of Cordyceps sinensis. Background technique [0002] Cordyceps sinensis (Cordyceps sinensis), also known as Cordyceps sinensis, is a complex of the subunit and larvae of the fungus of the genus Cordyceps of the family Ergotaceae parasitizing on the larvae of the green bat moth. Cordyceps sinensis is distributed in the alpine meadow area of ​​3000-5000m in my country's Tibet, Qinghai, Yunnan, Sichuan and Gansu provinces, and has extremely special habitat requirements. Under natural conditions, its life history is extremely complex, and it must go through relatively independent transitions between the conidia stage and the ascospore stage. Its host bat moth takes 5 to 6 years to complete a generation, and more than 85% of the time it lives underground , with generational overlap throughout the year. Cordyceps sinensis is warm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 冯繻亿吴丽娟
Owner 四川藏宝虫草生物科技有限公司
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