Method for extracting active ingredient of folium artemisiae argyi and application of active ingredient in resistance of plant disease
A technology for active ingredients and plant diseases, applied in the field of botanical pesticides, can solve problems such as economic losses in agricultural production, short duration of effect, and loss of efficacy of copper preparations
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Embodiment 1
[0027] Embodiment 1, the extraction method of the active ingredient of Artemisia annua is carried out according to the following steps successively:
[0028] (1) Heat and reflux 20kg of dried and crushed Artemisia annua in 85% industrial ethanol for 2 times, each time for 2.5 hours. After filtering, heat and reflux the filter residue with 70% industrial ethanol for 2 hours and filter. Combined, and then concentrated under reduced pressure to remove alcohol to obtain ethanol extract.
[0029] (2) Add a small amount of distilled water to the crude ethanol extract, disperse it in an ultrasonic instrument, then extract it with petroleum ether, chloroform, ethyl acetate, and n-butanol in sequence, and concentrate under reduced pressure to remove the solvent to obtain a petroleum ether layer extract, chloroform layer extract, ethyl acetate layer extract, n-butanol layer extract and aqueous layer extract.
[0030] (3) Dissolve the above-mentioned chloroform layer extract in absolute...
Embodiment 2
[0040] Compound 8 α -Hydroxyartemisinin Anti-cucumber mosaic virus activity test (half-leaf blight method).
[0041] Anti-cucumber mosaic virus activity test test is carried out in the following steps:
[0042] (1) Virus purification
[0043] Using the Gooding method (Gooding G V jr, Hebert T T.A simple technique for purification of tobacco mosaic virus in large quantities [J]. Phytopathology, 1967, 57, 1285.), select the inoculation for more than 3 weeks, and the CMV system infects the host Nicotianatabacum. L The upper leaves of the plant were homogenized in phosphate buffer, then homogenized with 10% chloroform:n-butanol (1:1), filtered through double-layer gauze, and centrifuged at 1000g to obtain the crude extraction liquid of CMV. The whole experiment was carried out at 4°C (Junichi Fukami, Yasuhiko Uesugi [Japanese], Li Shuzheng, Wang Duku, Jiao Shumei translated. Experimental Methods of Pesticides - Fungicides [M] Beijing: Agricultural Press, 1991, 93-94 .). Measur...
Embodiment 3
[0055] Compound 8 α -Hydroxyartemisinin Anti-tobacco mosaic virus activity test (half-leaf blight method).
[0056] Anti-tobacco mosaic virus activity test test is carried out in the following steps:
[0057] (1) Virus purification:
[0058] Using the method of Gooding et al. (Gooding G. V. jr; Hebert T T., A simple technique for purification of tobacco mosaic virus in large quantities [J]. Phytopathology , 1967 , 57(11): 1285-1290.), select inoculation for more than 3 weeks, TMV system infects the upper leaves of the host Nicotiana tabacum. Treat with polyethylene glycol, centrifuge again, and suspend the precipitate with phosphate buffer to obtain the crude extraction of TMV. The whole experiment was carried out at 4°C (Junichi Fukami, Yasuhiko Uesugi [Japanese], translated by Li Shuzheng, Wang Duku, Jiao Shumei. Pesticide Experimental Methods - Fungicides [M]. Beijing: Agricultural Press , 1991 , 93-94.). Measure the absorbance value at 260nm wavelength with an ult...
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