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Bacillus coagulans strain and integrated process for producing lactic acid by using same through synchronous saccharification and fermentation of lignocellulose

A technology of bacillus coagulans and lignocellulose, which is applied in the fields of fermentation, chemical industry, and microbial-based methods, etc., can solve the problems of industrial application limitations, increase in fermentation process costs, etc., achieve strong inhibitor tolerance and avoid extensive washing Water demand, effects of avoiding energy losses

Active Publication Date: 2014-03-26
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

PCT patent PCT / US2005 / 006774 announced the use of Bacillus coagulans (Bacillus coagulans) 36D1 to ferment xylose and glucose in the detoxified bagasse dilute acid hydrolyzate, and reported the use of the strain on commercial microcrystalline cellulose SSF process, and reported the use of detoxified bagasse dilute acid hydrolyzate and microcrystalline cellulose to carry out the saccharification co-fermentation process (Simultaneous saccharification and co-fermentation, SSCF), but the detoxification process increases the cost of the fermentation process and industrialization application is limited

Method used

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  • Bacillus coagulans strain and integrated process for producing lactic acid by using same through synchronous saccharification and fermentation of lignocellulose
  • Bacillus coagulans strain and integrated process for producing lactic acid by using same through synchronous saccharification and fermentation of lignocellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Isolation and identification of Bacillus coagulans CGMCC No. 7635 and its fermentation performance

[0025] The medium used in this experiment is as follows:

[0026] MRS medium: tryptone 10g / L, beef powder 10g / L, yeast powder 5g / L, anhydrous sodium acetate 5g / L, hydrogen citrate diamine 2g / L, dipotassium hydrogen phosphate 2g / L, sulfuric acid heptahydrate Magnesium 0.2g / L, manganese sulfate monohydrate 0.05g / L. Solid medium, 1.5% agar powder is added to MRS medium, 2% glucose or xylose is added as needed; Screening medium, solid medium is added 0.015% bromocresol purple; Seed medium: peptone 10g / L, yeast Powder 5g / L, glucose 10g / L, the balance is water. Fermentation medium: tryptone 10g / L, yeast powder 5g / L, carbon source is different according to different experiments, specifically one of glucose, xylose, mannose, arabinose, galactose or cellobiose, carbon source concentration It is 20g / L, and the balance is water.

[0027] Fermentation method: inoculate the colony cultu...

Embodiment 2

[0037] Bacillus coagulans (Bacillus coagulans) CGMCC No.7635 simultaneous saccharification and co-fermentation of wheat straw integrated process for producing lactic acid

[0038] The wheat straw is washed, dried, and crushed into powder with an average size of 40 mesh. Take 100g of dry weight wheat straw powder and treat it with 2% (w / v) sulfuric acid at 121°C for 90 minutes in a fermenter, with a solid-liquid ratio of 1:10 (mass volume ratio). After pretreatment, after adjusting the pH to 6.0 with sodium hydroxide, 10g / L dry corn steep powder was added, and the prepared Bacillus coagulans CGMCC No. 7635 secondary seed solution (the preparation method is the same as in Example 1) A 5% (v / v) inoculum was inoculated into the wheat straw pretreatment liquid obtained above, and the integrated process of simultaneous saccharification and co-fermentation was started. The fermentation process is divided into phase I and phase II. The stage I culture conditions are: 300 rpm, 55°C, and...

Embodiment 3

[0040] Integrated process for simultaneous saccharification and co-fermentation of corn stover to produce lactic acid from Bacillus coagulans CGMCC No.7635

[0041] The corn stalks are washed, dried, crushed into powder with an average size of 40 meshes, and pretreated by steam explosion with ammonia. The corn stalk powder is treated by ammonia steam explosion. The steam explosion operation conditions are: pressure 1.5MPa, maintenance for 10 minutes, solid-liquid ratio 1:8 (mass volume ratio), and ammonia water consumption is 5%. After pretreatment, use sulfuric acid to adjust the pH to 7.5, then add 10g / L corn steep liquor, and prepare the Bacillus coagulans CGMCC No. 7635 secondary seeds (the preparation method is the same as in Example 1) with 5% (V / v) The amount of inoculum inoculation begins to ferment. The fermentation process is divided into phase I and phase II. The stage I culture conditions are: 300 rpm, 58°C, and the pH is temporarily not controlled. As the fermenta...

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Abstract

The invention relates to a Bacillus coagulans strain and an integrated process for producing lactic acid by using the same through synchronous saccharification and fermentation of lignocellulose, belonging to the field of biochemical engineering. According to the invention, lactic acid is produced by using the Bacillus coagulans CGMCC No.7635 strain separated from soil through bioconversion of lignocellulose. The method provided by the invention has the following advantages: the strain has a high-temperature fermentation characteristic, has high tolerance to fermentation inhibitor in lignocellulose hydrolysis liquid and can efficiently produce lactic acid from hexose, pentaglucose and cellobiose. According to the process provided by the invention, raw materials can be pretreated by using the Bacillus coagulans CGMCC No.7635 strain; lignocellulose acidolysis liquid fermentation and cellulose saccharification fermentation processes are integrated; and steps of solid-liquid separation and raw material liquid detoxification are omitted. Thus, the method can simplify the operating process, save the equipment investment, improve the fermentation efficiency and realize the high-valued application of lignocellulose, thereby having important industrial application prospects.

Description

Technical field [0001] The invention belongs to the field of biochemical industry and relates to an integrated process for producing lactic acid by synchronous saccharification and co-fermentation using lignocellulose as a raw material, so as to realize the high-value utilization of lignocellulose resources and reduce the production cost of lactic acid. Background technique [0002] Lactic acid is an important organic acid, which is widely used in food, medicine, chemical, brewing and textile fields. In recent years, polylactic acid plastic synthesized with lactic acid as a monomer is considered to be one of the best substitutes for petrochemical plastics due to its biodegradable properties. Lactic acid is mainly produced by fermentation. The microorganisms currently reported in the literature for producing lactic acid are mainly fungi Rhizopus and bacteria Lactobacillus, Streptococcus, Leuconostoc , Enterococcus (Enterococcus) and Bacillus (Bacillus). Lactobacillus bacteria ar...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/14C12P7/56C12R1/07
CPCY02P20/10
Inventor 万印华张玉明陈向荣齐本坤沈飞苏仪
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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