Application of microbial preparation for quickly decomposing cattle faeces

A microbial preparation and beef cattle technology, applied in the direction of microorganism-based methods, microorganisms, applications, etc., can solve the problems of low cellulose degradation rate, low degree of decomposition, long fermentation cycle, etc., and achieve high fiber content, low calorific value, fine texture effect

Inactive Publication Date: 2015-07-01
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a microbial preparation for rapid decomposing of beef manure and its preparation method in order to solve the problems of slow heating of manure, low cellulose degradation rate, low degree of decomposing and long fermentation period in the existing beef manure decomposing fermentation process and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0015] Specific embodiment one: the microbial preparation of beef cattle feces decomposing rapidly in the present embodiment is by the weight of each freeze-dried bacteria powder by 5-7 parts of long thorn hair shell, 4-6 parts of Trichoderma korningen, 3-5 parts of round yeast Hair shell, 3 to 5 parts of Trichoderma viride and 2 to 3 parts of Geotrichum candidum.

[0016] The 5 strains of Trichoderma longifolia, Trichoderma koningensis, Trichoderma rotundum, Trichoderma viride and Geotrichum candidum described in this embodiment were all purchased from the China General Microorganism Culture Collection and Management Center (CGMCC). The preservation number of (Chaetomium dolichotrichum) is CGMCC No: 3.3616; the preservation number of Corning Trichoderma (Trichoderma koningii) is CGMCC No: 3.4033; the preservation number of round yeast hair shell (Chaetomium torulosum) is CGMCCNo: 3.3617; viride) is CGMCC No: 3.3744; Geotrichum candidum is CGMCC No: 2.1062.

[0017] Making or...

specific Embodiment approach 2

[0018] Specific embodiment two: the microbial preparation of beef cattle feces decomposing fast in the present embodiment is by the long thorn hair shell of 6 parts, the Trichoderma korningen of 5 parts, the round yeast hair shell of 4 parts, 4 parts by each freeze-dried bacteria powder weight Made of Trichoderma viride and 2 parts of Geotrichum candidum.

specific Embodiment approach 3

[0019] Specific embodiment three: the preparation method of the rapidly decomposed microbial preparation of beef cattle manure in this embodiment is implemented according to the following steps:

[0020] 1. Sterilize the PDA medium and wort liquid medium at 121°C for 20 minutes, then cool to 30°C, and then sterilize the PDA medium, Trichoderma korningen, Trichoderma viride, and Trichoderma viride 4 strains were inoculated into 4 parts of PDA medium, and 1 strain of Geotrichum candidum was inoculated into wort liquid medium, and cultured on a shaker at 28°C for 24 hours to obtain 5 strains of mother starter;

[0021] 2. Prepare 10% skim milk emulsion, add 2% liquid glycerin, 1% xylo-oligosaccharide, and 1% glucose to the skim milk emulsion, sterilize at 121°C for 20 minutes, cool to 30°C, press 2% Inoculate 5 strains of mother starter respectively in volume ratio, culture on a shaker at 28°C for 48 hours, and obtain 5 strains of production starter;

[0022] Three, the 5 produc...

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Abstract

The invention discloses a microbial preparation of quickly decomposing cattle faeces as well as a preparation method and an application of the microbial preparation, and relates to a microbial preparation capable of decomposing cattle faeces as well as a preparation method and application of the microbial preparation. The invention aims to solve the problem that in the existing decomposing and fermenting process of cattle faeces, the manure is quick to warm, the degradative rate for cellulose is low, the decomposing degree is not high and the fermenting period is long. The microbial preparation is prepared from long thorned chaetomium, trichoderma koningii, chaetomium torulosum Bainier, Trichoderma viride and cryytococcus neoformans. The preparation method comprises the following steps: I, inoculating a strain on a culture medium; II, preparing a skimmed milk emulsion, inoculating after sterilization and producing a leavening agent on a table; III, carrying out quick freezing on the leavening agent and freezing and drying to obtain the freeze-drying culture powder; and IV, proportioning the freeze-drying culture powder according to weight part to prepare the microbial preparation after uniformly mixing. By fermenting cattle faeces with the microbial preparation, the microbial preparation is quick to warm, and can improve the degree of cellulose degradation and the degree of decomposition of organic fertilizer products and shorten the fermenting period.

Description

technical field [0001] The invention relates to a microbial preparation capable of decomposing beef cattle manure, a preparation method and application thereof. Background technique [0002] In recent years, my country's beef cattle breeding industry has developed rapidly, but a large amount of feces and sewage have been produced, which not only affects the sustainable development of related industries, but also causes serious rural environmental pollution and endangers human health. At present, domestic and foreign manure treatment methods tend to produce organic fertilizers, and the main processes include direct drying, natural composting and microbial inoculant fermentation. Cow dung contains 14.5% organic matter, nitrogen (N) 0.30-0.45%, phosphorus (P 2 o 5 )0.15-0.25%, potassium (K 2 O) 0.10-0.15%. Compared with other livestock, the organic matter and nutrient content in cow dung is the lowest among all kinds of livestock. At the same time, the texture is fine and de...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G1/00C05F17/00C12R1/645C12R1/885
CPCY02W30/43Y02W30/40
Inventor 刘春龙姜天瑞李宝山李忠秋张帆任延铭王玉珠
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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