Method for preparing undenatured natural collagen from sucking pig skin
A technology of natural collagen and skin collagen, which is applied in the field of preparation of biomedical materials, can solve the problems of lack of collagen fibers, difficulty in removing fat, and inapplicability to the preparation of undenatured natural suckling pig skin collagen, so as to ensure biological activity and low immunity original effect
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Embodiment 2
[0043] (1) Raw material pretreatment
[0044] Take 100g of fresh suckling pig skin, remove the subcutaneous fat, and treat it with isopropanol with a material-to-liquid ratio of 1:10 (w / v) and a volume concentration of 45% for 36 hours, accompanied by an intermittent power of 150W ultrasonic wave every 20 minutes and stop for 10 minutes. Repeat 8 times, change the medium 4 times in the middle, rinse with tap water, add the protease Nowolase NG solution with a material-to-liquid ratio of 1:2.5 (w / v), and a volume concentration of 1.5%, keep the temperature at 35°C, rotate for 45 minutes to remove hair, and then Chop the skin, add a solution of sodium deoxycholate with a ratio of material to liquid of 1:30 (w / v), and a concentration of 0.3M / L sodium deoxycholate, stir for 36 hours, change the solution 4 times in the middle, rinse with tap water, adjust the pH to 8.0, and measure Ash content 0.2%. Then use a material-liquid ratio of 1:10 (w / v), volume concentration of 35% aceton...
Embodiment 3
[0051] (1) Raw material pretreatment
[0052] Take 100g of fresh suckling pigskin, remove the subcutaneous fat, and treat it with AXAUT AP with a solid-liquid ratio of 1:5 (w / v) and a volume concentration of 60% for 48 hours, accompanied by intermittent power of 200W ultrasonic waves every 10 minutes and stop for 5 minutes, repeat 10 times, change the liquid 5 times in the middle, rinse with tap water, add the protease Basozyme L10 solution with a solid-liquid ratio of 1:1.5 (w / v) and a volume concentration of 3%, keep the temperature at 45°C, and rotate for 60 minutes for hair removal, then put Cut the skin into pieces, add a solution of sodium deoxycholate with a ratio of material to liquid of 1:40 (w / v) and a concentration of 0.05M / L, stir for 48 hours, change the solution 5 times in the middle, rinse with tap water, adjust the pH to 9.0, and measure the ash content Content 0.2%. Then use a material-liquid ratio of 1:6 (w / v), volume concentration of 50% acetone and AXAUT-5...
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