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Detection method for Gymnadenia conopsea medicinal material

A detection method and technology for ginseng medicinal materials, applied in the field of medicine, can solve the problems of unstable experimental results, complicated and cumbersome operations, poor representativeness, etc., and achieve the effects of simple and feasible method, high sensitivity and strong specificity.

Active Publication Date: 2014-02-12
SHANDONG JINHE DRUG RES DEV
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AI Technical Summary

Problems solved by technology

For the method of Document 1, it is difficult to obtain the standard products of the four components, and the operation is complicated and cumbersome, the experimental results are unstable and the repeatability is poor; for the method of Document 2, the method of detecting the content of only one component is difficult for complex components. The detection of Chinese herbal medicines is less representative

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  • Detection method for Gymnadenia conopsea medicinal material
  • Detection method for Gymnadenia conopsea medicinal material
  • Detection method for Gymnadenia conopsea medicinal material

Examples

Experimental program
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Effect test

experiment example 1

[0032] Experimental example 1, TLC identification method

[0033] Take ginseng control medicinal material (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd., batch number 111021) powder 0.5g, hand ginseng medicinal material sample (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd., batch number 120801) powder 0.5g, Compound Shoushen Pills (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd., batch number 20111215) powder 3g, the parallel operation is as follows: add 10ml of water-saturated n-butanol, ultrasonic treatment for 30min, filter, evaporate the filtrate to dryness, add 2ml of water to the residue and make Dissolve, pass through a D101 macroporous resin column (inner diameter 1.5cm, column height 12cm), wash with 20ml of water, discard the filtrate, elute with 25ml of 70% ethanol, collect the filtrate, evaporate to dryness, and dissolve the residue in 1ml of 70% methanol to obtain Control medicinal material solution, test solution 1, and test solution 2; draw 10...

experiment example 2

[0035] Experimental example 2, the assay method of total polysaccharide

[0036] 1. Instruments, reagents and test samples

[0037]Instruments: electronic balance: Shimadzu AUW220D; Shimadzu UV-2450 ultraviolet spectrophotometer.

[0038] Reference substance: anhydrous glucose reference substance. (National Institute of Pharmaceutical and Biological Products; batch number: 110833-200503)

[0039] Sample: hand ginseng medicinal materials (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers are: 120801, 120802, 120803.

[0040] 2. Determination of total polysaccharide content

[0041] Preparation of control solution: Take 10 mg of anhydrous glucose reference substance, put it in a 100 ml volumetric flask, add water to dissolve and dilute to the mark, shake well, and obtain.

[0042] Preparation of standard curve: Accurately measure 0ml, 0.1ml, 0.3ml, 0.5ml, 0.8ml, 1ml of the above-mentioned control solution, put them in 10ml stoppered test tubes, add water t...

experiment example 3

[0056] Experimental example 3, determination of characteristic peaks of hand ginseng and determination method of gastrodin content

[0057] 1. Instruments, reagents and test samples

[0058] Instruments: high performance liquid chromatography: Agilent 1260; electronic balance: Shimadzu AUW220D.

[0059] Reference substance: gastrodin reference substance (National Institute for the Identification of Pharmaceutical and Biological Products, batch number: 110807-200205.)

[0060] Sample: hand ginseng medicinal materials (provided by Qinghai Jinhe Tibetan Medicine Co., Ltd.), batch numbers: 120801, 120802, 120803, 1200804, 120805, 120806.

[0061] 2. Liquid chromatography conditions

[0062] Use octadecylsilane bonded silica gel as filler, detection wavelength 222nm, column temperature 40°C, theoretical plate number should not be less than 2000 based on gastrodin peak, acetonitrile as mobile phase A, add 0.1ml phosphoric acid per 100ml The 0.05mol / L potassium dihydrogen phosphat...

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Abstract

The invention relates to a detection method for a Gymnadenia conopsea medicinal material. The Gymnadenia conopsea medicinal material is detected by one or more of thin layer chromatographic identification, polysaccharide content detection, feature spectrum peak and gastrodin content detection methods; the thin layer chromatographic identification method has high specificity and high sensitivity; the internal quality of the Gymnadenia conopsea medicinal material can be further measured by performing content detection on polysaccharide and gastrodin; feature spectrum with pertinence and the detection method thereof are selected through tests, so that the Gymnadenia conopsea can be detected accurately.

Description

technical field [0001] The invention relates to a detection method of medicinal materials, in particular to a detection method of hand ginseng medicinal materials, and belongs to the technical field of medicine. Background technique [0002] Ginseng, Tibetan name "Wangla", is the tuber root of Gymnadenia conopsea (L.) R.Br., a plant of the genus Gymnadenia conopsea (L.) R.Br., which has a good effect of replenishing essence and marrow, enhancing physical fitness, promoting body fluid and quenching thirst, and calming nerves. It is commonly used in Tibetan and Mongolian medicine prescriptions for invigorating the kidney. [0003] At present, there are few researches on the detection methods of hand ginseng, and the foundation is weak. The literature "HPLC Determination of the Contents of 4 Active Components in Tibetan Medicine Ginseng Wangla" (Chinese Journal of Traditional Chinese Medicine, Issue 14, 2009, referred to as "Document 1") introduced a method for the simultaneou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N21/31G01N30/02
Inventor 魏永义任松鹏董玉波周忠山赵延霞
Owner SHANDONG JINHE DRUG RES DEV
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