Modified hyaluronic acid based macromolecule lipidosome and preparation method thereof

Abstract

The invention relates to a modified hyaluronic acid based macromolecule lipidosome and a preparation method thereof. The grain size of the macromolecule lipidosome is 80-150 nm, and the Zeta potential is between minus 36 mV and minus 40 mV. The stearic acid modified hyaluronic acid is dissolved into dichloromethane to form an oil phase; the oil phase is subjected to ultrasonic operation at the power of 100-250 w, deionized water is added, then an ultrasonic generator is used for conducting ultrasonic dispersion to form a uniform emulsion disperse system; the emulsion is subjected to rotary evaporating on a rotary evaporator at the temperature of 25-50 DEG C and at the rotating speed of 20-100 r / min, and the macromolecule lipidosome is obtained after an organic solvent is completely volatilized. The grain size of the prepared macromolecule lipidosome is less than 150 nm and is uniform, and the dispersibility is good. The macromolecule lipidosome can freely move in blood so as to reduce the cytotoxic effect caused by positive electricity. The whole preparation process is simple and convenient, the preparation period is short, the yield is high, and the preparation method is applicable to batch production.

Description

technical field [0001] The invention relates to a polymer liposome based on modified hyaluronic acid and a preparation method thereof, belonging to the technical field of medicines. Background technique [0002] Liposomes are spherical particles with a diameter of tens of nanometers to several microns, which are formed by dispersing phospholipids in water. In 1965, Bangham et al. discovered liposomes, and liposomes were first used as drug carriers in the 1970s. Its bilayer structure can effectively wrap hydrophilic, lipophilic and macromolecular drugs, and can be used as a carrier for insoluble drugs to increase its local concentration; drugs are embedded in liposomes and released slowly, and liposomes in blood circulation The drug has a longer residence time than the free drug, thus prolonging the action time of the drug and having a long-term effect; the drug will improve the stability of the encapsulated drug due to liposome encapsulation, and it can also protect and dir...

AI Technical Summary

Problems solved by technology

[0004] However, traditional liposomes are usually composed of small phospholipid molecules, which have some limitations. For example, the natural lecithin constituting the liposome membrane is easily hydrolyzed, has poor physical and chemical stability, and is difficult to store for a long time. At the same time, the encapsulated drug is easy to leak and cause burst release; In addition, traditional liposomes have disadvantages such as few functional groups, difficulty in surface modification, and single function.

The recent emergence of polymer liposomes has effectively solved some of the shortcomings of traditional liposomes, but most existing polymer liposomes often have higher cytotoxicity and poor biocompatibility due to the problems of the materials themselves.

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