Preparation method for small-particle-size magnetic agarose microspheres
A technology of agarose microspheres and small particle sizes, applied in the fields of magnetism of organic materials/organic magnetic materials, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the problem of wide particle size distribution and large particle size and other issues, to achieve the effect of wide application prospects and good application prospects
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[0027] The preparation method of the small-diameter magnetic agarose microspheres of the present invention comprises the following steps:
[0028] Step 1, the solution formed by dissolving the emulsifier in a hydrophobic organic solvent is used as the oil phase. Wherein, the volume percentage of the emulsifier in the hydrophobic organic solvent is 0.5% to 4%; the hydrophobic organic solvent includes saturated alkanes with a boiling point greater than 90°C. As known to those skilled in the art, the saturated alkanes Including but not limited to n-heptane, n-octane or n-nonane. The emulsifier includes but not limited to Span 85 and Span 80.
[0029] Step 2, agarose is added to the superparamagnetic Fe-containing 3 o 4 In the aqueous solution, the agarose is completely dissolved through vortex oscillation, ultrasonic dispersion, and microwave heating, and the resulting mixed solution is used as the water phase. The superparamagnetic Fe 3 o 4 The surface is covered with a che...
Embodiment 1
[0036] Preparation of the oil phase: Use a 500mL graduated cylinder to measure 400mL of n-octane into a 1000mL three-neck flask, and then add 12mL of Span 85. The three-necked flask was placed in a constant temperature water bath at 95°C, and stirred by electric machinery for 30 minutes.
[0037] Preparation of the aqueous phase: Weigh 4.0g of agarose powder with an analytical balance and add it to a 500mL conical flask, then add 100mL of Fe 3 o 4 / SiO 2 For an aqueous solution with a content of 10 mg / mL, vortex for 3 minutes, and then sonicate for 3 minutes, put the Erlenmeyer flask into a microwave oven and heat to dissolve the agarose.
[0038] Adjust the stirring speed in the oil phase containing n-octane to 1000rpm, slowly add the prepared aqueous phase agarose solution into the oil phase, stir for 2min, and stop stirring; quickly pour the stirred emulsion into the preheated to 100°C~ Place the beaker in a 1000mL beaker at 120°C for 8 minutes in an ultrasonic breaker, ...
Embodiment 2
[0040] Preparation of the oil phase: Use a 500mL graduated cylinder to measure 400mL of n-octane into a 1000mL three-neck flask, and then add 12mL of Span 85. The three-necked flask was placed in a constant temperature water bath at 95°C, and stirred by electric machinery for 30 minutes.
[0041] Preparation of the aqueous phase: weigh 3.0 g of agarose powder with an analytical balance and add it to a 500 mL Erlenmeyer flask, then add 100 mL of Fe 3 o 4 / SiO 2 For an aqueous solution with a content of 10 mg / mL, vortex for 3 minutes, and then sonicate for 3 minutes, put the Erlenmeyer flask into a microwave oven and heat to dissolve the agarose.
[0042] Adjust the stirring speed in the above-mentioned n-octane oil phase to 1500rpm, slowly add the prepared aqueous phase agarose solution into the oil phase, stir for 3 minutes, and stop stirring; quickly pour the stirred emulsion into the preheated to 100~120°C Put the beaker into an ultrasonic breaker for 10 minutes, and then...
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