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Preparation method for small-particle-size magnetic agarose microspheres

A technology of agarose microspheres and small particle sizes, applied in the fields of magnetism of organic materials/organic magnetic materials, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the problem of wide particle size distribution and large particle size and other issues, to achieve the effect of wide application prospects and good application prospects

Active Publication Date: 2015-07-22
BEAVERNANO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the magnetic agarose microspheres prepared by this method have better magnetic properties, the particle size is relatively large, usually greater than 20 μm, and the particle size distribution is very wide.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The preparation method of the small-diameter magnetic agarose microspheres of the present invention comprises the following steps:

[0028] Step 1, the solution formed by dissolving the emulsifier in a hydrophobic organic solvent is used as the oil phase. Wherein, the volume percentage of the emulsifier in the hydrophobic organic solvent is 0.5% to 4%; the hydrophobic organic solvent includes saturated alkanes with a boiling point greater than 90°C. As known to those skilled in the art, the saturated alkanes Including but not limited to n-heptane, n-octane or n-nonane. The emulsifier includes but not limited to Span 85 and Span 80.

[0029] Step 2, agarose is added to the superparamagnetic Fe-containing 3 o 4 In the aqueous solution, the agarose is completely dissolved through vortex oscillation, ultrasonic dispersion, and microwave heating, and the resulting mixed solution is used as the water phase. The superparamagnetic Fe 3 o 4 The surface is covered with a che...

Embodiment 1

[0036] Preparation of the oil phase: Use a 500mL graduated cylinder to measure 400mL of n-octane into a 1000mL three-neck flask, and then add 12mL of Span 85. The three-necked flask was placed in a constant temperature water bath at 95°C, and stirred by electric machinery for 30 minutes.

[0037] Preparation of the aqueous phase: Weigh 4.0g of agarose powder with an analytical balance and add it to a 500mL conical flask, then add 100mL of Fe 3 o 4 / SiO 2 For an aqueous solution with a content of 10 mg / mL, vortex for 3 minutes, and then sonicate for 3 minutes, put the Erlenmeyer flask into a microwave oven and heat to dissolve the agarose.

[0038] Adjust the stirring speed in the oil phase containing n-octane to 1000rpm, slowly add the prepared aqueous phase agarose solution into the oil phase, stir for 2min, and stop stirring; quickly pour the stirred emulsion into the preheated to 100°C~ Place the beaker in a 1000mL beaker at 120°C for 8 minutes in an ultrasonic breaker, ...

Embodiment 2

[0040] Preparation of the oil phase: Use a 500mL graduated cylinder to measure 400mL of n-octane into a 1000mL three-neck flask, and then add 12mL of Span 85. The three-necked flask was placed in a constant temperature water bath at 95°C, and stirred by electric machinery for 30 minutes.

[0041] Preparation of the aqueous phase: weigh 3.0 g of agarose powder with an analytical balance and add it to a 500 mL Erlenmeyer flask, then add 100 mL of Fe 3 o 4 / SiO 2 For an aqueous solution with a content of 10 mg / mL, vortex for 3 minutes, and then sonicate for 3 minutes, put the Erlenmeyer flask into a microwave oven and heat to dissolve the agarose.

[0042] Adjust the stirring speed in the above-mentioned n-octane oil phase to 1500rpm, slowly add the prepared aqueous phase agarose solution into the oil phase, stir for 3 minutes, and stop stirring; quickly pour the stirred emulsion into the preheated to 100~120°C Put the beaker into an ultrasonic breaker for 10 minutes, and then...

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Abstract

The invention discloses a preparation method for small-particle-size magnetic agarose microspheres. According to the preparation method, emulgator and hydrophobic organic solvent serve as an oil phase, mixed liquor of agarose and superparamagnetic Fe3O4 aqueous solutions serves as an aqueous phase, the aqueous phase is added into the oil phase under mechanical stirring to carry out pre-dispersion, then ultrasonication is carried out on pre-dispersion emulsion, and cooling, magnetic separation and purification are carried out on the crushed emulsion to obtain the small-particle-size magnetic agarose microspheres. The magnetic agarose microspheres prepared according to the preparation method have high magnetic response and superparamagnetism, and compared with commercially available products, the magnetic agarose microspheres have smaller particle sizes, larger specific surface areas, more active sites, and broad application prospects. Due to the fact that hydrophilic magnetic cores are adopted, through the modes of vortex vibration, ultrasonic dispersion, microwave heating, mechanical stirring pre-dispersion, ultrasonication carried out by a cell disruption instrument and the like, not only can the fact that the magnetic cores can be uniformly dispersed in the agarose solutions be guaranteed, but also the fact that the obtained magnetic agarose is small in particle size is guaranteed, and distribution is narrow.

Description

technical field [0001] The invention relates to a preparation method of small-diameter magnetic agarose microspheres, belonging to the field of biological materials. Background technique [0002] In recent years, magnetic polysaccharide microspheres have been widely used in the fields of biology, chemistry, pharmacy, and medicine. Their main applications include separation and purification of biomolecules such as proteins, antibodies, and enzymes, targeted drugs, immunoassays, cell separation, and chemical analysis. Agarose gel is a natural polysaccharide chromatography medium, which has many characteristics of an ideal medium, such as: high hydrophilicity, porosity, more activatable hydroxyl groups, and no non-specific adsorption with biological macromolecules. It is by far the most widely used chromatographic medium. Since agarose contains more activatable hydroxyl groups, different ligands can be inserted under certain conditions as media for affinity chromatography, hyd...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/16C08L5/12C08K9/10C08K3/22B01J20/24B01J20/28H01F1/42
Inventor 任辉秦炜谭建雄
Owner BEAVERNANO TECH
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