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Feed additive comprising lysozyme, pesticide composition and application of feed additive

The technology of a feed additive and composition is applied in the field of feed additives containing lysozyme, which can solve the problems of limited use of lysozyme, high usage of lysozyme, and impact on body recovery, so as to prevent and control animal diarrhea and stimulate the body's immune level , enhance the effect of self-resistance

Active Publication Date: 2013-12-25
ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, previous studies have limited use of lysozyme, high usage of lysozyme, high cost, and the use of antibiotics such as florbenicol has an impact on the recovery of the body
The study also found that probiotics in animals have a certain tolerance to lysozyme. At a concentration of 25mg / kg, lysozyme will not affect the growth of Lactobacillus thermophiles and Streptococcus, but if the tolerance concentration of probiotics exceeds the range, lysozyme will have a certain inhibitory effect on probiotics

Method used

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  • Feed additive comprising lysozyme, pesticide composition and application of feed additive
  • Feed additive comprising lysozyme, pesticide composition and application of feed additive
  • Feed additive comprising lysozyme, pesticide composition and application of feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment one: the antibacterial test of lysozyme

[0026] The requirement of the lysozyme used in the present application is: through the susceptibility test, there should be an obvious bacteriostatic zone at 100ppm, that is, the diameter of the bacteriostatic zone>10mm. The drug susceptibility test method is as follows: Different media can be selected for the drug susceptibility test of different bacteria, such as common nutrient agar or wheat chaff medium for the drug susceptibility test of Escherichia coli. Serum medium can be selected for Salmonella.

[0027] 1. Preparation of general LB medium

[0028] 1. Preparation of liquid culture medium

[0029] Dissolve 1g of peptone, 1g of NaCl, and 0.5g of yeast extract (products from foreign companies are recommended) in 100ml of water.

[0030] 2. Preparation of solid medium

[0031] On the basis of the liquid medium, add 1.7 g of agar or 2 g of agar powder per 100 ml.

[0032] 2. Sterilization

[0033] The medium...

Embodiment 2

[0050] Embodiment 2: piglet dysentery test

[0051] (1) Preventive effect experimental design

[0052] The 28-day-old piglets were randomly divided into 10 groups, and 5g of additives were ingested each time, 4 times a day. Respectively control group (no feed additive added); test group 1, test group 2, test group 3, test group 4, test group 5; comparison group 1, comparison group 2, comparison group 3, comparison group 4; Three repetition groups, the test period was 4 weeks. To prevent diarrhea and dysentery, piglets with the same body condition were selected for the challenge test, and the preventive effects are shown in Table 2.

[0053] Test group 1: the additive contains lysozyme, the concentration is 25ppm, and there are 3% compound oil and 2% traditional Chinese medicine composition in the additive; the compound oil is DHA and linseed oil, and the ratio of DHA to linseed oil is 1 : 10; the Chinese medicine composition is 25 parts of Atractylodes macrocephala, 14 part...

Embodiment 3

[0071] Embodiment 3: Piglet high fever test

[0072] In the early stage of high fever in pig farms, the further deterioration of high fever can be prevented by feeding the feed additives of test groups 1-4 and feed containing lysozyme. The results of disease control in the early stage of high fever are shown in Table 3.

[0073] Table 3 Treatment results in the initial stage of febrile disease

[0074]

[0075] As can be seen from the above table, feed additives with a content of lysozyme above 50ppm have achieved an ideal control effect on disease control, while it can be seen from Example 1 that the antibacterial effect of using 50ppm lysozyme alone is not obvious. It can be seen that through the synergistic effect of the compound oil and the traditional Chinese medicine composition, a lower lysozyme is used to achieve a better therapeutic effect and effectively control the initial state of high fever.

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Abstract

The invention relates to a feed additive comprising lysozyme, a pesticide composition and application of the feed additive. The lysozyme, compound grease and a traditional Chinese medicine composition are combined for use, the dosage of the lysozyme is controlled and the additive amount in the feed is regulated correspondingly; due to the synergistic effect of the lysozyme, the compound grease and the traditional Chinese medicine composition, by utilizing good antibacterial and sterilizing effects of the lysozyme and the immunoregulation, free radical regulation and assisting effect of the compound grease and the traditional Chinese medicine composition on an organism, the organism is regulated from the inside to the outside, so that the feed additive and the pesticide composition can resist inflammation and infection effectively and improve autoimmunity and resistance to diseases, also can kill strains having tolerance to antibiotic, guarantees the health of intestinal tracts of animals, prevents and cures animal diarrhea, and improves the production performance of animals. The feed additive and the pesticide composition are good in curative effect, high in safety and low in cost, have no drug resistance, no drug residue and no toxic and side effects, and are environment-friendly active substances.

Description

technical field [0001] The invention relates to a livestock and poultry feed additive and a pharmaceutical composition, in particular to a feed additive containing lysozyme and its application. Background technique [0002] Piglet diarrhea is a typical multifactorial disease in pig production. According to statistics, the incidence rate of piglet diarrhea is 35%, and its indirect loss is manifested in cured piglet growth retardation and low feed conversion rate, which seriously threatens the pig industry. development of. Since May 2006, pig hyperthermia has broken out successively throughout the country, causing a large number of pigs to become ill and die, resulting in significant economic losses. Up to now, high fever is still prevalent in pig herds in 22 provinces of the country, endangering the healthy growth of pigs and threatening the development of pig industry. The adverse consequences of the use of antibiotics in farming have been fully recognized by everyone. Ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/14A23K1/165A61K38/47A61P1/12A61P31/04A61P29/00A61P37/04
Inventor 潘宏涛
Owner ZHEJIANG AEGIS BIOLOGICAL SCI & TECH CO LTD
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