Bacterium for degrading herbicide chlorimuron-ethyl and application of bacterium
A technology for chlorflusulfuron and a bacterial agent, which is applied in the field of bacteria for degrading the herbicide chlorflusulfuron, can solve the problems of aging of branches and stem bases, loss of agricultural production, delayed maturity and the like
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Embodiment 1
[0038] Example 1. Isolation and Identification of Enterobacter sp. LMHL1716CGMCC No.7772
[0039] 1. Isolation of chlorimuron-methyl-degrading bacteria LMHL1716
[0040] Add 10 g of soil samples (collected from farmland polluted by the herbicide chlorimuron-methyl in Shaanxi Province, China) to 100 mL of enrichment culture solution containing 20 mg / L of chlorimuron-methyl, and culture for 7 days at 28°C and 180 r / min with shaking to enrich chlorine rimsulfuron-methyl-degrading bacteria. After the first round of enrichment, 10 mL of the enrichment solution was transferred to 100 mL of the enrichment medium containing 40 mg / L of chlorimuron-methyl, and the culture was continued for 7 days for the second round of enrichment. Such continuous enrichment and culture for 5 times, the concentrations of chlorimuron-methyl were 20, 40, 60, 80 and 100 mg / L in turn.
[0041] The screening of degrading bacteria adopts the plate transparent circle method. Add chlorimuron-methyl to inorga...
Embodiment 2
[0063] Example 2, Quantitative Determination of Enterobacter sp. LMHL1716CGMCC No.7772 Degradation Chlorsulfuron-methyl Ability
[0064] 1. Preparation of standard curve for the determination of chlorimuron-methyl
[0065] Use methanol to prepare chlorimuron-methyl standard substance (purchased from Fluka Company) into a series of standard solutions with concentrations of 10, 20, 40, 60, 80, and 100 mg / L, and use high performance liquid chromatography (HPLC) to determine different concentrations of chlorimuron-methyl The peak area of the standard was repeated three times. With the concentration of chlorimuron-methyl as the abscissa and the peak area as the ordinate, draw the chlorimuron-methyl standard curve.
[0066] The detection conditions are as follows:
[0067] Detection system: Agilent1100Series. Chromatographic column: C18Diamosil TM Reversed-phase column, 250mm×4.6mm, particle size 5μm. Chromatographic conditions: mobile phase: acetonitrile: methanol: water: gl...
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