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Collagen-based dura and preparation method thereof

A dura mater and collagen technology, applied in the field of collagen-based dura mater and its preparation, can solve the problems of difficult practical application requirements of dura mater substitutes, loss of collagen biological activity, unfriendly production process, etc., to achieve easy formation of large-scale industries Chain, sufficient source, low price effect

Active Publication Date: 2013-08-28
BEIJING HOTWIRE MEDICAL TECH DEV CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] To sum up, the dura mater substitutes prepared with collagen as the main material still have deficiencies in the preparation process or product performance, while the production process is unfriendly when electrospinning technology is used, and most of the biological activity of collagen The defects of loss and insufficient mechanical properties, while the pure acellular matrix material has the risk of leakage due to the pore structure running through it
It can be seen that it is difficult for a single-material dura mater substitute to meet this seemingly contradictory practical application requirement at the same time, and it seems inevitable to take the road of composite materials

Method used

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  • Collagen-based dura and preparation method thereof
  • Collagen-based dura and preparation method thereof
  • Collagen-based dura and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of collagen homogeneous electrospinning solution: firstly, NaH2PO4 with a mass fraction of 0.1%wt was dissolved in 1-allyl-3-methylimidazolium benzoate ([BMIM][Ac] ), to obtain the ionic liquid composite solvent system, gradually dissolve bovine hide type I collagen in the ionic liquid composite solvent system at 4°C, initially add 1% of the mass percentage of the ionic liquid composite solvent system, and then add bovine hide Type I collagen, until the mass percentage of cowhide type I collagen in the ionic liquid composite solvent system is 5%, ultrasonic defoaming at 25°C until completely free of bubbles;

[0045] (2) Preparation of conductive decellularized porcine dermis substrate: split fresh ecological pigskin into thin slices with a thickness of 0.5-1.0 mm, and ultrasonically treat them with peracetic acid with a mass concentration of 3.0% for 1 h to inactivate the virus. Immediately afterwards, 50,000 units of trypsin with an activity mass conce...

Embodiment 2

[0051] (1) Preparation of collagen homogeneous electrospinning solution: first, dissolve NaHSO4 with a mass fraction of 0.5%wt in 1-ethyl-3-methylimidazolium glycinate ([EMIM][Gly]) at room temperature 25°C to obtain Ionic liquid composite solvent system, gradually dissolve pigskin type I collagen in the ionic liquid composite solvent system at 10°C, initially add 3% of the mass percentage of the ionic liquid composite solvent system, and then add pigskin I in turn after it is fully dissolved Collagen type I, until the mass percentage content of pigskin type I collagen in the ionic liquid composite solvent system is 10%, ultrasonic defoaming at 25°C until completely free of bubbles;

[0052] (2) Preparation of conductive decellularized porcine dermis substrate: the decellularized porcine dermis used was obtained in Example 1, and the composition was 0.9%wt sodium chloride (NaCl), 0.01Mol / L phosphate buffer solution ( PBS), the volume ratio NaCl: PBS is 5:5 electrolyte buffer...

Embodiment 3

[0058] (1) Preparation of collagen homogeneous electrospinning solution: firstly, NaHCO3 with a mass fraction of 1%wt was dissolved in 1-allyl-3-methylimidazole benzoate ([Amim][Ba] ), to obtain the ionic liquid composite solvent system, gradually dissolve bovine Achilles tendon type I collagen in the ionic liquid composite solvent system at 20°C, initially add 5% of the mass percentage of the ionic liquid composite solvent system, and then sequentially Add bovine Achilles tendon type I collagen until the mass percentage of bovine Achilles tendon type I collagen in the ionic liquid composite solvent system is 15%, and stand at 25°C for defoaming until completely free of bubbles;

[0059] (2) Preparation of conductive decellularized porcine dermis substrate: the decellularized porcine dermis used was obtained from Example 1, and the composition was 0.9%wt sodium chloride (NaCl), 0.01Mol / L phosphate buffer solution ( PBS), the volume ratio of NaCl: PBS is 9:1 electrolyte buffer ...

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Abstract

The invention provides a collagen-based dura on the basis of a process-friendly type ion liquid on-site spinning technology, and a preparation method thereof. The preparation method comprises the steps of: dissolving I type collagen by a novel process-friendly solvent system-ion liquid and sodium salt composite system, fully soaking by taking decellularized pig dermis as a substrate through an electrolyte solution, carrying out electrostatic spinning by taking air as a medium, and regulating the pore structure of collagen fibers by adjusting the electrospinning conditions; removing residual ion liquid with a proper eluent, and carrying out freeze-drying, sterilization and packaging to obtain the collagen-based dura. The collagen-based dura has two or more layers of structures: the aperture of collagen fibers at one side close to brain tissue is controlled to be 3mu m and below, and the aperture of collagen fibers at one side far away from brain tissue is controlled to be 20mu m and above. Compared with the traditional solvent spinning, the preparation method is process-friendly, the toxic and side effects are reduced, and the triple-helical structure of the collagen can be furthest retained.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a collagen-based dura mater and a preparation method thereof. Background technique [0002] The dura mater is composed of dense connective tissue and attached to the inner surface of the skull. It is a double-layer fibrous membrane. Its main function is to protect the brain and participate in the formation of fluid-filled cavities around the brain. It is essential for maintaining the normal physiological functions of the brain. important. Dural defect repair is relatively common in neurosurgery. Factors such as open craniocerebral injury, inflammation, brain swelling, brain tumors invading the meninges, and excessive electrocautery may cause the dura mater to fail to close and form a defect. The defect of the dura mater can lead to complications such as cerebrospinal fluid leakage, intracranial infection, delayed healing of the incision, and seizures. Therefore, ...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/24D04H13/00D01D5/00
Inventor 胡杨但卫华但年华富勇马文杰
Owner BEIJING HOTWIRE MEDICAL TECH DEV CO LTD
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