A kind of urease nanoreactor and preparation method thereof

Abstract

The invention discloses a urease nano-reactor which comprises the effective components of ultraviolet light initiated azide cross-linked carboxymethyl chitosan derivatives and urease. The preparation method of the urease nano-reactor comprises the following steps of: adding urease into a carboxymethyl chitosan solution; according to the mole ratio of the structural unit of the carboxymethyl chitosan to 4-azide benzaldehyde being1:(0.01-10), adding an ethanol solution of 4-azide benzaldehyde into the aqueous solution containing enzyme carboxymethyl chitosan, shielding from the light and stirring at normal temperature so as to obtain a photo-crosslinking carboxymethyl chitosan amphiphilic polymer, and when being made into hollow nano-capsules in a self-assembling mode, in situ packaging urease; and finally carrying out ultraviolet light initiated azide cross-linking on the nano-capsule solution so as to obtain the enzyme-wrapped biologic nano condensed capsule, namely the urease nano-reactor. The preparation method of the urease nano-reactor is simple and convenient; the urease nano-reactor has good stability and semipermeability; due to the characteristics similar to a cell membrane, the urease nano-reactor shows strong attractive potential in the application fields such as bionic nano devices.

Description

technical field [0001] The invention relates to the field of bionic materials, in particular to a urease nanoreactor and a preparation method thereof. Background technique [0002] Enzyme nanoreactor is a kind of nano reaction system with bionic cell structure and enzyme metabolism function. It encapsulates enzymes in a nanoscale polymer with a specific structure. The polymer has a hollow capsule structure, and its outer shell is similar to a cell membrane, which allows some small molecules (such as enzyme substrates) to permeate freely, but can Avoiding the leakage of packaged macromolecules (such as enzymes and genes) can also prevent the infiltration of active macromolecules (such as enzymes) in the environment. It can not only be used for the replacement therapy of a certain enzyme deficiency in the patient's body, but also be used for the catalytic metabolism of the poison in the body, thereby prolonging the survival period of the patient. These properties make enzyme...

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Problems solved by technology

[0008] (1) Most of the constituent materials of the enzyme nanoreactor prepared by the above method have no biodegradability and poor biocompatibility, which is not conducive to the metabolism of the enzyme nanoreactor in the body, which has greatly restricted the application of the enzyme nanoreactor. limit

[0009] (2) The preparation process of the enzyme nanoreactor prepared by the above method is quite complicated (free radical polymerization reaction, homogeneous polymerization reaction), and the process requires the use of organic solvents and harsh reaction conditions, which is not conducive to the maintenance of enzyme activity

[0011] (4) The enzyme nanoreactor prepared by the above method is not stable, and it is easy to disintegrate prematurely in the body, resulting in enzyme leakage

Due to the limitations of the stability and scale of active macromolecular enzymes, it is difficult to carry active enzymes in nanoreactors.

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