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Method for extracting pleurotus eryngii polysaccharide from pleurotus eryngii leftover by using subcritical water

A technology for the leftovers of Pleurotus eryngii and the polysaccharide of Pleurotus eryngii is applied in the field of extracting the polysaccharide of Pleurotus eryngii and using subcritical water to extract the polysaccharide of Pleurotus eryngii. The complex components of the polysaccharide extract can achieve the effects of good permeability and solubility, low production cost, and less loss of active ingredients and active substances.

Inactive Publication Date: 2013-06-05
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic hydrolysis extraction method has the advantages of high polysaccharide extraction rate, mild reaction conditions, and little damage to the polysaccharide structure. However, due to the high price of enzymes and relatively high production costs, it is not suitable for large-scale industrial production.
In addition, ultrasonic extraction and microwave extraction can increase the extraction rate of polysaccharides and shorten the extraction time, but sometimes the components of the polysaccharide extract are more complex, which brings certain difficulties to the subsequent separation and purification process
In addition, the Savag method is mostly used to remove protein from edible fungus polysaccharides. This method not only uses toxic organic solvents such as chloroform and n-butanol, but also has inconvenient recovery and high cost, and cannot be adapted to industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Grind the dried Pleurotus eryngii leftovers into 40 mesh particles, take 100g and put it into a subcritical water extraction kettle, replace the oxygen in the extraction kettle with nitrogen, and input 1000mL of deoxidized deionized water at 180°C into the extraction kettle through a pressure pump. The pressure of the extraction kettle is 5MPa, heat preservation and extraction for 20min, after filtration, cooling, and centrifugation, the subcritical water extract is ultrafiltered with a ceramic membrane with a molecular weight cut-off of 100kD, and the operating pressure is 0.5MPa. The membrane is subjected to ultrafiltration, the operating pressure is 0.1MPa, and 50ml of the intercepted concentrated solution is obtained, which is put on the pretreated D301 anion exchange resin column, and 300ml of deionized water is added for elution, and the polysaccharide eluate is collected, concentrated under reduced pressure and dried in vacuo , to obtain 6.8g Pleurotus eryngii pol...

Embodiment 3

[0032] Grind the dried Pleurotus eryngii leftovers into 60-mesh particles, take 100g and put it into a subcritical water extraction kettle, replace the oxygen in the extraction kettle with nitrogen, and input 1000ml of deoxidized deionized water at 160°C into the extraction kettle through a pressure pump. The pressure of the extraction kettle is 4MPa, after heat preservation and extraction for 30min, after filtration, cooling, and centrifugation, the subcritical water extract is ultrafiltered with a hollow fiber membrane with a molecular weight cut-off of 300kD, and the operating pressure is 0.1MPa. The fiber membrane is subjected to ultrafiltration, the operating pressure is 0.1MPa, and 50ml of the intercepted concentrated solution is obtained, which is put on a pretreated 201×7 anion exchange resin column, and 200ml of deionized water is added for eluting, and the polysaccharide eluate is collected, concentrated under reduced pressure and After vacuum drying, 6.6g Pleurotus e...

Embodiment 4

[0034] Select fresh and clean Pleurotus eryngii leftovers, cut into thin slices of about 2mm, take 1000g and put it into a subcritical water extraction kettle, replace the oxygen in the extraction kettle with nitrogen, and input 5000ml of 140°C deoxidized deionized water into extraction through a pressure pump In the kettle, the pressure of the extraction kettle is 3MPa. After heat preservation and extraction for 30min, after filtration, cooling and centrifugation, the subcritical water extract is ultrafiltered with a ceramic membrane with a molecular weight cut-off of 300kD, and the operating pressure is 0.4MPa. The hollow fiber membrane is subjected to ultrafiltration, the operating pressure is 0.1MPa, and 50ml of intercepted concentrated solution is obtained, and the pretreated D318 anion exchange resin column is added, and 400ml of deionized water is added for elution, and the polysaccharide eluate is collected, concentrated under reduced pressure and decompressed. After fr...

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Abstract

The invention provides a method for extracting pleurotus eryngii polysaccharide from pleurotus eryngii leftovers by using subcritical water. The method comprises the following steps of: feeding the pleurotus eryngii leftovers into an extraction kettle; feeding deoxidized deionized water according to a material-liquid mass volume ratio of 1:(5-30); controlling the extraction pressure to be 3-10MPa, the temperature to be 100-250 DEG C and the extraction time to be 10-60min; filtering, cooling and centrifuging; carrying out ultrafiltration by using an ultrafiltration film of which the molecular weight cut-off is 100-300kD; cutting off the filtrate by using an ultrafiltration film of which the molecular weight cut-off is 3-10kD; feeding the crude polysaccharide cut-off liquid into an anion exchange resin column; and eluting by using deionized water; and concentrating under reduced pressure and drying the collected polysaccharide eluate so as to obtain a pleurotus eryngii polysaccharide product. According to the method, a subcritical water extraction technique is combined with the separation means of ultrafiltration edulcoration and resin absorption decoloration, no organic solvents are used in the whole process, the extraction process is green and environment-friendly, and the pleurotus eryngii polysaccharide is high in purity and is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for extracting pleurotus eryngii polysaccharides from leftovers of pleurotus eryngii, in particular to a method for extracting pleurotus eryngii polysaccharides by using subcritical water, and belongs to the technical field of intensive processing of agricultural and sideline products. Background technique [0002] Pleurotus eryngii ( Pleurotus eryngii ), also known as celery, has a snow-white fruiting body and a crisp and tender texture. It has the reputation of "Oyster Mushroom King", "Scallop Mushroom", and "Delicious Boletus on the Grassland". Modern pharmacological studies have shown that the polysaccharides contained in Pleurotus eryngii have the effects of anti-oxidation, lowering blood fat, inhibiting tumors, anti-virus, anti-allergic, anti-aging, lowering cholesterol and enhancing the body's immunity. Both fruit body and mycelium of Pleurotus eryngii can be used as raw materials for polysaccharide extraction....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 郑光耀周鹭红何玲黄永恒薄采颖
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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