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Method for cultivating antrodia camphorate sporocarp basswood in the second stage

A cultivation method, the technology of Antrodia camphorata, applied in the field of fermentation engineering, can solve the problems of unrevealed basswood utilization rate, insufficient vitality, low competitiveness, etc., achieve industrialized cultivation methods, increase speed and efficiency, and solve osmotic pressure problem effect

Inactive Publication Date: 2013-05-22
SHENZHEN RENTAI BIOLOGICAL TECH
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the slow growth characteristics of Antrodia camphorata, many basswood cultivations have low competitiveness after inoculation, difficulty in survival, long cultivation period (generally about 2 years), low conversion rate (generally around 10-20%), and low yield And other issues
Although Antrodia camphorata is a brown rot fungus, its brown rot ability is not strong. Antrodia camphorata only rots the surface of the camphor tree, and will not rot it to death
Because the ability of Antrodia camphorata to transform wood is very low, generally only 10%-20%. Since 90% of the main components of camphor wood are lignin, cellulose and hemicellulose, Antrodia camphorata generally grows on decayed camphor trees. In fact, it is because the ability of Antrodia camphorata to decompose lignin and cellulose is relatively poor. Wood decay is because the main components of wood, lignin, cellulose and hemicellulose are decomposed to produce small molecules such as xylose, glucose, and pentose Antrodia camphorata fungus growth, and Antrodia camphorata fungus itself is not strong enough to decompose wood because the activity of lignin decomposing enzymes, cellulose, and hemicellulolytic enzymes secreted by Antrodia camphorata fungus itself is not strong enough. Generally, white rot fungi The conversion rate of linden wood is above 95%. White rot fungi such as shiitake mushrooms, fungus, and ganoderma can decompose and transform 98% of basswood, while the conversion rate of basswood of Antrodia camphorata is only 10%-20%, and the main components of wood are 90%. It is lignin, cellulose, and hemicellulose. Experiments have proved that Antrodia camphorata has a relatively strong ability to decompose cellulose and hemicellulose, but the ability to decompose lignin is very poor. Because Antrodia camphorata cannot quickly decompose lignin in wood for nutrition, Therefore, the growth is slow, because Antrodia camphorata cannot obtain the necessary nutrients for growth from other sources, and can only rely on a small amount of polysaccharides, starch and other nutrients in the camphor tree itself to maintain, and at the same time rely on itself to slowly decompose lignin, cellulose and hemicellulose, etc. To obtain the necessary nutrients for growth, so the yield of Antrodia camphorata is not high, and the growth is slow
[0005] Chinese patent ZL201010518942.0 discloses a method for cultivating Antrodia camphorata with micro-ventilated basswood. This patent uses liquid fermentation and multiplication to obtain liquid strains inoculated on the surface of camphor wood through the treatment of camphor wood. And by controlling the cultivation temperature, humidity, light and other conditions for cultivation, adopting a micro-ventilation method, it not only ensures the air permeability but also effectively prevents the contamination of bacteria, and cultivates the fruiting bodies of Antrodia camphorata; but this patent does not disclose how to improve the utilization rate of basswood Methods

Method used

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Embodiment 1

[0020] Embodiment 1 A kind of two-stage culture method of Antrodia camphorata fruiting body basswood

[0021] This embodiment adopts the strain of Antrodia camphorata: ATCC200183.

[0022] Material: camphor tree basswood, high temperature PE bag.

[0023] 1. Cultivation of Antrodia camphorata liquid strains

[0024] Slant medium: 20g malt extract, 20g glucose, 1g peptone, 0.5g magnesium sulfate, 20g agar powder, constant volume 1L, pH5.0, then add agar to prepare slant medium.

[0025] Liquid seed medium: 20g of malt extract, 20g of glucose, 1g of peptone, 0.5g of magnesium sulfate, constant volume 1L, pH 5.0.

[0026] For the cultivation of Antrodia camphorata liquid strains, the activated Antrodia camphorata slant strains were cut into 5mm-sized mycelium pieces and inoculated in the liquid seed medium, filled with 100ml culture medium in a 250ml triangular bottle, and cultivated at 120r / min, 25°C for 7 Today, make liquid bacteria for use.

[0027] 2. Make the center of ...

Embodiment 2

[0032] Embodiment 2 A kind of two-stage culture method of Antrodia camphorata fruiting body basswood

[0033] 1. Cultivation of Antrodia camphorata liquid strains

[0034] Incline medium: 20g of malt extract, 20g of glucose, 1g of peptone, 0.5g of magnesium sulfate, 20g of agar powder, constant volume 1L, pH5.0.

[0035] Liquid seed culture medium: malt extract 20g, glucose 20g, peptone 1g, magnesium sulfate 0.5g, constant volume 1L, pH 5.0.

[0036] Solid camphor powder medium: malt extract 20g, glucose 20g, peptone 1g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 1g, constant volume 1L, and then mixed with camphor wood powder evenly to make a water content of 40%-90% Aqueous solid medium.

[0037] Antrodia camphorata strain: CCRC36716.

[0038] Material: camphor basswood, high temperature PE bag.

[0039] For the cultivation of Antrodia camphorata solid strains, the activated Antrodia camphorata slant strains were cut into 5mm-sized mycelium pieces and inoculat...

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Abstract

The invention relates to the field of fungus cultivation, and provides a method for cultivating antrodia camphorate sporocarp basswood in the second stage. The method includes: artificially assisting basswoods to decay; cultivating dominant bacteria of antrodia camphorate; transplanting the antrodia camphorate to the artificially assisted decayed basswoods after the antrodia camphorate grows into a dominant bacterial community, and obtaining the cultivating antrodia camphorate sporocarp. By the aid of the method for cultivating antrodia camphorate sporocarp basswood in the second stage, the problems about transformation rate and nutrition supplement of the antrodia camphorate to the basswoods are effectively solved, so that yield rate of antrodia camphorate cultivation is effectively increased by 4-8 times, cultivation time is shortened by 5-8 months, and industrialized cultivation mode of antrodia camphorate sporocarp basswood is effectively realized.

Description

technical field [0001] The invention relates to the field of fermentation engineering, in particular to a two-stage cultivation method of antrodia camphorata fruiting body basswood. Background technique [0002] Antrodia camphorata belongs to Basidiomycetes, Polyporacea, Polyporaceae, and Leptinus genus. It is a rare medicinal fungus. Antrodia camphorata contains a variety of physiologically active components, such as polysaccharides, triterpenoids, superoxide dismutation Enzyme (SOD) adenosine, protein (including immune protein), multivitamins, trace elements, nucleic acid, lectin, amino acid, cholesterol, blood pressure stabilizing substances, etc. Its physiologically active functions include anti-tumor, enhancing immunity, anti-virus, anti-allergic, anti-hypertensive, inhibiting platelet aggregation, lowering blood pressure, lowering cholesterol, anti-bacterial, and protecting the liver. [0003] Antrodia camphorata is a species unique to Taiwan. It originally grew on th...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 赵宗杰谢海涛杨如钢
Owner SHENZHEN RENTAI BIOLOGICAL TECH
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