Method for cultivating antrodia camphorate sporocarp basswood in the second stage
A cultivation method, the technology of Antrodia camphorata, applied in the field of fermentation engineering, can solve the problems of unrevealed basswood utilization rate, insufficient vitality, low competitiveness, etc., achieve industrialized cultivation methods, increase speed and efficiency, and solve osmotic pressure problem effect
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Embodiment 1
[0020] Embodiment 1 A kind of two-stage culture method of Antrodia camphorata fruiting body basswood
[0021] This embodiment adopts the strain of Antrodia camphorata: ATCC200183.
[0022] Material: camphor tree basswood, high temperature PE bag.
[0023] 1. Cultivation of Antrodia camphorata liquid strains
[0024] Slant medium: 20g malt extract, 20g glucose, 1g peptone, 0.5g magnesium sulfate, 20g agar powder, constant volume 1L, pH5.0, then add agar to prepare slant medium.
[0025] Liquid seed medium: 20g of malt extract, 20g of glucose, 1g of peptone, 0.5g of magnesium sulfate, constant volume 1L, pH 5.0.
[0026] For the cultivation of Antrodia camphorata liquid strains, the activated Antrodia camphorata slant strains were cut into 5mm-sized mycelium pieces and inoculated in the liquid seed medium, filled with 100ml culture medium in a 250ml triangular bottle, and cultivated at 120r / min, 25°C for 7 Today, make liquid bacteria for use.
[0027] 2. Make the center of ...
Embodiment 2
[0032] Embodiment 2 A kind of two-stage culture method of Antrodia camphorata fruiting body basswood
[0033] 1. Cultivation of Antrodia camphorata liquid strains
[0034] Incline medium: 20g of malt extract, 20g of glucose, 1g of peptone, 0.5g of magnesium sulfate, 20g of agar powder, constant volume 1L, pH5.0.
[0035] Liquid seed culture medium: malt extract 20g, glucose 20g, peptone 1g, magnesium sulfate 0.5g, constant volume 1L, pH 5.0.
[0036] Solid camphor powder medium: malt extract 20g, glucose 20g, peptone 1g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 1g, constant volume 1L, and then mixed with camphor wood powder evenly to make a water content of 40%-90% Aqueous solid medium.
[0037] Antrodia camphorata strain: CCRC36716.
[0038] Material: camphor basswood, high temperature PE bag.
[0039] For the cultivation of Antrodia camphorata solid strains, the activated Antrodia camphorata slant strains were cut into 5mm-sized mycelium pieces and inoculat...
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