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Artificial skin graft for genipin immobilized activin B and preparation method thereof

A technology of artificial skin and graft, applied in the field of artificial skin graft and preparation, can solve the problems of affecting wound care effect, lack of durability, reduced water retention capacity, etc., and achieve prevention of pathogenic microorganism invasion, good biocompatibility, and convenience. Effects of storage and transportation

Active Publication Date: 2013-05-08
广州优特佳生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In response to this situation, many new dressings have appeared in recent years. The widely acclaimed one is the polymer hydrogel skin wound repair dressing, which has good air permeability and water permeability, can resist the invasion of bacteria, and can adapt to the shape of the wound. However, the disadvantages of molding are that the colloid has poor strength and lacks durability.
However, the synthetic dressing is thermally reversible, and it is easy to deform when the temperature is high, making it difficult to transport and store, and the freezing and thawing process will easily cause the water in the hydrogel dressing to overflow, resulting in a greatly reduced water retention capacity and absorption of wound exudate. The ability of the skin is also reduced, which affects its wound care effect, and it is not easy to fit with the skin
Hydrogel dressings synthesized by radiation method, due to the low mechanical strength of the hydrogel prepared by radiation method, especially when a single raw material is used for irradiation, the resulting gel is relatively brittle

Method used

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  • Artificial skin graft for genipin immobilized activin B and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A preparation method for an artificial skin graft of genipin-fixed activin B, comprising the following steps:

[0023] 1) Raw material purification:

[0024] Weigh 5g gelatin and dissolve it in 100mL distilled water, heat to 50°C and stir to dissolve;

[0025] Weigh 10g carboxymethyl chitosan and dissolve in 100mL distilled water, stir to dissolve at room temperature;

[0026] The dissolved gelatin and carboxymethyl chitosan solutions were centrifuged at a high speed of 15,000 rpm for 5 minutes, separated to obtain supernatants, and freeze-dried for 24 hours to obtain purified gelatin and carboxymethyl chitosan respectively;

[0027] 2) Preparation of artificial skin grafts with genipin-immobilized Activin B growth factor

[0028] Weigh 2.25g of purified gelatin and 0.25g of carboxymethyl chitosan respectively, sterilize them by ultraviolet light for 30min, then add them into sterilized distilled water, heat to 50°C, stir to dissolve and cool to room temperature, then...

Embodiment 2

[0032] A preparation method for an artificial skin graft of genipin-fixed activin B, comprising the following steps:

[0033] 1) Purify gelatin and carboxymethyl chitosan according to the method in Example 1;

[0034] 2) Preparation of artificial skin grafts:

[0035] Weigh 4.5g of purified gelatin and 0.5g of carboxymethyl chitosan respectively, sterilize by ultraviolet light for 30min, then add them into sterilized distilled water, heat to 50°C, stir to dissolve and cool to room temperature, and set Make up to 40mL to get gelatin, carboxymethyl chitosan mixture;

[0036] Weigh 25 mg of genipin and dissolve it in 10 mL of sterilized distilled water, filter and sterilize to obtain an aqueous solution of genipin;

[0037] Pour the genipin aqueous solution into the mixture of gelatin and carboxymethyl chitosan, stir well at room temperature, add 50 μL of 10 μg / mL activin B solution, stir well, pour it into a mold, and place it at 4°C to form a gel , to obtain genipin-immobili...

Embodiment 3

[0039] A preparation method for an artificial skin graft of genipin-fixed activin B, comprising the following steps:

[0040] 1) Purify gelatin and carboxymethyl chitosan according to the method in Example 1;

[0041] 2) Preparation of artificial skin grafts:

[0042] Weigh 9g of purified gelatin and 1g of carboxymethyl chitosan respectively, sterilize them by ultraviolet light for 30min, then add them into sterilized distilled water, heat to 50°C and stir to dissolve, then cool to room temperature, and make up to volume with sterilized distilled water to 40mL to obtain gelatin, carboxymethyl chitosan mixture;

[0043] Weigh 25 mg of genipin and dissolve it in 10 mL of sterilized distilled water, filter and sterilize to obtain an aqueous solution of genipin;

[0044] Pour the genipin aqueous solution into the mixture of gelatin and carboxymethyl chitosan, stir well at room temperature, add 50 μL of 10 μg / mL activin B solution, stir well, pour it into a mold, and place it at ...

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Abstract

The invention discloses an artificial skin graft for genipin immobilized activin B and a preparation method thereof. The artificial skin graft comprises hydrogel prepared from a skin graft stent material, and activin B which is distributed in the hydrogel and is cross-linked through genipin. The preparation method comprises the following steps of: sequentially adding an aqueous solution of genipin and the activin B into an aqueous solution of the skin graft stent material, uniformly mixing, forming, processing, gelatinizing and obtaining the product. The product is non-toxic, does not have pyrogenicity and has a high biocompatibility; pathogenic microorganism intrusion can be prevented, and infection incidence is reduced; quick healing of epithelial tissues can be promoted; and moreover, the exudation of wound surface fluid is reduced, a locally wet microenvironment is kept, the conventional disinfection and sterilization can be performed, and the artificial skin graft is convenient to storage and transport and is applied to burn, trauma and other skin injury repair.

Description

technical field [0001] The invention relates to an artificial skin graft and a preparation method thereof, in particular to an artificial skin graft in which genipin fixes activin B growth factor and a preparation method thereof. Background technique [0002] With the continuous progress of society and the rapid development of medicine, many diseases have been effectively controlled, but skin wounds have continued unabated. At present, millions of people across the country are injured by it. Therefore, skin trauma is a disease that cannot be ignored, such as burns or trauma, which will damage the epidermis and dermis of the skin, and even cause full-thickness skin damage. When the treatment cannot heal In the case of severe wounds, it is often necessary to permanently seal the wound by autologous skin grafting, but for large burn wounds or patients with weak wound regeneration ability, the available skin is limited; for open infected wounds, use thin The success rate of spl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/22A61L27/20A61L27/60
Inventor 张琳张璐陈世萱
Owner 广州优特佳生物科技发展有限公司
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