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Method for detecting antioxidants in vegetable oil or animal fat

A technology for antioxidant substances, animal and vegetable oils and fats, applied in the field of high performance liquid chromatography, can solve the problems of not being used to detect antioxidant substances, poor separation effect, etc., and achieve simple operation, time saving, high sensitivity and wide sources. Effect

Inactive Publication Date: 2013-04-24
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, all of the above methods use reversed-phase HPLC columns and polar eluents such as water, methanol, and acetonitrile, which are not effective in separating weakly polar materials such as animal and vegetable oils, so they have not been used to detect antioxidants in animal and vegetable oils. substance

Method used

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  • Method for detecting antioxidants in vegetable oil or animal fat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Detection of antioxidant substances in corn germ oil

[0024] 1 device

[0025] device attached figure 1 connect. The HPLC system is HP1050. Including HPLC high pressure pump, autosampler, DAD detector, equipped with AlltimaHPLC silica gel column (3μm, 150×4.6mm i.d.). Data processing with HP ChemStation. A UV-visible absorption detector (Applied Biosystems, model 785A, Forster City, USA) was connected to a recorder (Kipp & Zonen BD40; Delft, Netherlands) to record the change in absorbance of the free radical solution. Determination of galvinoxyl · The detection wavelength is 425nm. A buffer (superloop. GE Healthcare Bio-Sciences AB, Uppsala, Sweden, 150 ml) was connected to an HPLC pump (Gynkotek, 300) to deliver the free radical solution to the reaction circle through the "tee". The material of the reaction ring is PEEK, the model is 10.0m×0.25mm i.d.

[0026] 2 Sample and solvent treatment

[0027] Dissolve 1.000 g of corn germ oil in 100 mL of n-...

Embodiment 2

[0036] Example 2: Detection of antioxidant substances in olive oil

[0037] 1 device: the same as embodiment one.

[0038] 2 Sample and solvent treatment: Dissolve 1.0 g of olive oil in 100 mL of n-hexane to prepare a 10 mg / mL solution. All samples and solvents required for HPLC analysis were filtered through a 0.45 μm filter membrane.

[0039] 3 Preparation of free radical solution: same as Example 1.

[0040] 4HPLC analysis method

[0041] Eluent A: n-hexane; Eluent B: tert-butyl methyl ether, flow rate 0.8mL / min. The sample concentration is 10 mg / mL, and the injection volume is 20 μL. The detection wavelength is 292nm. The gradient elution program is 0-15min, B: 8%-12%; 15-20min, B: 12%-20%; 20-30min, B: 20%-50%; 30-32min, B: 50%- 8%; 32-45min, B: 0%.

[0042] 4 Online system parameter setting

[0043] HPLC flow rate 0.8mL / min, detection wavelength 292nm. The free radical detection wavelength is 425nm. The free radical concentration is 0.002mmol / L, the free radica...

Embodiment 3

[0046] Example 3: Detection of Antioxidant Substances in Butter

[0047] 1. Device: Same as in Example 1, but the reaction circle is changed to 30.0m in length.

[0048] 2 Sample and solvent treatment: Take 1.0 g of butter and dissolve it in 100 mL of n-hexane to prepare a 10 mg / mL solution. All samples and solvents required for HPLC analysis were filtered through a 0.45 μm filter membrane.

[0049] 3 Preparation of free radical solution: same as Example 1.

[0050] 4HPLC analysis method

[0051] Eluent A: n-hexane; Eluent B: tert-butyl methyl ether. Flow rate 1.2 mL / min. The sample concentration is 10 mg / mL, and the injection volume is 20 μL. The detection wavelength is 280nm. The gradient elution program is 0-15min, B: 0%-5%; 15-20min, B: 5%-10%; 20-30min, B: 10%-25%; 30-32min, B: 25%- 0%; 32-45 min, B: 0%.

[0052] 4 Online system parameter setting

[0053] HPLC flow rate 1.2mL / min, detection wavelength 280nm. The free radical detection wavelength is 425nm. The f...

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Abstract

The invention relates to a device for detecting antioxidants in vegetable oil or animal fat and an operating method thereof, and belongs to the technical field of high performance liquid chromatography (HPLC). The method for detecting antioxidants in the vegetable oil or animal fat can quickly detect the objects with antioxidant activity in the oil and fat. The device for detecting antioxidants in vegetable oil or animal fat is composed of a high performance liquid chromatograph (HPLC includes a high-pressure pump, a silicone liquid chromatographic column, a visible ultraviolet detector, a sampler, a signal recorder), a high-pressure pump, a visible detector, a signal recorder, a T-joint for the HPLC, a buffer, and a reaction cycle, and utilizes a chemical radical Galvinoxyl to detect antioxidant in mixture. HPLC mobile phase utilizes n-hexane and tert-butyl methyl ether, and the radical solvent is n-hexane. Components in the oil and the fat are reacted with the radical after being separated by the high performance liquid chromatography (HPLC) one by one, wherein components with antioxidant activity enable the radical to fade and to be the negative peak. Chromatograms being compared with those reacted with the radical, the number of the antioxidants in the oil and fat, retention time and the antioxidant activity of the components can be acquired. The method for detecting the antioxidants in the vegetable oil or animal fat has the advantages of being quick and easy, and high in sensitivity.

Description

1. Technical field: [0001] The invention belongs to the technical field of high performance liquid chromatography analysis, and more specifically relates to a method for online detection of antioxidant substances in animal and vegetable oils by using high performance liquid chromatography and a free radical detection system. 2. Background technology: [0002] High-performance liquid chromatography (HPLC) is an instrument and method for efficiently separating and analyzing compounds in a mixture. Equipped with different detectors, it can detect compounds of different properties in the mixture. Commonly used detectors are: ultraviolet absorption detector (UVD), diode array detector (DAD), fluorescence detector (FLD), refractive index detector (RID), evaporative light scattering detector (ELSD), mass spectrometer ( MSD). These detectors detect compounds based on certain chemical and physical properties of the compounds, which are not closely related to the activity of the comp...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 张强苏印泉朱铭强张京芳郑冀鲁
Owner NORTHWEST A & F UNIV
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