Method for preparing antrodia camphorata ferment
A technology of Antrodia camphorata enzyme and Antrodia camphorata, which is applied in the field of preparation of Antrodia camphorata enzyme, can solve the problems of changing the flavor of the enzyme, taking a long time, and not obvious efficacy characteristics, etc., and achieve the effect of less bacterial pollution and health protection
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Embodiment 1
[0024] Embodiment 1 A kind of preparation method of Antrodia camphorata enzyme
[0025] 1. Seed Culture of Antrodia Antrodia
[0026] The formula of Antrodia camphorata seed medium is: 2%-5% glucose, 1%-4% malt extract, 0.1%-0.2% yeast extract, 0.01%-0.1% peptone, PH=4.0-7.0;
[0027] Preferably: 3.5% glucose, 3% malt extract, 0.15% yeast extract, 0.05% peptone, PH=4.0-7.0
[0028] The culture medium was sterilized by damp heat at 121°C for 20 minutes, cooled to normal temperature and inoculated with 1-2 rings of Antrodia camphorata slant seeds, placed on a shaker at 22-28°C, with a rotation speed of 90-200rpm / min, and cultivated for 4-8 days to obtain shaker flask seeds .
[0029] 2. Seed Culture of Bacillus subtilis
[0030] The formula of Bacillus subtilis seed culture medium is: 1%-5% glucose, 1%-0.2% yeast extract, 0.01%-0.1% peptone, 3% soybean hydrolyzate, PH=4.0-7.0;
[0031] Preferably: 3% glucose, 0.5% yeast extract, 0.05% peptone, 3% soybean hydrolyzate, PH=6.0 ...
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