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Method for culturing bacterial cellulose through pulsation

A technology of bacterial cellulose and pulsating culture, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of short preparation period, low preparation cost and good mechanical strength

Inactive Publication Date: 2013-03-20
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method still belongs to the category of static training in essence.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1) Preparation of fermentation medium;

[0062] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;

[0063] The pH of the fermentation broth is 4.0;

[0064] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0065] 2) Bacteria expansion;

[0066] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 pieces / ml.

[0067] 3) Static cultivation;

[0068] The expanded bacterial solution was transferred to a culture container filled with fermentation culture solution and a hollow oxygen-permeable mold inside, placed in a constant temperature incubator...

Embodiment 2

[0081] 1) Preparation of fermentation medium;

[0082] Components of the fermentation medium, in mass percent, in wt%: 5 glucose, fructose, sucrose or mannitol, 0.5 peptone, 0.5 yeast extract, 0.1 citric acid, 0.2 disodium hydrogen phosphate, 0.1 potassium dihydrogen phosphate, and amount of water;

[0083] The pH of the fermentation broth is 6.0;

[0084] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0085] 2) Bacteria expansion;

[0086] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 7 pieces / ml.

[0087] 3) Static cultivation;

[0088] Transfer the expanded bacterial solution to a culture container filled with fermentation medium and a hollow oxygen-permeable mold inside, place it in a constant temperature incubator, and culture it at 32...

Embodiment 3

[0101] 1) Preparation of fermentation medium;

[0102] Components of the fermentation medium, in mass percent, in wt%: glucose, fructose, sucrose or mannitol 2, peptone 0.05, yeast extract 0.05, citric acid 0.01, disodium hydrogen phosphate 0.02, potassium dihydrogen phosphate 0.01, and amount of water;

[0103] The pH of the fermentation broth is 4.0;

[0104] The above components are mixed, sterilized by high pressure steam, irradiated with ultraviolet light and cooled to room temperature, and passed through pure oxygen to obtain the fermentation culture solution;

[0105] 2) Bacteria expansion;

[0106] The fermentation culture liquid is inoculated and expanded; the degree of expansion: the number of strain cells is 2×10 5 pieces / ml.

[0107] 3) Static cultivation;

[0108] The expanded bacterial solution was transferred to a culture container filled with fermentation culture solution and a hollow oxygen-permeable mold inside, placed in a constant temperature incubator...

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Abstract

The invention relates to a method for culturing bacterial cellulose through pulsation. According to the method, a hollow oxygen permeating die is placed into a culture container during culturing, the hollow oxygen permeating die is internally filled by air, and the pressure of the air in the hollow oxygen permeating die is periodically changed, so that the hollow oxygen permeating die can be alternatively switched to contract / release in a radial direction. The bacterial cellulose material is prepared in a 'quasi state' way under the condition that the bacterial cellulose is free from layering and is partially adhered. The inner cellulose micelles are relatively obviously orientated in the radial direction and are combined through hydrogen bonds in the molecules and the hydrogen bonds between the molecules; and the material is high in mechanical strength in the radial direction. By adopting the method, the density of the inner structure of the cellulose can be controlled, and a bacterial cellulose tubular material with a dense and smooth inner chamber can be manufactured, wherein the bacterial cellulose tubular material is regular in radial direction and is provided with a differentiation structure. The method is simple in formation process, short in preparation circle of support materials, green and environment-friendly in preparation process, simple, convenient and quick in preparation, and low in preparing cost.

Description

technical field [0001] The invention relates to a method for pulse culturing bacterial cellulose. Background technique [0002] Bacterial cellulose refers to the general term for cellulose synthesized by certain microorganisms in the genus Acetobacter, Agrobacterium, Rhizobium and Sarcina under different conditions. Acetobacter xylinum in the genus Acetobacter is widely used as a template microorganism for basic and applied research on bacterial cellulose because of its highest cellulose production efficiency. [0003] The preparation methods of bacterial cellulose are mainly static culture and dynamic culture. Due to the oxygen-dependent survival properties of the Gram-negative primary cell body, bacterial cellulose is formed at the junction of the sugar source and the aerobic region. The bacterial cellulose material obtained by static culture can form regular geometric shapes such as film and tube according to the different shapes of culture containers and application fi...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/01C12R1/02C12R1/38C12R1/41
Inventor 杨敬轩李喆郑羿王华平陈仕艳王利群
Owner DONGHUA UNIV
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