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Method for preparing immobilized enzyme on surface of polymer base material

A technology for immobilizing enzymes on the surface of substrates, applied in the directions of immobilization on/in organic carriers, textiles and papermaking, fiber processing, etc. Enzyme activity, improved mechanical strength, mild reaction conditions

Active Publication Date: 2013-02-13
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, related patents (Yang Wantai et al., CN101307122A) and papers (Journal of Polymer Science: Part A: Polymer Chemistry, 47, 6852, 2009) did not involve visible light-induced cross-linking polymerization to prepare gel / substrate composite membranes for enzymes fixed content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The LDPE membrane was extracted with acetone (extraction and washing solution) for 72 hours, and dried at room temperature. Cover the BOPP film above the LDPE film, inject 50 μL of isopropylthioxanthone in acetone solution (concentration: 0.5M) between the double-layer polymers, and then use two quartz plates to compact the double-layer polymers. Propylthioxanthone acetone solution is evenly distributed between the double-layer polymers to form a sandwich structure, and moved into the high-pressure mercury lamp irradiation device (the light intensity at the wavelength of 254nm is 9000μW / cm 2 ) at room temperature for 180 seconds. The LDPE film that has been introduced with dormant groups that can continue to initiate free radical polymerization was extracted with acetone for 1 hour to remove isopropylthioxanthone adsorbed on the surface, and dried at room temperature. Polyethylene glycol diacrylate (molecular weight 575) and glucose enzyme are jointly dissolved in a ph...

Embodiment 2

[0024] The LDPE membrane was extracted with acetone (extraction and washing solution) for 72 hours, and dried at room temperature. Cover the BOPP film above the LDPE film, inject 20 μL of isopropylthioxanthone in acetone solution (concentration: 0.3M) between the double-layer polymers, and then use two quartz plates to compact the double-layer polymers. The acetone solution of propylthioxanthone is evenly distributed between the double-layer polymers to form a sandwich structure, and moved into the medium-pressure mercury lamp irradiation device (the light intensity at the wavelength of 254nm is 5000μW / cm 2 ) at room temperature for 150 seconds. The LDPE film that has been introduced with dormant groups that can continue to initiate free radical polymerization was extracted with acetone for 1 hour to remove isopropylthioxanthone adsorbed on the surface, and dried at room temperature. Dissolve polyethylene glycol diacrylate (molecular weight 575), acrylamide and glucose enzyme...

Embodiment 3

[0027] The LDPE membrane was extracted with acetone (extraction and washing solution) for 72 hours, and dried at room temperature. Cover the BOPP film above the LDPE film, inject 30 μL of isopropylthioxanthone in acetone solution (concentration: 0.5M) between the double-layer polymers, and then use two quartz plates to compact the double-layer polymers. The acetone solution of propylthioxanthone is evenly distributed between the double-layer polymers to form a sandwich structure, and moved into the medium-pressure mercury lamp irradiation device (the light intensity at the wavelength of 254nm is 5000μW / cm 2 ) at room temperature for 200 seconds. The LDPE film that has been introduced with dormant groups that can continue to initiate free radical polymerization was extracted with acetone for 1 hour to remove isopropylthioxanthone adsorbed on the surface, and dried at room temperature. Dissolve polyethylene glycol diacrylate (molecular weight 575), hydroxyethyl methacrylate and...

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Abstract

The invention relates to a method for preparing immobilized enzyme on a surface of a polymer base material and belongs to the technical field of preparation of immobilized enzyme. The method includes the following steps: step 1, photoinitiator is absorbed on the surface of a polymer or a photoinduction dormancy group is covalent linked to the surface of the polymer; step 2, under irradiation of visible light or ultraviolet, the photoinitiator or the dormancy group generates free radicals, a monomer solution containing free enzyme is initiated to conduct controllable cross-linked polymerization reaction, and finally enzyme is immobilized on a surface cross-linked network. Enzyme immobilization is conducted under the condition of visible light and at low temperature or room temperature, and reaction conditions are mild and favorable for remaining of enzyme activity. A gel / base material composite structure improves the mechanical strength of the cross-linked network and overcomes the shortcomings that a gel network is easy to be damaged. Enzyme stability is improved, and recycling of enzyme is achieved. The method is applicable to single-enzyme immobilization and immobilization of complex enzyme.

Description

technical field [0001] The invention belongs to the technical field of preparation of immobilized enzymes, in particular to a method for immobilizing enzymes on the surface of polymer substrates triggered by visible light. Background technique [0002] Chemical reactions using enzymes as catalysts have the advantages of high catalytic efficiency, high substrate selectivity, and mild reaction conditions, and are one of the important technical means to realize green chemistry and sustainable development. However, free enzymes also have the disadvantage of variability. They tend to lose their catalytic activity under high temperature, strong acid, strong alkali, ultraviolet light and other environments, and are not easy to separate after the reaction, which not only pollutes the product, but also increases the cost because it cannot be reused. conducive to its practical application. To solve this problem, free enzymes can be immobilized. The immobilization of the enzyme makes...

Claims

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Application Information

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IPC IPC(8): C12N11/08C08J7/18C08L23/06C08L23/12C08L67/02C08L77/00C08L27/06C08L33/12C08L35/00C08L69/00C08L77/02C08L1/02C08L1/08C08L61/06C08L63/00D06M14/22D06M14/28D06M14/30D06M14/32D06M14/34
Inventor 杨万泰赵长稳马育红
Owner BEIJING UNIV OF CHEM TECH
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