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Method for degumming silybum marianum seed oil

A technology of milk thistle seed oil and degumming method, which is applied in the field of degumming of milk thistle seed oil, can solve the problems of high unsaturated fatty acid content, difficulty in oil refining, difficult to grasp the stability of refining process, etc., and achieves low phospholipid content. , High degumming efficiency, easy to control effect

Inactive Publication Date: 2013-02-13
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

It is worth noting that milk thistle hair oil is acidic, and the measured pH is generally around 5-6. It has a high content of unsaturated fatty acids, is easy to be oxidized, and is easy to combine with other macromolecules (proteins and carbohydrates) to form lipids. Compounds such as polysaccharides and lipoproteins make oil refining difficult, and the stability of the refining process is difficult to grasp

Method used

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  • Method for degumming silybum marianum seed oil
  • Method for degumming silybum marianum seed oil
  • Method for degumming silybum marianum seed oil

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Optimum test of process data for enzymatic degumming of milk thistle seed oil.

[0022] Experimental plan: through the single factor experiment of the amount of enzyme added and the reaction time of the enzyme, the optimal reaction conditions were explored.

[0023] Materials and methods 1.1 Raw materials and reagents Crude milk thistle oil was pressed, with an initial phospholipid content of 273 mg / kg; Lecitase® Ultra: A1-position phospholipase, produced by microbial fermentation, Novozyme; other reagents: analytically pure. 1.2 Main instruments Electric constant temperature water bath, JB90-D powerful electric mixer, 752 spectrophotometer, etc. 1.3 Analysis of phosphorus content Take an appropriate amount of milk thistle seed oil to analyze the phosphorus content in the oil, referring to the molybdenum blue colorimetric method in GBT 5537-2008. 1.4 Enzymatic degumming step Use a 100mL round bottom flask to accurately weigh 50g of milk thistle crude oil, heat ...

Embodiment 2

[0031] Accurately weigh 50g of milk thistle crude oil in a 100mL round bottom flask, heat the crude oil in a water bath to 70-80°C, add 45% (w / v) citric acid solution at the same temperature according to the dosage ratio of 0.12mL / 100g crude oil , 500r / min mechanical stirring for 20-30min. Then cool to 50-55°C, add 4% (w / v) NaOH solution to adjust the pH to 5-5.3. Then add phospholipase according to the amount of 60mg / kg crude oil, keep it in a water bath for 4h under the condition of mechanical stirring at 500r / min, then let it stand for 3-8h, centrifuge at 10000r / min for 10min, remove the phospholipid, and obtain the degummed milk thistle Seed oil was analyzed for phospholipid content, and the measured phospholipid content was 131.53 mg / kg.

Embodiment 3

[0033]Accurately weigh 50g of milk thistle crude oil in a 100mL round bottom flask, heat the crude oil in a water bath to 70-80°C, add 45% (w / v) citric acid solution at the same temperature according to the dosage ratio of 0.12mL / 100g crude oil , 500r / min mechanical stirring for 20-30min. Then cool to 50-55°C, add 4% (w / v) NaOH solution to adjust the pH to 5-5.3. Then add phospholipase according to the amount of 60mg / kg crude oil, keep it in a water bath for 8h under the condition of mechanical stirring at 500r / min, then let it stand for 3-8h, centrifuge at 10000r / min for 10min, remove the phospholipid, and obtain the degummed milk thistle Seed oil was analyzed for phospholipid content, and the measured phospholipid content was 51.16 mg / kg.

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Abstract

The invention discloses a method for degumming silybum marianum seed oil, relates to the technical field of refining of grease, and in particular relates to a method for degumming silybum marianum seed oil to remove nonhydratable phospholipid. The method specifically comprises acid reaction, alkali neutralization, enzyme reaction, separation and drying, wherein the acid used in the acid reaction is citric acid; and the enzyme used in the enzyme reaction is phospholipase. The method comprises the following steps: heating crude oil to 70 to 80 DEG C; adding 45 percent (w / v) citric acid solution; preserving heat for 20 to 30 minutes under the mechanical stirring condition; cooling the crude oil to 50 DEG C; adding 4 percent (w / v) NaOH solution; adjusting the pH value to 5 to 5.3; adding the phospholipase; stirring under the heat-preserving condition; and filtering or centrifugally separating. By the enzyme method degumming method, the phospholipid content of the silybum marianum seed oil is obviously reduced, the degumming efficiency is high and the advantages are obvious. The method is simple in process, mild in reaction condition, low in energy consumption and easy to control.

Description

technical field [0001] The invention relates to the technical field of oil refining, in particular to an effective degumming method for milk thistle seed oil. Background technique [0002] Milk thistle seed oil is a fatty oil obtained from the ripe fruit of the Compositae herb Silybum marianum (L.) Gaertn, accounting for about 30% of the total weight of the dry fruit, rich in linoleic acid, flax A variety of polyunsaturated fatty acids such as acid and other components such as protein, amino acid, fat, vitamins and trace elements have high nutritional value. Milk thistle seed oil is also edible abroad, called "kangaroo oil". In China, animal toxicity tests, nutrition tests and consumption have been carried out by Guangzhou Fourth Pharmaceutical Factory, Wuhan Drug Control Institute, Xi'an Botanical Garden and other units, and it has been proved that it is non-toxic and harmless, and its nutritional value is equal to that of soybean oil. Literature reports have proved that...

Claims

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Application Information

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IPC IPC(8): C11B3/04C11B3/00C11B3/06
Inventor 梅琳王亮李倩倩徐希明余江南
Owner JIANGSU UNIV
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