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Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof

A technology for anti-aging and extract, applied in the field of Gorgon Fructus extract and its preparation, can solve the problems of unstable strong oxidants and the like, and achieve the effects of less impurities, increased enzyme activity and good anti-oxidation

Inactive Publication Date: 2012-11-28
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitric oxide synthase (NOS) can catalyze the generation of NO in the body. NO is not only a neurotransmitter, but also an extremely unstable and strong oxidant. However, excessive NO deposition in the brain will cause cytotoxic reactions, which may be related to overexpression of inducible NOS

Method used

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  • Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof
  • Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof
  • Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A Gorgon fruit extract with anti-oxidation and anti-aging effects, which is prepared by the following method:

[0032] (1) Take the Gorgon fruit medicinal material, cleanly select, remove impurities, put it in a multi-functional extraction tank, add 8 times the amount of ethanol with a concentration of 80%, reflux extraction 3 times, each extraction is 8 hours, combine the extracts, filter, and obtain the filtrate , concentrated under reduced pressure, and evaporated to dryness to obtain the ethanol extract of Gorgon fruit, which was set aside.

[0033] (2) Take the ethanol extract of Gorgon fruit prepared in the above step (1), add water to suspend it, extract 6 times with the same volume of ethyl acetate to obtain the ethyl acetate extract, concentrate under reduced pressure, evaporate to dryness, and obtain Gorgon fruit Ethyl acetate extract, standby;

[0034] (3) D101 type macroporous adsorption resin on the ethyl acetate extract prepared in step (2), firstly elute...

Embodiment 2

[0036] A Gorgon fruit extract with anti-oxidation and anti-aging effects, which is prepared by the following method:

[0037](1) Take the Gorgon fruit medicinal material, cleanly select, remove impurities, put it in a multi-functional extraction tank, add 6 times the amount of ethanol with a concentration of 80%, reflux extraction 3 times, each extraction is 8 hours, combine the extracts, filter, and obtain the filtrate , concentrated under reduced pressure, and evaporated to dryness to obtain the ethanol extract of Gorgon fruit, which was set aside.

[0038] (2) Take the ethanol extract of Gorgon fruit prepared in the above step (1), add water to suspend it, extract 6 times with n-butanol of the same volume to obtain the n-butanol extract, concentrate under reduced pressure, evaporate to dryness, and obtain Gorgon fruit Butanol extract, spare;

[0039] (3) Get the D101 type macroporous adsorption resin on the n-butanol extract prepared in step (2), and first use water and a ...

Embodiment 3

[0040] Embodiment 3 anti-oxidation, anti-aging experiment

[0041] 1. Experimental materials

[0042] 1.1 Test drugs

[0043] Gorgon fruit was purchased from Beijing Zhongge Luyuan Technology Co., Ltd., batch number: 110901; the extract prepared in Example 1 and Example 2 of the present invention.

[0044] D-galactose, Shanghai Baoman Biological Co., Ltd., batch number: 110523.

[0045] GSH-Px, NOS, AChE, Coomassie Brilliant Blue protein assay kit, Nanjing Jiancheng Bioengineering Institute, batch number: 20120416.

[0046] 1.2 Test animals

[0047] Clean grade KM mice were provided by the Experimental Animal Center of Jiangsu University, animal certificate number: SCXK (Su) 2009-0002:.

[0048] 1.3 Experimental Instruments

[0049] Mouse open field device, Shanghai Ruanlong Technology Development Co., Ltd.

[0050] SLY-WMS Morris water maze, Beijing Shuolinyuan Technology Co., Ltd.

[0051] FSH-2A adjustable high-speed electric homogenizer, Jintan Jinnan Instrument Fac...

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Abstract

The invention discloses a gordon euryale seed extract with antioxidation and anti-ageing effects as well as a preparation method and application thereof. Through system optimization of an extraction solvent, an extracting method, an extraction method and a macroporous resin separation method, an optimal preparation process is obtained to prepare the gordon euryale seed extract. An experimental result shows that the gordon euryale seed extract provided by the invention is capable of obviously improving the motion capability and the exploratory behavior of a subacute ageing mouse in an open field, the academic record and memory ability of the mouse in water maze tests and the spleen and kidney indexes of the mouse, increasing the activity and protein contents of GSH-Px (Glutathione Peroxidase) and NOS (Nitric Oxide Synthase) in brain tissues, reducing the activity of AChE (acetylcholinesterase) in the brain tissues, improving the pathological change of the brain tissues, and having excellent antioxidation and anti-ageing effects.

Description

technical field [0001] The invention relates to an extraction method of a traditional Chinese medicine, in particular to a Gorgon fruit extract with anti-oxidation and anti-aging effects, a preparation method and application thereof. Background technique [0002] Gorgon fruit was first recorded in "Shen Nong's Materia Medica", and it was listed as top grade. The effect of spleen to relieve diarrhea, dispel dampness and stop leukorrhea, mainly treat nocturnal emission, slippery essence, enuresis, frequent urination, chronic diarrhea due to spleen deficiency, white turbidity, and leukorrhea. It is known as "ginseng in water" and "longan in water". It is a traditional Chinese medicinal material and Precious natural supplements. [0003] my country is one of the countries with the fastest aging rate in the world and one of the countries with the largest aging population. The national 1% population sample at the end of 2005 showed that the population over 65 years old accounted...

Claims

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Application Information

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IPC IPC(8): A61K36/62A61P39/06A61P9/10A61K31/352A61K131/00
Inventor 吴启南周婧乐巍段金廒沈蓓
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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