Monoclonal antibody for detecting aminoglycoside antibiotics and kit thereof

Abstract

The invention provides a monoclonal antibody for detecting aminoglycoside antibiotics and a kit thereof. A hybridoma cell strain Pami-SP20 with the preservation number being CCTCCC201135 is acquired; andaccording to an aminoglycoside antibiotics residue quick-detection method established by a monoclonal antibody excreted by the strain, the monoclonal antibody excreted by the monoclonal hybridoma cell strain can be directly applied in aminoglycoside antibiotics remained ELISA (Enzyme-linked Immuno Sorbent Assay) quick-detection reagent preparation and detection of an acquired antibody remained by the aminoglycoside antibiotics through a double-antibody sandwich ELISA method. The aminoglycoside antibiotics detection kit comprises a detection plate wrapped by a streptomycin-BSA (Bovine Serum Albumin) antigen, the monoclonal antibody marked with HRP (Horse Radish Peroxidase), and ELISA reaction liquid. The monoclonal antibody excreted by the monoclonal hybridoma cell strainhas the advantages of strong specificity and high affinity, and provides a new manner for quick detection of aminoglycoside antibiotics remaining.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for preparing a hybridoma cell line of a general monoclonal antibody against aminoglycoside antibiotics, and obtaining a detection kit for its residue. Background technique [0002] Aminoglycoside antibiotics are composed of two or three amino sugar molecules and one amino cyclic alcohol connected by ether bonds, mainly including streptomycin, gentamicin, neomycin, tobramycin, kanamycin It is a broad-spectrum antibiotic with strong bactericidal effect on Mycobacterium tuberculosis, Leptospira, Actinomyces and various Gram-negative bacilli, and is used in animal husbandry production It is also often added to the feed to promote growth and development. At the same time, aminoglycoside antibiotics are prone to cross-resistance. For example, Escherichia coli, which was originally sensitive to streptomycin at a concentration of 1ug / ml, can tolerate a concentratio...

AI Technical Summary

Problems solved by technology

These antibiotics have serious side effects on the human brain, nerves, hearing and kidneys.

For example, from June 1, 2002, Germany raised the streptomycin residue limit standard from the original 0.2mg / kg to 0.02mg / kg, so that all EU countries will control according to this standard, while my country According to the testing situation of honey, most of them are higher than this standard. As a result, our honey cannot be exported to EU countries or can only be sold at a reduced price, causing serious losses to our honey exporters and producers.

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