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Compound adjuvant, vaccine containing the same, and its preparation method

An adjuvant and compound technology, applied in the field of vaccine adjuvant, can solve the problems of unsatisfactory stability of W/O/W formulation vaccine, occurrence of side reactions, high price, etc., and solve the side reactions and toxic side reactions of vaccines. Reduced, good immunogenicity effect

Inactive Publication Date: 2012-07-25
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that the stability of the prepared W / O / W dosage form vaccine is not satisfactory, and the price is particularly expensive
Invention patent "A self-emulsifying vaccine adjuvant and its preparation method" (ZL 200510037639.8) discloses a series of W / O / W vaccine adjuvant preparation methods and vaccine preparation methods, but (1) side effects in field trials (2) The stability is less than 12 months, unable to meet the actual needs of production; (3) Its commercial use has not been seen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Preparation of Adjuvant

[0025] Recipe 1

[0026] White oil 80g,

[0027] Palm oil 10 g,

[0028] Polyoxyethylene oleate 5 g,

[0029] Polyoxyethylene alkyl ether 2 g,

[0030] Mannitol Oleate 3 g.

[0031] Recipe 2:

[0032] White oil 79 g,

[0033] palm oil 8 g,

[0034] Polyoxyethylene oleate 7 g,

[0035] Polyoxyethylene alkyl ether 4 g,

[0036] Mannitol Oleate 2 g.

[0037] Recipe 3:

[0038] White oil 77g,

[0039] palm oil 5 g,

[0040] Polyoxyethylene oleate 8 g,

[0041] Polyoxyethylene alkyl ether 9g,

[0042] Mannitol Oleate 1 g.

[0043] Formulas 1, 2, and 3 were used to prepare compound adjuvants in the following way: weigh white oil, palm oil, polyoxyethylene oleic acid ether ester, polyoxyethylene alkyl ether, and mannitol oleic acid ester according to the formula amount, and then Mix well, and after sterilizing at 115°C for 20 minutes, compound adjuvant 1, compound adjuvant 2 and compound adjuvant 3 were respectively obtained ...

Embodiment 2

[0044] Example 2 Application of porcine foot-and-mouth disease vaccine

[0045] (1) Preparation of compound adjuvant

[0046] Compound adjuvant 1, compound adjuvant 2 and compound adjuvant 3 were prepared according to the method in Example 1.

[0047] (2) Preparation of porcine foot-and-mouth disease virus solution

[0048] In accordance with the "Compilation of Quality Standards for Veterinary Biological Quality" (2006-2008), the inactivated porcine foot-and-mouth disease antigen solution should be prepared in accordance with the relevant requirements. 7.0 LD 50 .

[0049] (3) Preparation of porcine foot-and-mouth disease vaccine

[0050] Under aseptic operation, heat the compound adjuvant prepared in (1) above to 30°C, take 15g and put it into a 50ml beaker; ℃; then mix the two, add a magnetic rotor and place it on a magnetic stirrer (with heating function) to stir and emulsify at the speed of 2000 rpm, keep the temperature at 30°C, stir for 30 minutes, emulsify into...

Embodiment 3

[0056] Example 3 Application of porcine circovirus vaccine

[0057] (1) Preparation of compound white oil adjuvant

[0058] According to the method of Example 1, compound adjuvants 1, 2, and 3 were prepared respectively.

[0059] (2) Preparation of porcine circovirus solution

[0060] In accordance with the "Compilation of Quality Standards for Veterinary Biological Quality" (2006-2008), the inactivated porcine circovirus liquid should be prepared according to relevant requirements, and the virus content per ml of virus liquid before inactivation should be ≥10 5.5 TCID 50 .

[0061] (3) Preparation of porcine circovirus vaccine

[0062] Under aseptic operation, heat the compound adjuvant prepared in (1) above to 30°C, take 30g and put it into a 100ml glass bottle; preheat 30g of porcine foot-and-mouth disease inactivated virus solution prepared in (2) to 20°C; then mix the two, use a rod-shaped homogenizer, 12000 rpm, and emulsify for 10 minutes to form a homogeneous syst...

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PUM

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Abstract

The invention relates a compound adjuvant, which comprises mixed oil (white oil and palm oil) and mixed surfactant (polyoxyethylene oleate, polyoxyethylene alkyl ether and mannitol oleate), specifically (by weight percentage) white oil 70-90, palm oil 1-15, polyoxyethylene oleate 1-10, polyoxyethylene alkyl ether 1-10, and mannitol oleate 1-10. The compound adjuvant is mixed with antigen aqueous solution, and emulsified under stirring to obtain W / O / W type vaccine. The vaccine has the advantages of good stability, little side effects, and high practical application value.

Description

technical field [0001] The invention relates to the field of vaccine adjuvants, in particular to a compound adjuvant, a vaccine containing the compound adjuvant and a preparation method thereof. Background technique [0002] Oil-based adjuvant vaccines are known to be effective in strengthening immunity. They are one of the most popular types of vaccines in the domestic and foreign vaccine market. There are three types of oil-containing adjuvant vaccines: water-in-oil (W / O), water-in-oil Oil (O / W) type and water-in-oil-in-water (W / O / W) type. [0003] The water-in-oil (W / O) type vaccine is the most commonly used, and the preparation method is divided into 3 steps. Firstly, Siben-85 is added to the white oil, and Tween-80 is added to the antigen aqueous solution, and then the mixture of the two is emulsified. . The significant advantage of this dosage form is that it can obtain a high level of long-lasting immune enhancement effect; the disadvantage is that it is thicker and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39
Inventor 卢宇张金秋王伟峰吕芳邓碧华侯继波
Owner JIANGSU ACAD OF AGRI SCI
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