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Compound oil adjuvant as well as preparation method and application thereof

An oil adjuvant and compound technology, applied in antiviral agents, pharmaceutical formulations, medical preparations containing active ingredients, etc., to achieve good stability, easy injection, and good immunogenicity

Active Publication Date: 2012-12-12
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above problems of complex oily adjuvant vaccine formula, poor stability and high price, the present invention provides a compound formula with simple formula, good stability and low price oil adjuvant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Application of Compound Oil Adjuvant in Porcine Foot-and-Mouth Disease Vaccine

[0025] (1) Preparation of compound oil adjuvant

[0026] Weigh 17.4g of white oil, 1.6g of oleyl alcohol polyoxyethylene ether and 1.0g of polyoxyethylene ether stearate (the mass fractions of the three are 87%, 8% and 5% respectively), and put them into 100mL clean glass bottle, shake well. After sterilizing at 115°C for 20 minutes, store at room temperature for later use.

[0027] (2) Preparation of porcine foot-and-mouth disease virus solution

[0028] In accordance with the "Compilation of Quality Standards for Veterinary Biological Quality" (2006-2008), the inactivated porcine foot-and-mouth disease antigen solution should be prepared in accordance with the relevant requirements. 7.0 LD 50 .

[0029] (3) Preparation of porcine foot-and-mouth disease vaccine

[0030] Under the condition of aseptic operation, heat the compound oil adjuvant prepared in the above (1) to...

Embodiment 2

[0035] Example 2 Application of porcine circovirus vaccine

[0036] (1) Preparation of compound oil adjuvant

[0037] a. Weigh 90.0g of white oil, 7.0g of oleyl alcohol polyoxyethylene ether and 3.0g of polyoxyethylene ether stearate (the mass fractions of the three are 90%, 7% and 3% respectively), and put them into 100mL In a clean glass bottle, shake well. After sterilizing at 115°C for 20 minutes, store at room temperature for later use.

[0038] b. Weigh 80.0g of white oil, 10.0g of oleyl alcohol polyoxyethylene ether and 10.0g of polyoxyethylene ether stearate (the mass fractions of the three are 80%, 10% and 10% respectively), and put them into 100mL In a clean glass bottle, shake well. After sterilizing at 115°C for 20 minutes, store at room temperature for later use.

[0039] c. Weigh 95.0g of white oil, 3.0g of oleyl alcohol polyoxyethylene ether and 2.0g of polyoxyethylene ether stearate (the mass fractions of the three are 95%, 3% and 2% respectively), and pu...

Embodiment 3

[0048] Example 3 Application of porcine circovirus vaccine

[0049]Take the compound oil adjuvant obtained in step a in Example 2, and prepare the porcine circovirus vaccine according to the weight ratio of 1.2:1 and 1.5:1 respectively with the inactivated porcine circovirus liquid prepared in Example 2, and name them respectively It is vaccine 5 and vaccine 6. The comparison of vaccine physical properties and immune activity is shown in Table 3.

[0050] table 3

[0051] project Vaccine 2 Vaccine 5 Vaccine 6 Exterior pale pink homogeneous emulsion pale pink homogeneous emulsion pale pink homogeneous emulsion Dosage Form W / O / W type, drop a little vaccine on the surface of clean cold water, and spread in the form of cloud W / O / W type, drop a little vaccine on the surface of clean cold water, and spread in the form of cloud W / O / W type, drop a little vaccine on the surface of clean cold water, and spread in the form of cloud centrifuga...

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PUM

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Abstract

The invention relates to the technical field of vaccine adjuvant, in particular relates to a compound oil adjuvant. The compound oil adjuvant comprises three ingredients, namely white oil, oleyl alcohol polyoxyethylene ether and polyoxyethylene ether stearate. The white oil accounts for 80-95%, the oleyl alcohol polyoxyethylene ether accounts for 1-10%, and the polyoxyethylene ether stearate accounts for 1-10%. Molecular weight of the oleyl alcohol polyoxyethylene ether is 400-600, and the molecular weight the polyoxyethylene ether stearate is 400-600. The preparation method of the compound oil adjuvant disclosed by the invention comprises the steps of respectively weighing the white oil, the oleyl alcohol polyoxyethylene ether and the polyoxyethylene ether stearate in proportion of a prescription and mixing to be uniform. The invention also discloses an application of the compound oil adjuvant in preparation of an animal vaccine. The invention successfully solves the problem that a W / O / W type emulsion vaccine is difficult to prepare, the prepared emulsion vaccine is convenient for injection and also has good immunogenicity, and stability of the prepared emulsion vaccine is better than that of the W / O / W type emulsion vaccine prepared by ISA206.

Description

technical field [0001] The invention relates to the technical field of vaccine adjuvants, in particular to a compound oil adjuvant, and also relates to a preparation method and application of the compound oil adjuvant. Background technique [0002] Oil-based adjuvant vaccines are known to be effective in strengthening immunity. They are one of the most widely used vaccines in the domestic and foreign vaccine markets. There are three types of oil-emulsion vaccines: water-in-oil (W / O) and oil-in-water. (O / W) type and water-in-oil-in-water (W / O / W) type. [0003] The water-in-oil (W / O) type is the most commonly used, and it is prepared in three steps. First, add Siben-85 to white oil, Tween-80 to an aqueous antigen solution, and then emulsify the mixture of the two. Its remarkable advantage is that it can obtain high-level and long-lasting immune enhancement effect. The disadvantage is that it is thicker and more difficult to inject. At the same time, due to the relatively ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61K39/12A61K39/135A61P31/14A61P31/20
Inventor 王金宝杜以军齐静吴家强李俊黄保华丛晓燕任素芳孙文博时建立郭立辉吕伟
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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