Surface amine functionalized rare earth doped BaFCl nanometer fluorescence labeling material and preparation method thereof

Abstract

The invention discloses a surface amine functionalized rare earth doped barium fluochloride nanometer fluorescence labeling material and a preparation method thereof, and relates to a surface functionalized water soluble nanometer fluorescence labeling material preparation. The formula of the barium fluochloride nanometer fluorescence labeling material prepared by the method of the present invention is XLn<3+>-(1-x)BaFCl (Ln=Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb; X=0-60 mol% ). According to the preparation method, polypropylenimine is adopted as a surfactant, and a one-step reaction is performed to prepare the surface amine functionalized water soluble rare earth doped barium fluochloride nanometer fluorescence labeling material, wherein the results of transmission electron microscope, infrared absorption spectrum, gravitational thermal analysis and other test methods show that the polypropylenimine is effectively coated on the surfaces of the nanoparticles. The prepared nanometer powder has good solubility; with the amino groups on the surface, the basis is established for the further combination of the biological molecules; with the rare earth ion doping, the material can achieve the purposes of strong luminous intensity, and rare earth ion fluorescence emission with long fluorescence lifetime.

Description

technical field [0001] The invention relates to a water-soluble surface amino-functionalized nano-fluorescence marking material and a preparation method thereof, in particular to a surface-amino-functionalized water-soluble rare earth-doped BaFCl nano-fluorescence marking material and a preparation method thereof. Background technique [0002] Rare earth-doped nanoluminescent materials, due to their stable photophysical and photochemical properties, large Stokes shift, and low toxicity, have received more and more attention in the fields of bio-optical imaging and fluorescent immunoassays. more and more attention. Nanomaterials used as bioluminescent probes need to have good water solubility, and their surfaces need to have functional groups that can bind biomolecules, such as carboxyl, amino, and sulfhydryl groups. However, the nanoluminescent materials obtained by traditional organic phase preparation methods are insoluble in water and lack surface functional groups that ...

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Problems solved by technology

However, the nano-luminescent materials obtained by traditional organic phase preparation methods are insoluble in water and lack surface functional groups that can bind to biomolecules.

Although subsequent surface treatment methods, such as silica coating, layer by layer and ligand exchange, can modify the surface of nanomaterials, so that nanoparticles can be dissolved in water and can connect biomolecules, but However, there are many limitations in the application of these methods

The method of silica coating is complex in operation, poor in repeatability, and easily leads to particle agglomeration; layer-by-layer adsorption is not selective for biomolecules, resulting in confusion of connected biomolecules, and the connected biomolecules are also easy to dissociate; ligands The exchange needs to be carried out under the protection of high temperature and inert atmosphere, and the experimental conditions are relatively harsh.

However, the preparation of water-soluble barium fluoride nanomaterials with surface amino functionalization has not been reported at home and abroad.

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