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Gold-coated polystyrene nanoparticles and preparation method

A technology of polystyrene nanometer and styrene monomer, which is applied in the field of medical immunology in vitro diagnosis, to achieve the effect of improving measurement sensitivity and good use effect

Active Publication Date: 2012-06-20
安徽信灵检验医学科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the sensitivity problem of immunoturbidimetry determination, the present invention provides a polystyrene nano-gold-coated particle and a preparation method thereof, due to the rough gold surface of the particle, non-specific physical adsorption of antibodies or anti-antibodies in the range of isoelectric point , forming specific antibodies or antigen-adsorbed gold-coated microsomes, that is, immune-enhancing in vitro diagnostic reagents

Method used

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  • Gold-coated polystyrene nanoparticles and preparation method

Examples

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Effect test

example 1

[0079] [Example 1] Latex agglutination test

[0080] Although latex particles have good adsorption performance for protein and other biopolymers (but it is incomparable with the adsorption of gold surface), it can be used as a carrier to adsorb certain antigens (or antibodies). Under suitable conditions, namely Agglomeration of particles can occur, this is a latex agglutination test. If the latex particles are too large, the stability in the dilute concentration suspension is poor, the surface area of ​​the particles is large, and certain antigens (especially antibodies) cannot be quantitatively adsorbed, and only qualitative or semi-quantitative tests can be done.

example 2

[0081] [Example 2] Colloidal gold marking technology test

[0082] Colloidal gold is chloroauric acid polymerized into gold particles of a certain size under the action of a reducing agent to form a negatively charged hydrophobic colloidal solution (colloidal gold has strong surface adsorption but poor colloidal stability). Utilizing its negatively charged nature in an alkaline environment, it forms a firm bond with the positive charge range of protein molecules by electrostatic attraction to form a gold-labeled reagent. Using nitrocellulose membrane as a carrier, drop reagents and specimens onto the membrane Above, the reaction is gradually performed by diafiltration, and the positive result shows red spots on the membrane, which is a qualitative test.

[0083] In order to further verify the polystyrene nano-gold-coated particles of the present invention, the following two tests were carried out:

[0084] 【Test 1】Gold-coated particle size test

[0085] Centrifuge the synthesized gol...

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Abstract

The invention relates to gold-coated polystyrene nanoparticles and preparation method thereof. The Gold-coated polystyrene nanoparticles contain styrene monomer (purified), a camellia oil emulsifier, a gold chloride crosslinking agent, polyethylene glycol 6000, polyether triol, sodium periodate, sodium hydroxide, white phosphorus-ether saturated solution, and distilled water. The preparation method of the gold-coated polystyrene nanoparticles comprises the following steps: styrene monomer purification, camellia oil emulsifier preparation, gold chloride crosslinking agent preparation, styrene monomer polymerization, crosslinking of polystyrene particles and gold chloride crosslinking agent, and size screening of gold-coated nanoparticles. The gold-coated polystyrene nanoparticles prepared by the invention can participate in immunoturbidimetry as antigen or antibody carrier to amplify the sensitivity for determining a target antigen or antibody by geometric progression so as to achievedirect determination of microgram-scale or nanoscale target antigens or antibodies which can be determined only by complex chemiluminescence and enzyme-linked immunoassay method through a biochemical analyzer, thereby ensuring good actual use effect.

Description

Technical field [0001] The present invention relates to the field of medical immunological in vitro diagnostics, in particular to the immunoturbidimetric method used in in vitro diagnostic reagents for medical devices as a non-specific carrier for antigens or antibodies (proteins and other biopolymers) involved in the reaction and a preparation method thereof . Background technique [0002] The basic principle of immunoturbidimetry is to detect the amount of light passing through a certain volume of solution. When light passes through, due to the reflection and absorption of light by the antigen-antibody complex in the test solution, the transmitted light decreases, and the measured luminous flux is inversely proportional to the amount of antigen-antibody complex. The existing in vitro diagnostic reagent immunoturbidimetric method is: after the immunized animal (such as sheep or rabbit) for the determination of the target antigen or antibody produces antibody or anti-antibody (p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/553G01N33/531
Inventor 王之侃
Owner 安徽信灵检验医学科技股份有限公司
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