Metal/nano metal based solid glucose electrode and its preparation method

A nano-metal and metal nano-technology, applied in the field of electrochemical detection, can solve the problems that the sensitivity and life of conductive polymer biosensors cannot meet the needs of actual use, biosensors cannot, and limit the scope of application, etc., to achieve long service life and detection The effect of low lower limit and compact size

Inactive Publication Date: 2012-05-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) It is difficult for electrochemically active conductive polymers (especially polyaniline) to eliminate the interference of electroactive substances on ampere biosensors (when used at higher potentials)
[0006] (2) Usually, electrochemically active conductive polymers have large background currents at low potentials, so biosensors directly prepared from them cannot operate at negative potentials, which limits their applicability in biosensor preparation scope
[0007] (3) The sensitivity and lifetime of some conductive polymer biosensors cannot meet the needs of actual use

Method used

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  • Metal/nano metal based solid glucose electrode and its preparation method
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  • Metal/nano metal based solid glucose electrode and its preparation method

Examples

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preparation example Construction

[0026] The steps of the preparation method of the metal / nanometal-based solid glucose electrode are as follows:

[0027] 1) For the first metal wire 1 with a length of 5-15 cm, first remove the surface oil stains with acetone solution, then wash with 1-2M dilute hydrochloric acid or dilute nitric acid to remove oxides, rinse with deionized water and then dry;

[0028] 2) The positive pole or negative pole of the output voltage of the function signal generator 8 is connected to the negative pole or positive pole of the diode 7, and the other pole of the diode 7 is connected to the signal channel of the oscilloscope. Adjust the function signal generator 8, observe the oscilloscope graph, and obtain The power supply is a half-sine wave carrier DC power supply with a peak value of 450-800mV and a frequency of 50HZ;

[0029] 3) Connect one end of the first wire 1 to the negative electrode of the output voltage of the function signal generator 8 through a wire, connect one end of th...

Embodiment 1

[0035] 1) The surface of the first metal wire 1 with a length of 5 cm is polished with corundum powder, then washed with 1M dilute hydrochloric acid or dilute nitric acid, rinsed with deionized water and dried;

[0036] 2) Adjust the function signal generator 8 (Rite Electronics SG1005A) to generate a sine wave function signal with a peak value of 600 mV and a frequency of 50Hz; then connect the negative pole of the output voltage of the function signal generator 8 (Rite Electronics SG1005A) with the The cathode of diode 7 (Schottky diode MBR1045) is connected to the cathode, and the anode of diode 7 (Schottky diode MBR1045) is connected to the signal channel of the oscilloscope (Tektronix TDS1002). Observe the oscilloscope graph and show that the obtained power supply is 450mV in peak value. Half-sine wave carrier DC power supply with a frequency of 50HZ;

[0037] 3) Connect one end of the polished and cleaned first wire 1 to the anode of the diode 7 (Schottky diode MBR1045) ...

Embodiment 2

[0042] 1) The surface of the first metal wire 1 with a length of 15 cm is polished with corundum powder, then washed with 2M dilute hydrochloric acid or dilute nitric acid, rinsed with deionized water and dried;

[0043] 2) Adjust the function signal generator 8 (Rite Electronics SG1005A) to generate a sine wave function signal with a peak value of 1000mV and a frequency of 50Hz; then output the voltage at the positive pole and diode of the function signal generator 8 (Rite Electronics SG1005A). The anode of 7 (Schottky diode MBR1045) is connected, and the cathode of diode 7 (Schottky diode MBR1045) is connected with the signal channel of the oscilloscope (Tektronix TDS1002). It is a half-sine wave carrier DC power supply of 50HZ;

[0044] 3) Connect one end of the polished and cleaned first wire 1 to the negative electrode of the output voltage of the function signal generator 8 (Rite Electronics SG1005A) through a wire, and connect one end of the second wire 5 to the diode 7...

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Abstract

The invention discloses a metal/nano metal based solid glucose electrode and its preparation method. The metal/nano metal based solid glucose electrode possesses a first metal wire, a nanoparticle grade metal nano layer is coated on the surface at the lower part of the first metal wire, a polyacrylonitrile (PAN for short) porous membrane is coated on external surface of the metal nano layer, glucose oxidase (GOD) is carried on the polyacrylonitrile membrane. The metal is Ag, Au, Pt or other precious metals. The solid glucose electrode has the advantages of small volume, fast detection response, high sensitivity, low detection lower limit, long service life and the like, and is suitable for detecting blood sugar concentration and glucose content of the fruit juice, beverage, fruit and the like.

Description

technical field [0001] The invention relates to electrochemical detection technology, in particular to a metal / nano metal-based solid glucose electrode and a preparation method thereof. Background technique [0002] Biosensors are a new technology developed in the late 1960s. Its advantages are good specificity, high sensitivity, fast measurement, simple operation, low measurement cost, and continuous online, in-situ and in-body monitoring can be realized. As a means of biochemical detection, biosensors have shown an increasingly important role in the fields of biomedical engineering, clinical diagnosis, environmental monitoring, food hygiene and scientific research. [0003] The biologically active substances in biosensors are generally soluble in water, which are inherently unstable and need to be fixed on various carriers to prolong their activity. At present, the most widely used immobilized carrier is the polymer material. Polymer biosensors mainly use polymers as ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30C12Q1/54C12Q1/26
Inventor 郑豪丁茜叶瑛潘依雯陈雪刚夏枚生张海燕
Owner ZHEJIANG UNIV
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