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Preparation method of injectable polypeptide hydrogel

A technology of hydrogel and aqueous solution, applied in the field of preparation of injectable polypeptide hydrogel, can solve the problems of increasing cost and reducing application effect, and achieve the effect of promoting formation, good biocompatibility and good biocompatibility

Inactive Publication Date: 2012-04-11
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If the time control is not good, it will greatly reduce its application effect
On the other hand, many different applications require different strengths of hydrogels. Simply increasing the concentration of peptides to increase the strength of hydrogels will increase the cost and bring some negative effects.

Method used

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  • Preparation method of injectable polypeptide hydrogel
  • Preparation method of injectable polypeptide hydrogel
  • Preparation method of injectable polypeptide hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Utilize solid-phase synthesis method, synthesize the polypeptide molecule EMK16-II of ion complementary type (sequence is (MEMEMKMK) 2 , where M represents methionine, which is a hydrophobic amino acid, E represents glutamic acid, which is a negatively charged hydrophilic amino acid, K represents lysine, which is a positively charged hydrophilic amino acid, and they are arranged alternately to form a Amphiphilic polypeptide molecules).

[0020] 2. The obtained polypeptide was dissolved in pure water, and freeze-dried to remove residual impurities during the synthesis process, thereby obtaining a solid sample powder.

[0021] 3. Dissolve the sample powder in sodium chloride solution (sodium chloride concentration is 3mM) and undergo ultrasonic treatment for 10 minutes (100W, 40,000Hz) until the sample is completely dispersed and dissolved. The concentration of EMK16-II in the final solution is 5mg / mL.

[0022] The above solution was left to stand for 1 hour to obta...

Embodiment 2

[0024] The concentration of EMK16-II was 30 mg / mL, and the rest of the experimental conditions were the same as in Example 1.

[0025] The above solution can be left to stand for 2 minutes to obtain a hydrogel material that can be injected into a gel. The inversion test proves that it is a solid state that does not flow; it is sucked into the syringe and then injected onto the surface of a vertically placed petri dish, and it instantly returns to a solid state. Hydrogel; the storage modulus is around 1000 Pascal; after more than 50 times of mechanical destruction and recovery experiments, its mechanical properties have no obvious change.

Embodiment 3

[0027] The concentration of sodium chloride added was 20 mM, and the rest of the experimental conditions were the same as in Example 1.

[0028] The above solution can be left to stand for 5 minutes to obtain a hydrogel material that can be injected into a gel. The inversion test proves that it is a solid state that does not flow; it is sucked into the syringe and injected onto the surface of a vertically placed petri dish, and it instantly returns to a solid state. Hydrogel; but the storage modulus of the gel is low, about 50 Pascals; after repeated mechanical damage 10 times, the storage modulus drops by about 30%.

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Abstract

The invention relates to a preparation method of injectable polypeptide hydrogel. The preparation method comprises the steps of: dissolving ion compensation polypeptide in 3-20 mM MX aqueous solution under assistance of ultrasonic vibration, and carrying out self-assembly on the obtained solution to obtain the injectable polypeptide hydrogel, wherein the ion compensation polypeptide chain is composed of alternately arranged hydrophobic and hydrophilic amino acids, the hydrophilic amino acid is in charge periodic complementation arrangement, the hydrophobic amino acid is methionine, in the ioncompensation polypeptide aqueous solution, the concentration of the ion compensation polypeptide is 5-30 mg / mL, M is Na or K, and X is Cl, Br or I. The obtained hydrogel has good biocompatibility anddegradability, can be changed into fluid under the action of mechanical force, can rapidly restore after mechanical damage, is especially suitable for injection by an injector, and is convenient for use.

Description

technical field [0001] The invention relates to a preparation method of injectable polypeptide hydrogel. Background technique [0002] In recent years, nano-microstructure biomaterials based on the self-assembly of short peptides have been widely used in nanotechnology, surface engineering and biomedical technology fields. Especially ion-complementary polypeptides, such polypeptide chains are composed of alternately arranged hydrophobic and hydrophilic amino acids, and have special amphipathic properties. In particular, the hydrophilic amino acids on the polypeptide chain present a special periodic complementary arrangement of charges, which enables this type of ion-compensating polypeptide to quickly self-assemble into a controllable biological nanofibrous structure, and then form a hydrogel with a water content of more than 95%. glue. Due to their good biocompatibility and degradability, this emerging hydrogel has extremely important applications in bioengineering fields...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/00A61L31/04A61L31/14A61L27/22A61L27/52A61K47/42A61K9/06
Inventor 王炜邹大维秦猛曹毅
Owner NANJING UNIV
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