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Preparation method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle

A technology of hydroxypropyltrimethylammonium chloride and carboxymethyl chitosan, which is applied in the field of preparation of chitosan nanoparticles, can solve the problem of harsh process conditions, difficult cleaning of cross-linking agents or emulsifiers, and cumbersome operations and other problems, to achieve the effect of simple preparation process, relatively low cost and simple operation

Active Publication Date: 2012-03-21
黑龙江凯正利华生物化工科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the existing problems in the preparation of chitosan nanoparticles that the process conditions are harsh, the operation is cumbersome, the preparation process needs to add a cross-linking agent or emulsifier, and the added cross-linking agent or emulsifier is not easy to clean. , and provide the preparation method of N-2-hydroxypropyltrimethylammonium chloride chitosan / N, O-carboxymethyl chitosan nanoparticles

Method used

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  • Preparation method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle
  • Preparation method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle
  • Preparation method of N-2-hydroxypropyl trimethyl ammonium chloride chitosan/ N,O-carboxymethyl chitosan naonparticle

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specific Embodiment approach 1

[0018] Specific embodiment one: present embodiment N-2-hydroxypropyltrimethylammonium chloride chitosan / N, the preparation method of O-carboxymethyl chitosan nanoparticle, realizes according to the following steps:

[0019] 1. Put 5g of powdered chitosan in a 250mL three-neck flask, add 40mL of isopropanol and stir, soak for 1 to 12 hours, then add 60mL of NaOH solution with a concentration of 10 to 40mol / L, stir well and soak for 2 to 24 hours, Obtain alkalized chitosan;

[0020] 2. Heat 20g of chloroacetic acid in a water bath and dissolve it in 10mL of isopropanol, add it to the alkalized chitosan in 5 times under stirring, with an interval of 10 minutes each time, and then react at 40-80°C for 2-8 hours. Prepare the carboxymethyl chitosan mixture, then add 20mL of distilled water, adjust the pH to 7.0 with hydrochloric acid with a volume concentration of 1%, then suction filter with a Buchner funnel, add 4 times the amount of absolute ethanol to the filtrate to fully preci...

Embodiment 1

[0032] N-2-hydroxypropyltrimethylammonium chloride chitosan / N, the preparation method of O-carboxymethyl chitosan nanoparticle, realizes according to the following steps:

[0033] 1. Put 5g of powdered chitosan in a 250mL three-neck flask, add 40mL of isopropanol and stir, soak for 2 hours, add 60mL of NaOH solution with a concentration of 30mol / L, stir well and soak for 2 hours to obtain alkalized chitosan ;

[0034] 2. Heat 20g of chloroacetic acid in a water bath and dissolve it in 10mL of isopropanol, add it to the alkalized chitosan in 5 times under stirring, with an interval of 10min each time, and then react at 70°C for 8h to obtain carboxymethyl base chitosan mixture, then add 20mL of distilled water, adjust the pH to 7.0 with hydrochloric acid with a volume concentration of 1%, and then filter with Buchner funnel, add 4 times the amount of absolute ethanol to the filtrate to fully precipitate, filter, and then use Wash with ethanol and absolute ethanol with a volume ...

specific Embodiment approach 2

[0041] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, after soaking for 4 hours, add 60 mL of NaOH solution with a concentration of 20 mol / L, stir well and then soak for 5 hours. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

A preparation method of an N-2-hydroxypropyl trimethyl ammonium chloride chitosan / N,O-carboxymethyl chitosan naonparticle relates to a preparation method of the chitosan naonparticle. The method comprises the following steps of: 1, taking an N-2-hydroxypropyl trimethyl ammonium chloride chitosan solution, dripping an N,O-carboxymethyl chitosan naonparticle solution therein, stirring magnetically at a room temperature and under a sterile condition, and obtaining a solution A; and 2, centrifugating the solution A, eluting the deposit with deionized water for three times and performing freeze drying under vacuum to obtain the N-2-hydroxypropyl trimethyl ammonium chloride chitosan / N,O-carboxymethyl chitosan naonparticle. Compared with the chitosan naonparticle, the N-2-hydroxypropyl trimethyl ammonium chloride chitosan / N,O-carboxymethyl chitosan naonparticle obtained by the method has the advantages that the particle diameters can be easily controlled and are uniformly distributed, the biological compatibility is good, the stability is high, and has the characteristics of good water solubility, electropositive property and the like; in the process of preparing the nanoparticle, the safety and the nontoxicity are achieved; the preparation process is simple; the operation is simple; the preparation conditions are mild; the cost is low; and the N-2-hydroxypropyl trimethyl ammonium chloride chitosan / N,O-carboxymethyl chitosan naonparticle can be easily produced on a large scale.

Description

technical field [0001] The invention relates to a preparation method of chitosan nanoparticles. Background technique [0002] Nanoparticles of biodegradable polymer materials are new forms of drug carriers emerging with the rapid development of nanotechnology in recent years. The particle size of nanoparticles is usually 1-1000nm, which is a kind of solid colloidal particles composed of polymer substances, which can be dispersed in water to form a similar colloidal solution. [0003] There are many methods for preparing nanoparticles, mainly including emulsification solvent evaporation method, covalent cross-linking method, ion cross-linking method, complex coacervation method, polyelectrolyte composite method, precipitation and precipitation method, etc. The polymer materials commonly used to prepare nanoparticles mainly include chitosan and its derivatives, alginic acid and its salts, gelatin, methyl cellulose, ethyl cellulose, poly D, L-lactide, polylactic acid ( PLA), ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/14C08L5/08C08B37/08
Inventor 赵凯金政张旭陈刚吴昊
Owner 黑龙江凯正利华生物化工科技有限公司
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