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Inactivated vaccine for infectious coryza of chickens

A technology for chicken infectious rhinitis and inactivated vaccine, which is applied in the directions of antibacterial drugs, antibody medical ingredients, and medical preparations containing active ingredients, etc., and can solve the problems of short duration, side reactions, and low antibody titers

Inactive Publication Date: 2012-03-21
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The oil emulsion inactivated vaccine antibody lasts longer, but there are different degrees of side effects
The inactivated vaccine with aluminum gel adjuvant has no side effects, but the antibody titer is low and the duration is short

Method used

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  • Inactivated vaccine for infectious coryza of chickens
  • Inactivated vaccine for infectious coryza of chickens
  • Inactivated vaccine for infectious coryza of chickens

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0016] (3) Preparation of seedling liquid

[0017] 1) Bacterial liquid culture Add 100ml of seed liquid into 4000ml of semi-synthetic medium, cultivate at 37°C for 18-20 hours, vibrate the culture bottle twice, and confirm that there is no bacterial contamination by pure picking, add 0.05% formaldehyde solution, place Store at 2-8°C.

[0018] 2) Concentration and inactivation of the bacterium liquid for making vaccines Use a hollow fiber ultrafilter to concentrate the purely qualified bacterium liquid, and then make a suspension with PBS of pH 7.2, so that every 1ml contains at least 5 billion bacterium (using biological The product national standard product "China Bacterial Turbidity Standard" is compared, set standard tube containing Haemophilus paragallinarum to be 2,800,000,000 / ml), then add 0.15% formaldehyde solution and 0.01% thimerosal by volume ratio, at 2~8 ℃ Sterilized for 7 days, the aseptic growth was tested as the vaccine antigen.

[0019] (4) Preparation of ad...

Embodiment 1

[0038] 1 strain

[0039] The strain used for manufacturing and inspection is Haemophilus paragallinarum type A C-Hpg-8 strain, which is identified, kept and supplied by China Veterinary Drug Control Institute.

[0040] 2 Vaccine manufacturing and inspection of semi-finished products

[0041] (1) Preparation of seeds for production

[0042] 1) Propagation and identification of primary seeds Take the strains and inoculate them on the chicken broth agar 2 After culturing at 37°C for 16-18 hours in the environment, select several typical bacterial lawns (colonies) with strong fluorescence and inoculate them in the yolk sac of 5-day-old chicken embryos, continue to incubate at 37°C, and collect the yolks of chicken embryos that died within 30 hours The liquid, after passing the pure inspection, is regarded as a first-grade seed. Storage below -20°C should not exceed 1 month, otherwise it will be rejuvenated through chicken embryos, but it should not exceed 6 generations.

[004...

Embodiment 2

[0058] According to " Chicken Infectious Rhinitis Inactivated Vaccine Manufacture and Inspection Regulations " (Ministry of Agriculture of the People's Republic of China. The People's Republic of China Veterinary Biological Products Regulations 2000 Edition. Chemical Industry Press, 2000, the present invention is hereinafter referred to as " Regulations ") Test the vaccine according to the test method above.

[0059] 1. Sterility test According to the "Procedures", aseptic growth.

[0060] 2. Safety inspection Use 8 healthy susceptible chickens aged 2-3 months, inject 1ml of vaccine subcutaneously into each chicken, observe for 14 days, there should be no abnormal reaction.

[0061] 3. Efficacy test Eight healthy susceptible chickens aged 2 to 3 months were used, and each chicken was subcutaneously injected with 0.5ml of the vaccine. One month later, together with 4 control chickens with the same conditions, each infraorbital sinus was injected with 0.2ml of a 16-hour culture...

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Abstract

The invention relates to an inactivated vaccine for infectious coryza of chickens. The preparation method of the inactivated vaccine for infectious coryza of chickens comprises the following steps: using sterilized PBS (phosphate-buffered saline) to dilute inactivated haemophilus paragallinarum A type C-hpg-8 strain concentrated bacteria liquid prepared by the conventional method by a certain proportion, then mixing with vaccine adjuvants taking carbomer as a main component according to the proportion, stirring and shaking up so as to prepare the inactivated vaccine for infectious coryza of chickens. After a chicken is immunized by the inactivated vaccine for infectious coryza of chickens, the following advantages can be realized: the antibody titer generated by the inoculated chicken is high, the protection period of the antibody is long, and the protection rate against strong virus is high and the like.

Description

technical field [0001] The invention relates to an inactivated chicken infectious rhinitis vaccine, belonging to the field of biology, in particular to the field of veterinary biological products. Background technique [0002] There are mainly two types of rhinitis inactivated vaccines currently popular in the market, including oil adjuvant vaccines and aluminum gel adjuvant vaccines. The oil emulsion inactivated vaccine antibody lasted longer, but there were side effects in different degrees. The inactivated vaccine with aluminum gel adjuvant has no side effects, but the antibody titer is low and the duration is short. [0003] The inactivated chicken infectious rhinitis vaccine prepared by the novel adjuvant of the present invention has the advantages of high safety, no side effects, rapid antibody production and high protection rate, and is not only suitable for routine immunization of chickens, but also effectively prevents the occurrence of epidemic diseases. And it i...

Claims

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Application Information

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IPC IPC(8): A61K39/102A61K39/39A61P31/04
Inventor 韩建文周忠号李平杜元钊
Owner YEBIO BIOENG OF QINGDAO
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