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Detection method of sodion and kit

A sodium ion and kit technology, applied in the field of sodium ion detection, can solve problems such as inability to accurately judge the reaction end point, inability to obtain opacity, and error in test results, so as to avoid errors, prevent large fluctuations in pH value, and improve The effect of dispersion performance

Active Publication Date: 2013-11-27
CHANGCHUN HUILI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reaction time between sodium ions and 6-antimony potassium hydroxide is too long, and the end point of the reaction cannot be accurately judged, so errors will occur in the results
In addition, the small particle precipitation produced by the reaction of sodium ions and 6-antimony potassium hydroxide is also easy to aggregate into crystals of different sizes, which cannot obtain uniform turbidity, and will also cause large errors in the detection results

Method used

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  • Detection method of sodion and kit
  • Detection method of sodion and kit
  • Detection method of sodion and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: method of the present invention

[0028] Mix agar gel and 6-antimony potassium hydroxide (agar gel concentration is 250mg / L, 6-antimony potassium hydroxide concentration is 14g / L), in 100mmol / L Tris, 30mmol / L Tris- In the potassium dihydrogen phosphate buffer system of HCl, 26mmol / L dipotassium hydrogen phosphate, 4mmol / L, with test sample and compound alcohol (95% ethanol and 5% ethylene glycol) by volume ratio, test sample: Mixed solution: complex alcohol = 1:15:10 ratio reaction, the resulting suspension was detected at a wavelength of 630nm for absorbance A 样 , and then with the A 标 Turbidity, calculate the sodium ion concentration of the sample to be tested, the formula is sample sodium ion concentration (mmol / L)=standard solution concentration (140mmol / L)×A 样 / A 标 .

Embodiment 2

[0029] Embodiment 2: method of the present invention

[0030] Gum Arabic and 6-antimony potassium hydroxide are used to form a mixed solution (the concentration of gum arabic is 1250mg / L, and the concentration of 6-antimony potassium hydroxide is 15g / L). In the potassium dihydrogen phosphate buffer system of HCl, 23mmol / L dipotassium hydrogen phosphate, 3mmol / L, with test sample and compound alcohol (95% ethanol and 5% Virahol) by volume ratio, test sample: Mixed solution: complex alcohol = 1:15:10 ratio reaction, the obtained suspension is detected at a wavelength of 630nm for absorbance A 样 , and then with the A 标 Turbidity, calculate the sodium ion concentration of the sample to be tested, the formula is sample sodium ion concentration (mmol / L)=standard solution concentration (140mmol / L)×A 样 / A 标 .

Embodiment 3

[0031] Embodiment 3: method of the present invention

[0032] Polyethylene glycol 20000 and 6-antimony potassium hydroxide form a mixed solution (polyethylene glycol 20000 concentration is 7.5g / L, 6-antimony potassium hydroxide concentration is 12g / L), at pH7.5 80mmol / L In Tris, 20mmol / LTris-HCl, 20mmol / L dipotassium hydrogen phosphate, potassium dihydrogen phosphate buffer system of 5mmol / L, with test sample and complex alcohol (95% ethanol, 3% ethylene glycol and 2% isopropanol) by volume ratio, sample to be tested: mixed solution: compound alcohol=1: 15: 10 ratio reaction, the suspension liquid that obtains detects absorbance A under 630nm wavelength 样 , and then with the A 标 Turbidity, calculate the sodium ion concentration of the sample to be tested, the formula is sample sodium ion concentration (mmol / L)=standard solution concentration (140mmol / L)×A 样 / A 标 .

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Abstract

The invention relates to the field of biochemistry, and discloses a detection method of sodion and a kit. The method provided by the invention comprises the following steps of: mixing one component of agaropectin, Arabic gum or polyethylene glycol 2000 with 6-antimony potassium hydroxide to form a mixed liquor, allowing the mixed liquor in a buffer system of pH 7.2-7.5 to react with a sample to be detected and mixtalol to obtain a 6-sodium antimonite hydroxide suspending liquid, detecting absorbance at a 630nm wavelength, performing turbidimetry with a standard solution which undergoes the above same processing, and calculating the sodion concentration of the sample to be detected. The kit provided by the invention comprises one component of 200-300mg / L of agaropectin, 1000-1500mg / L of Arabic gum or 5-10g / L of polyethylene glycol 20000, 12-15g / L of 6-antimony potassium hydroxide and mixtalol. The method provided by the invention enables rapid precipitation of the reaction between Na+ and 6-antimony potassium hydroxide, avoids precipitation polymerization, raises the accuracy of the detection, and can be widely used to detect the sodion concentration.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a method and a kit for detecting sodium ions. Background technique [0002] An electrolyte is a compound that can conduct electricity in an aqueous solution or in a molten state. In the human body, electrolytes are widely distributed in intracellular and extracellular fluids. The main cations in the electrolytes of the extracellular fluids are sodium ions, and the main anions are chloride ions and bicarbonate ions; the main cations in the electrolytes of the intracellular fluids are It is potassium ion and magnesium ion, and the main anion is hydrogen phosphate ion. These electrolyte ions form a dynamic balance with water through the regulation of nerves and body fluids. Both of them participate in the body's metabolic activities and other important functions of the body, and play a key role in the maintenance of normal life activities. [0003] The imbalance between electrolyte ion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 董理
Owner CHANGCHUN HUILI BIOTECH CO LTD
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