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A kind of extraction method of colchicine

A colchicine and extraction method technology, which is applied in the field of colchicine extraction, can solve the problems of low purity, low extraction rate, incomplete colchicine extraction, etc., and achieve the effect of economical use and improved extraction rate

Active Publication Date: 2011-12-14
YUNNAN XISHUANGBANNA BANNA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The extraction of colchicine by the original method has shortcomings such as incomplete extraction, low extraction rate, and low purity.

Method used

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  • A kind of extraction method of colchicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Extraction of colchicine:

[0025] 1. Pre-treatment: Select Lijiang Arrowhead Mushrooms that have passed the inspection to remove impurities and foreign matter, and then rinse them with water to remove the sediment.

[0026] 2. Drying: Dry the rinsed Lijiang Arrowhead Mushroom in a hot air circulation oven to remove moisture.

[0027] 3. Reflux extraction: The dried Lijiang Arrowhead mushroom was reflux extracted three times with 90% (volume concentration, the same below) ethanol, 2 hours each time.

[0028] 4. Ethanol recovery: Concentrate the reflux extract to recover ethanol.

[0029] 5. Water precipitation: the concentrated solution is mixed with water (the amount of water added is 1 times the volume of the concentrated solution), and the pH is adjusted to 1 with sulfuric acid, placed, filtered, and the supernatant is taken.

[0030] 6. Elution: The supernatant was eluted with a mixed ion exchange resin. After the elution was completed, the elution column wa...

Embodiment 2

[0037] Extraction of colchicine:

[0038] 1. Pre-treatment: Select Lijiang Arrowhead Mushrooms that have passed the inspection to remove impurities and foreign matter, and then rinse them with water to remove the sediment.

[0039] 2. Drying: Dry the rinsed Lijiang Arrowhead Mushroom in a hot air circulation oven to remove moisture.

[0040] 3. Reflux extraction: The dried Lijiang Arrowhead mushroom was reflux extracted three times with 90% ethanol, 2 hours each time.

[0041] 4. Ethanol recovery: Concentrate the reflux extract to recover ethanol.

[0042] 5. Water precipitation: add water to the concentrated solution (the amount of water added is 3 times the volume of the concentrated solution), mix, and adjust the pH to 3 with sulfuric acid, let it stand, filter, and take the supernatant.

[0043] 6. Elution: The supernatant is eluted with a mixed ion exchange resin. After the elution is completed, the elution column is basified with ammonia water to adjust the pH to...

Embodiment 3

[0050] Extraction of colchicine:

[0051] 1. Pre-treatment: Select Lijiang Arrowhead Mushrooms that have passed the inspection to remove impurities and foreign matter, and then rinse them with water to remove the sediment.

[0052] 2. Drying: Dry the rinsed Lijiang Arrowhead Mushroom in a hot air circulation oven to remove moisture.

[0053] 3. Reflux extraction: The dried Lijiang Arrowhead mushroom was reflux extracted three times with 90% ethanol, 2 hours each time.

[0054] 4. Ethanol recovery: Concentrate the reflux extract to recover ethanol.

[0055] 5. Water precipitation: add water to the concentrated solution (the amount of water added is twice the volume of the concentrated solution), mix, and adjust the pH to 2 with sulfuric acid, let it stand, filter, and take the supernatant.

[0056] 6. Elution: The supernatant was eluted with a mixed ion exchange resin. After the elution was completed, the elution column was basified with ammonia water to adjust the pH t...

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Abstract

The invention discloses an extraction method of colchicine. The extraction method comprises the following steps of 1, pretreating iphigenia indica A. Gray, 2, drying, 3, carrying out backflow extraction, 4, recovering ethanol, 5, adding water, precipitating and collecting supernatant, 6, carrying out elution of the supernatant, adjusting a pH value to a pH value of 9 to 13, alkalifying and carrying out elution again, 7, drying to remove methanol, 8, adding sulfuric acid to dissolve and filtering, 9, extracting, filtering, precipitating and drying in a vacuum, and 10, recrystallizing, filtering, precipitating, drying in a vacuum, and crushing produced crystals to obtain refined colchicine products. The extraction method improves an extraction rate of colchicine (wherein the extraction rate is increased by 20%), enables dissociated alkaloids or alkaloid salts to be dissolved out effectively thereby improving colchicine purity, and reduces use of organic solvents.

Description

[0001] technical field [0002] The invention relates to a method for extracting medicines, in particular to a method for extracting colchicine. Background technique [0003] Colchicine is an alkaloid originally extracted from the liliaceous plant Colchicum autumnale. Pure colchicine is in the form of yellow needle-like crystals with a melting point of 157°C. Soluble in water, ethanol and chloroform. Bitter, poisonous. Since American scholar Blakeslee (A.F.Blakeslee) succeeded in doubling the chromosome number of Datura and other plants with colchicine in 1937, colchicine has been widely used in cytology, genetics research and plant breeding. at work. Colchicine is clinically used to treat acute attacks of gouty arthritis and prevent acute attacks of recurrent gouty arthritis. [0004] The original method for extracting colchicine has deficiencies such as incomplete extraction, low extraction rate, and low purity. Contents of the invention [0005] The purpose of ...

Claims

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Application Information

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IPC IPC(8): C07C233/32C07C231/24
Inventor 杨雄金兆元李维
Owner YUNNAN XISHUANGBANNA BANNA PHARMA
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