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Magnetic fluorescent bifunctional nano biological probe and preparation method thereof

A nano-biological probe and fluorescent nano-particle technology, which is applied in the fields of polymer materials and medical engineering, can solve the problems of poor biocompatibility, low photostability, and large side effects in vivo, and achieve good biocompatibility. , Improve the sensitivity and accuracy, the effect of high photostability

Inactive Publication Date: 2011-07-20
苏州同科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a magnetic fluorescent dual-functional nano-biological probe and its preparation method. The nano-probe can be used for multi-mode imaging analysis of cells, which solves the problem of complexing magnetic fluorescent materials with cells and entering animals in the prior art. Poor biocompatibility, weakened magnetism, low photostability, fluorescence quenching, large side effects in vivo, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of Py-PEG:

[0028] 3mmol of 1-pyrenebutyric acid and 3mmol of polyethylene glycol monomethyl ether (MPEG) with a molecular weight of 2000, 60mL of chloroform, 7mmol of 1,3-dicyclohexylcarbodiimide (DCC), 3mmol of 4-bis Methylaminopyridine (DMAP) and 7 mmol triethylamine (TEA) were reacted at room temperature for 36 hours under the protection of nitrogen. After the reaction was completed, the by-product (dicyclohexyl urea) was removed by filtration, and the solvent was removed under reduced pressure and dissolved in dichloromethane. The reaction product is precipitated in excess ether or petroleum ether, and the product after filtration and purification is the polymer Py-PEG containing fluorescent functional groups;

[0029] (2) Preparation of magnetic fluorescent nanoparticles (FMNPs):

[0030] Weigh 40mg of magnetic nanoparticles Fe with a particle size of about 15nm 3 o 4 and 150 mg of Py-PEG were dissolved in 4 mL of n-hexane and 20 mL of deionize...

Embodiment 2

[0032](1) Preparation of Py-PEG:

[0033] 1mmol of 1-pyrenebutyric acid and 1mmol of polyethylene glycol monomethyl ether (MPEG) with a molecular weight of 5000, 40mL of dichloromethane, 3mmol of 1,3-dicyclohexylcarbodiimide (DCC), 1mmol of 4-bis Methylaminopyridine (DMAP) and 3 mmol triethylamine (TEA) were reacted at room temperature for 24 hours under the protection of nitrogen. After the reaction was completed, the by-product (dicyclohexyl urea) was removed by filtration, and the solvent was removed under reduced pressure and dissolved in dichloromethane. The reaction product is precipitated in excess ether or petroleum ether, and the product after filtration and purification is the polymer Py-PEG containing fluorescent functional groups;

[0034] (2) Preparation of magnetic fluorescent nanoparticles (FMNPs):

[0035] Weigh 50mg of magnetic nanoparticles MnFe with a particle size of about 13nm 2 o 4 and 200 mg of Py-PEG were dissolved in 4 mL of n-hexane and 20 mL of de...

Embodiment 3

[0037] (1) Preparation of Py-PEG:

[0038] 2mmol of 1-pyrenebutyric acid and 2mmol of polyethylene glycol monomethyl ether (MPEG) with a molecular weight of 5000, 70mL of tetrahydrofuran, 6mmol of 1,3-dicyclohexylcarbodiimide (DCC), 3mmol of 4-dimethylamino Pyridine (DMAP) and 6 mmol triethylamine (TEA) were reacted at room temperature for 40 hours under the protection of nitrogen. After the reaction was completed, the by-product (dicyclohexyl urea) was removed by filtration, and the solvent was removed under reduced pressure to dissolve the reaction product with dichloromethane , precipitated in excess ether or petroleum ether, and the product after filtration and purification is the polymer Py-PEG containing fluorescent functional groups;

[0039] (2) Preparation of magnetic fluorescent nanoparticles (FMNPs):

[0040] Weigh 60mg of magnetic nanoparticles Fe with a particle size of about 20nm 2 o 3 and 280 mg of Py-PEG were dissolved in 6 mL of n-hexane and 30 mL of deioni...

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Abstract

The invention discloses a magnetic fluorescent bifunctional nano biological probe and a preparation method thereof. The invention is characterized by preparing magnetic fluorescent bifunctional nanoparticles which can be used a nano biological probe, wherein each nanoparticle is in a core-shell spherical structure, and the nanoparticles are prepared from hydrophobic superparamagnetic nanoparticles as the core layer and a fluorescent amphipathic polymer as the shell layer by a nano emulsion process. After entering cells, the composite magnetic fluorescent nanoparticles can be used for imaging analysis on the cells respectively by a fluorescence process and a magnetic resonance imaging process. The magnetic fluorescent nano probe disclosed by the invention has high sensitivity and stability, greatly enhances the sensitivity and accuracy of cell imaging, reduces the emergence of false positive and false negative in cell diagnosis and analysis, and can be well used for diagnosing various diseases, such as tumor cells, cancer cells and the like.

Description

technical field [0001] The invention belongs to the field of macromolecular materials and medical engineering, and in particular relates to a magnetic-fluorescence dual-functional nano-biological probe and a preparation method thereof. Background technique [0002] Compared with conventional magnetic materials, magnetic nanoparticles and their composite particles have the following characteristics: (1) magnetic responsiveness, small particle size, large specific surface area, good suspension stability, and surface labeling; (2) associated with The characteristic physical length related to magnetism is just at the nanometer level, for example, the magnetic domain changes from multiple magnetic domains to single magnetic domains, making its magnetic properties 1000 times higher than the original; (3) lower than the superparamagnetic critical size presented Superparamagnetism can avoid magnetic agglomeration. Due to the above characteristics, the application of magnetic nanoma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12Q1/68G01N33/50G01N27/00A61B5/00B82Y15/00B82Y40/00
Inventor 任天斌李建波刘秋明黄超贾梦虹
Owner 苏州同科生物科技有限公司
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