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Grifola frondosa strain for producing polysaccharide with composite raw material of rice bran and wheat bran

A technology of Grifola frondosa and Grifola frondosa polysaccharides, applied in the direction of fungi, etc.

Inactive Publication Date: 2012-08-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a suitable strain of Grifola frondosa can be screened out, a breakthrough may be made in research and production

Method used

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  • Grifola frondosa strain for producing polysaccharide with composite raw material of rice bran and wheat bran
  • Grifola frondosa strain for producing polysaccharide with composite raw material of rice bran and wheat bran
  • Grifola frondosa strain for producing polysaccharide with composite raw material of rice bran and wheat bran

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Embodiment 1

[0047] Embodiment 1 Grifola frondosa ultraviolet-microwave compound mutagenesis

[0048] ① Preparation of spore suspension

[0049] Inoculate Grifola frondosa on a 9 cm sterilized solid medium plate and culture it for 4 days, then wash it with 10 mL of sterile saline, brush the vegetative body back and forth with a sterilized brush, and collect the washing solution.

[0050] ② Selection of ultraviolet mutagenic effect curve and ultraviolet mutagenic dose

[0051] Take 20 9 cm plates, each containing 5 mL of freshly prepared spore suspension. Each pair is divided into 10 groups. Irradiate for 0, 5, 10, 15, 20, 25, 30, 45, 60, and 150 s respectively, and then take 1 mL of each dish for appropriate dilution and spread on the plate. The plates were wrapped with black cloth and then cultured in a dark 28°C incubator with no light. Count the colonies after the plate grows out. For each group, the average number of colonies in two dishes was used as the number of colonies in t...

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Abstract

The invention discloses a Grifola frondosa strain for producing polysaccharide with a composite raw material of rice bran and wheat bran, which belongs to the technical field of microbial application technology and food biology. The Grifola frondosa strain JSU10 is preserved in China general microbiological culture collection center (CGMCC) in October 8th, 2010 with the CGMCC No. 4179 and is identified to be Grifolasp. The invention improves the positive mutation rate of the Grifola frondosa strain for rapidly growing and producing Grifola frondosa polysaccharide on a composite culture medium of rice bran and wheat bran by composite mutagenesis of ultraviolet rays and microwave to obtain a high-yield strain; the strain and the original strain are respectively a liquid fermentation rice bran and wheat bran composite culture medium; and hypha dry weight and polysaccharide of the mutated strain are respectively improved by 39.24 percent and 42.58 percent compared with the original strain.

Description

technical field [0001] The invention relates to the technical field of food microbe application, in particular to a grifola frondosa strain suitable for growing on rice bran and bran composite medium and high-yielding polysaccharide. Background technique [0002] Scientific research at home and abroad and the domestic market have shown that edible fungus polysaccharides can enhance human immunity and assist in the treatment of tumors and other diseases, and have become an auxiliary product for tumor biotherapy. Domestic medicinal products made from the fruiting bodies of edible fungi include Ganoderma lucidum capsules from Wuhu, Anhui, and Grifola frondosa capsules from Qingyuan, Zhejiang. Non-pharmaceutical health products made from edible fungus extracts are also sold. Internationally, New Zealand produces "GanoPoly" polysaccharide extract series products, which are sold to the United States, Australia, China (including Hong Kong and Taiwan) and other places. The inventor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 刘伟民张建郭春梅徐立平任晓锋顾慧敏杨锁华赵杰文
Owner JIANGSU UNIV
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