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Application of perylenequinone compound in preparation of feed for preventing and treating porcine viruses

A compound and pig feed technology, applied in animal feed, animal feed, applications, etc., can solve problems such as easy decomposition, hypericin cannot be effectively used, and hypericin’s chemical properties are unstable

Inactive Publication Date: 2011-06-29
江苏竹红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical properties of hypericin are unstable, and it is easy to decompose when exposed to light. In practical applications, a considerable part of hypericin cannot be effectively utilized.

Method used

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  • Application of perylenequinone compound in preparation of feed for preventing and treating porcine viruses
  • Application of perylenequinone compound in preparation of feed for preventing and treating porcine viruses
  • Application of perylenequinone compound in preparation of feed for preventing and treating porcine viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: In vitro antiviral experiment of perylenequinone compounds

[0044] (1) Preparation of cells for in vitro antiviral experiments

[0045] The effects of perylenequinone compounds on highly pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) were studied with Marc-145 cells (monkey kidney cells). Growth medium: DMEM (Dulbecco's modified eagle medium, containing 4500mg / L D-glucose, 300mg / L L-glutamine, 110mg / L sodium pyruvate, 5% fetal bovine serum, 1% penicillin, 1% streptavidin white). After digesting Marc-145 cells with 0.25% trypsin, the cells were dispersed by pipetting, and diluted to 1×10 with DMEM growth medium. 6 Cells / mL, add to 96-well cell culture plate, 37°C, 5% CO 2 Cultured in an incubator, after the cells grew into a single layer of cells, the in vitro antiviral test was carried out.

[0046] The effects of perylenequinone compounds on porcine parvovirus (PPV), porcine pseudorabies virus (PRV) and porcine circovirus (PCV) w...

Embodiment 2

[0074] Example 2: Anti-PRRSV virus experiment of solid-state fermented hypocrellin added to feed

[0075] The basic dietary formula of pigs in the experiment is: corn 65%, soybean meal 12%, secondary meal 15%, rapeseed cake 3%, fish meal 1.8%, NaCl 0.3%, CaCO 3 0.8%, CaHPO 4 0.8%, lysine 0.3%, multidimensional 1% (can provide vitamin A 1200IU per kg feed, vitamin D 32500IU, vitamin E 30IU, vitamin K 3mg, vitamin B 18μg, riboflavin 4mg, niacin 40mg, Pantothenic acid 15mg, choline chloride 400mg. Folic acid 700g, thiamine VB 1.5mg, vitamin B 3mg, biotin 100g, Zn 80mg, Mn 20mg, Fe 83mg, Cu 25mg). The basic diet is powder, and corn and soybean meal are all crushed with 1.8mm sieves.

[0076] Hypocretin is produced by solid-state fermentation, corn is the main raw material, and 2% glucose and 0.2% KH based on the weight of corn are added 2 PO 4 , 0.04% MgSO 4 ·7H 2 O. Add water and mix the material to make it wet, control the ratio of material to water at 1:0.7, sterilize at...

Embodiment 3

[0088] Example 3: Anti-PCV virus experiment of liquid fermented hypocrellin added to feed

[0089] The experimental basal diet was the same as in Example 2.

[0090] Hypocretin is produced by liquid fermentation method, and the culture medium per liter consists of: glucose 20g, peptone 50g, potato 200g, (NH 4 ) 2 HPO 4 2g, KH 2 PO 4 2g, MgSO 4 0.5g / L, sterilized at 121°C for 20min, then add 10% liquid bamboo yellow fungus seeds after cooling. Both the initial pH and the pH of the fermentation process are natural, the fermentation temperature is 30° C., and the fermentation ends in 72 hours. The fermented liquid was removed by centrifugation to obtain mycelium, which was dried at 60°C. It was determined that hypocrellin A accounted for 8.1%, hypocrellin B accounted for 1.3%, and the total amount of other perylenequinone compounds accounted for 0.2%.

[0091] 40 piglets (average body weight 6.75kg) in good body condition after weaning at 25 days were divided into 4 groups...

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Abstract

The invention discloses application of a perylenequinone compound in preparation of a feed for preventing and treating porcine viruses, and belongs to the technical fields of biological engineering and agriculture. The invention relates to the application of the perylenequinone compound added into a porcine feed to prepare the feed for preventing and treating porcine viruses such as porcine reproductive and respiratory syndrome virus, porcine circovirus, pseudorabies virus, porcine parvovirus, classical swine fever virus and the like. The perylenequinone compound comprises hypocrellin, elsinochrome, phleichrome, cladosporium, calphostin, cercosporin and the like; and the perylenequinone compound has a series of advantages of convenience in extraction, good stability, no obvious side effect in oral administration, quick metabolism and the like. The dosage in the feed for preventing the porcine viruses is 1 to 10mumol perylenequinone compound per kilogram weight every day, and the dosage in the feed for treating the porcine viruses is 10 to 100mumol perylenequinone compound per kilogram weight every day; the perylenequinone compound has very remarkable effect of preventing and treating the porcine viruses; and meanwhile, the perylenequinone compound can be produced by modern biological technology, and can produce good economic and social benefits.

Description

technical field [0001] The application of perylenequinone compounds in pig feed belongs to the field of bioengineering and agricultural technology. The invention relates to adding a perylenequinone compound to pig feed for preparing pig virus-resistant feed. Background technique [0002] With the continuous improvement of people's living standards, the consumption of pork is also increasing, which in turn drives the rapid development of the domestic breeding industry. However, frequent outbreaks of pig diseases have caused great losses to my country's pig industry, and pig farm biosafety systems have drawn much attention from pig producers. Pigs will be infected with a variety of viruses and bacteria during the feeding process, which will affect the immune function of pigs. Some diseases can lead to direct death of pigs, and some chronic diseases may cause slow growth and poor feed utilization although the mortality rate is not high. Reduced, complicated by secondary infec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/17A61K31/122A61P31/12A23K1/18A23K20/111A23K50/30
Inventor 蔡宇杰廖祥儒朱建航丁彦蕊张大兵
Owner 江苏竹红生物科技有限公司
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