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Preparation method of silk fibroin-poly(hydroxybutyrate-hydroxyvalerate) composite fiber membrane

A technology of polyhydroxybutyrate copolyester and composite fiber membrane, which is applied in the direction of cellulose/protein conjugated artificial filament, spinning solution preparation, fiber treatment, etc., to achieve good mechanical properties and breathable moisture permeability, The preparation method is simple and the effect of high porosity

Inactive Publication Date: 2011-06-22
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alone polyhydroxybutyrate copolyester has also been successfully electrospun into fibers, but so far, combining the respective advantages of silk fibroin and polyhydroxybutyrate copolyester, and having good mechanical properties and Biocompatible silk fibroin and polyhydroxybutyrate copolyester composite fiber membrane has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Preparation of silk fibroin: Put the silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove the sericin on the surface of the silkworm cocoons, take them out, squeeze them dry, wash them with water, and dry them at 60°C to prepare silk For silk fiber, a mixed solution of dry silk fiber and calcium chloride, ethanol and water with a molar ratio of 1:8:2 is dissolved at 75°C to form a silk fibroin solution, and ethanol and chlorine in the solution are removed by dialysis Calcium, filtered to obtain silk fibroin solution, and then freeze-dried at -80°C to obtain silk fibroin;

[0021] b. Dissolve silk fibroin in hexafluoroisopropanol, stir until completely dissolved, and obtain a silk fibroin solution with a mass fraction of 5%;

[0022] c. Dissolve polyhydroxybutyrate valerate copolyester in hexafluoroisopropanol, stir until completely dissolved to obtain a polyhydroxybutyrate valerate copo...

Embodiment 2

[0028] a. Preparation of silk fibroin: Put the silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove the sericin on the surface of the silkworm cocoons, take them out, squeeze them dry, wash them with water, and dry them at 60°C to prepare silk For silk fiber, a mixed solution of dry silk fiber and calcium chloride, ethanol and water with a molar ratio of 1:8:2 is dissolved at 75°C to form a silk fibroin solution, and ethanol and chlorine in the solution are removed by dialysis Calcium, filtered to obtain silk fibroin solution, and then freeze-dried at -80°C to obtain silk fibroin;

[0029] b. Dissolve silk fibroin in trifluoroethanol, stir until completely dissolved, and obtain a silk fibroin solution with a mass fraction of 15%;

[0030] c. Dissolve polyhydroxybutyrate valeric acid copolyester in trifluoroethanol, stir until completely dissolved, and obtain a polyhydroxybutyrate valeric acid cop...

Embodiment 3

[0036] a. Preparation of silk fibroin: Put the silkworm cocoons into a sodium carbonate solution with a mass percentage concentration of 0.5% and stir, boil for 60 minutes to remove the sericin on the surface of the silkworm cocoons, take them out, squeeze them dry, wash them with water, and dry them at 60°C to prepare silk For silk fiber, a mixed solution of dry silk fiber and calcium chloride, ethanol and water with a molar ratio of 1:8:2 is dissolved at 75°C to form a silk fibroin solution, and ethanol and chlorine in the solution are removed by dialysis Calcium, filtered to obtain silk fibroin solution, and then freeze-dried at -80°C to obtain silk fibroin;

[0037] b. Dissolve silk fibroin in hexafluoroacetone, stir until completely dissolved, and obtain a silk fibroin solution with a mass fraction of 8%;

[0038] c. Dissolve polyhydroxybutyrate valerate copolyester in trifluoroethanol and stir until completely dissolved to obtain a polyhydroxybutyrate valerate copolyeste...

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Abstract

The invention discloses a preparation method of a silk fibroin-poly(hydroxybutyrate-hydroxyvalerate) composite fiber membrane, which comprises the steps of: mixing a prepared silk fibroin solution with a poly(hydroxybutyrate-hydroxyvalerate) solution to prepare a spinning solution, and conducting electrostatic spinning and aftertreatment. Based on the electrostatic spinning method, the composite fiber membrane is prepared, and the fiber membrane has good biocompatibility of the silk fibroin and the excellent mechanical performance of the poly(hydroxybutyrate-hydroxyvalerate) simultaneously, is more applicable to the biomedical field, and is expected to be used as a material for engineering of skin, nerves and cartilaginous tissues. The prepared composite fiber membrane has higher porosity, good mechanical performance, good air permeability and moisture permeability and large liquid absorption amount, can be degraded in human body and can promote tissue repair; and the preparation method has simple process.

Description

technical field [0001] The invention relates to a preparation method of a fiber membrane, in particular to a preparation method of a composite fiber membrane of silk fibroin and polyhydroxybutyrate copolyester. Background technique [0002] Tissue engineering is to plant in vitro cultured and expanded cells on a biomaterial with good biocompatibility and a certain spatial structure, culture them in vitro to multiply the cells, and then transplant them to the desired site together. Cells continue to proliferate, while the biological scaffold structure is gradually degraded and absorbed, resulting in the formation of new tissues and organs, thereby achieving the purpose of tissue repair. Therefore, selecting appropriate biomaterials and preparation methods to construct tissue engineering scaffolds is the basis of tissue engineering research. Electrospinning is a process that uses a polymer solution or melt to form a jet stream under the action of a strong electric field for s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H3/00D01D5/00D01D1/02D01F8/02D01F8/14D04H1/4382D04H1/728
Inventor 朱海霖陈建勇冯新星吴斌伟
Owner ZHEJIANG SCI-TECH UNIV
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