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Method for quickly identifying resistance to banded sclerotial blight in rice seedling stage

A technology for sheath blight and rice seedlings, applied in the field of rapid identification of rice sheath blight resistance at the seedling stage, can solve the problems of large environmental impact, long time-consuming identification methods, heavy workload, etc., and achieve low labor intensity and short identification period , The effect of fast and effective screening

Active Publication Date: 2011-06-08
HUAWEI TEHCHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for quickly identifying rice sheath blight resistance at the seedling stage in view of the current situation that the identification method of sheath blight resistance is greatly affected by the environment, and the existing identification method takes a long time and has a large workload

Method used

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  • Method for quickly identifying resistance to banded sclerotial blight in rice seedling stage
  • Method for quickly identifying resistance to banded sclerotial blight in rice seedling stage
  • Method for quickly identifying resistance to banded sclerotial blight in rice seedling stage

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Embodiment 1

[0017] The rice sheath blight pathogen taken in this embodiment: the strain of sheath blight (the hyphae fusion group of Rhizoctonia solani is AG1-IA) is derived from the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, and is a strong pathogenic strain, numbered for RH-9.

[0018] (1) Strain activation: crush the sclerotia of the strain RH-9 on the ultra-clean workbench, sprinkle it evenly on the surface of the PDA medium, place it in the dark at about 28°C for about 40 hours, and pick the colony with an inoculation loop Border bacteria, placed on the surface of a new PDA medium (sterile operation), cultivated at 25-30°C for about 36 hours, until the surface of the PDA medium is covered with hyphae.

[0019] (2) Preparation of the inoculum: get the mycelium piece with a puncher with a diameter of 1-1.5cm on the new PDA medium of step (1), place it on another PDA medium (by 64cm 2 Place two above-mentioned hyphae pieces on the surface of the PDA medium...

Embodiment 2

[0031] Identified varieties: Lemont, Wuyujing 3, Nipponbare, Wuyunjing 19, Teqing, Yanyou 1. These varieties were identified for resistance to rice sheath blight at the seedling stage, and all varieties were owned by the laboratory or purchased from the market. The identification results are shown in Table 3.

[0032] Table 36 Types of rice variety resistance to sheath blight at seedling stage

[0033]

[0034] All the other implementations are as in Example 1.

Embodiment 3

[0036]Lemont, Wuyujing 3, Nipponbare, Teqing, Wuyunjing 19, and Yanyou 1 were identified for rice sheath blight resistance at the adult plant stage. After aseptic treatment, inoculate and culture on PDB liquid medium in a petri dish for 3 to 5 days, and use it for inoculation when the mycelium is densely covered with the petri dish. At the end of rice tillering, use tweezers to insert a toothpick into the inner side of the three-leaf leaf sheath . The disease grade was investigated 30 days after heading, and the disease grade standards were as follows: the plants of grade 0 were disease-free; On the inverted 4 leaf sheath; at grade 4, the lesion is on the inverted 3 leaf sheath; at grade 5, the lesion height is on the inverted 2 leaf sheath; at grade 6, the lesion height is within half the length of the exposed part of the sword sheath; at grade 7, the entire sword sheath Injured; Lesions appear on the flag leaf leaves at grade 8; Plants die abnormally at grade 9. The resist...

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Abstract

The invention discloses a method for quickly identifying resistance to banded sclerotial blight in rice seedling stage, comprising the following steps: activating and culturing the strain of rhizoctonia solani so as to obtain mycelium; overgrowing the mycelium on strip medical absorbent cottons through a culture medium so as to form absorbent cottons with bacteria and good moisturizing performance; soaking seeds of the rice variety (series) to be identified to accelerate germination at the temperate of 30 DEG C after disinfecting, and then seeding in the soil inside the box body, and culturing for 3-4 leaf ages; intertwining the straw base of the seedling with the strip absorbent cotton with rhizoctorzia solani, then covering a plastic cover (taken off 24 hours later) on the seedlings, and carrying out normal growth management of the seedlings; and identifying the resistance to banded sclerotial blight in the rice seedling stage according to seedling-straw ratio 5-7 days after inoculation.

Description

Technical field: [0001] The invention belongs to the technical field of rice disease prevention and control, and in particular relates to a method for quickly identifying rice sheath blight resistance at seedling stage. Background technique: [0002] The pathogen of rice sheath blight is Rhizoctonia solani Kühn in anamorphic form and Thanatephorus cucumeris (Frank) Donk in asexual form, which mainly harms plants in anamorphic form. Since Chujiro Sasaki first discovered rice sheath blight in 1905, it has a history of more than 100 years. Sheath blight mainly affects leaf sheaths, but can also infect leaves, stems, and ears. It can damage rice throughout its life, but usually there are 2 main damage periods. One is from the rice tillering stage to the early jointing stage, and the other is from booting to 15 days after heading. At this time, the rice population is in a false closure or closure period, and the humidity in the field is high, which is conducive to the disease,...

Claims

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Application Information

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IPC IPC(8): A01G16/00A01G7/06C12N1/14A01G22/22
Inventor 张国良
Owner HUAWEI TEHCHNOLOGIES CO LTD
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