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Pseudomonad DA10 bacterial strain and obtaining method and application thereof

A technology for pseudomonas and strains, which is applied to pseudomonas strains and their application fields, and can solve problems such as failure to realize industrialization.

Inactive Publication Date: 2012-11-07
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the application of microorganisms and the enzymes they produce in flax degumming can not only improve the yield and quality of fibers, consume less energy, be safe to use, and reduce environmental pollution, it has a good prospect, but its industrialization has not been realized.

Method used

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  • Pseudomonad DA10 bacterial strain and obtaining method and application thereof
  • Pseudomonad DA10 bacterial strain and obtaining method and application thereof
  • Pseudomonad DA10 bacterial strain and obtaining method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Screening and Obtaining of Flax Degumming Strains

[0059] (1) Sampling rotting seaweed from the Zhoushan sea area of ​​the East China Sea, first put the seaweed and the enrichment medium at a ratio of 1g: 20ml in the enrichment medium and culture it at room temperature for 30 days, then take 0.1ml of the enrichment medium and apply it to the separation medium Culture medium, static culture at 37°C for 1 to 4 days, obtained from the wild-type degumming dominant strain;

[0060] Among them, the formula of the enrichment medium is: 5 g of flax powder, 200 ml of sterile water, and the pH is natural; the formula of the separation medium is: 5 g of flax powder, (NH 4 ) 2 SO 4 5g,K 2 HPO 4 1g, MgSO 4 0.5g, KCl 0.5g, FeSO 4 0.01, 20g agar, 1000mL water, adjust the pH to 9 with NaOH;

[0061] (2) Inoculate 4 loops of the bacterial strain obtained in step (1) into pectin nutrient medium (petri dish diameter 10cm), cultivate at 28°C for 72h, after the formation of colo...

Embodiment 2

[0080] In order to enable the strain to maintain a stable high-yielding characteristic, beef extract peptone medium is used as the slant preservation medium. The raw materials and preparation methods of the medium are: 0.5g beef extract, 1g peptone, and 0.5g sodium chloride (pH7.4-7.6) , add water to 100ml constant volume, culture overnight at 37°C 200rpm, add 100ml of freezing buffer; among them, the freezing buffer consists of 0.0627g of potassium dihydrogen phosphate, 0.0177g of dipotassium hydrogen phosphate, 0.0588g of sodium citrate, sulfuric acid heptahydrate Magnesium 0.02645g, glycerin 10ml, add water to volume to 100ml; divide nutrient broth medium (beef extract 0.3g, peptone 1g, sodium chloride 0.5g, add water to 100ml volume, pH7.4-7.6) In 250mL containers, 50mL in each container, after sealing and bandaging, sterilize at 121°C for 20min; after cooling, insert the bacteria loop cultured on a slant for 24h, and shake the flask at a speed of 200r / min and a temperature...

Embodiment 3

[0090] The flax degumming process is as follows:

[0091] Put 5g of the original flax stem into a 250ml conical flask, divide the flax flax and the fermented bacteria liquid into the conical flask at a bath ratio of 1:20, the liquid volume is 100ml, degumming in a shaker at 40°C and 200rpm for 1 day , 2 days and 4 days; when the degumming time is up, remove the degumming solution, boil in a boiling water bath for 5 minutes, rinse with tap water several times to remove bacteria on the linen, and stop degumming.

[0092] Through the observation of degummed flax, it was found that the flax fibers were obviously dispersed after 1 day, and the color of the fermentation broth became dark and turbid; after 2 days of degumming, the original bundled flax was completely dispersed into fibers, and the fermentation broth was obviously thickened; after 4 days of degumming, the degumming rate of flax reached more than 90 percent.

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Abstract

The invention relates to a pseudomonad DA10 bacterial strain and an obtaining method and application thereof. The bacterial strain has the capability of producing pectinase and hemicellulase and has the flax degumming activity. The bacterial strain and a culture system can be directly used for flax degumming, the bacterial strain has the advantages of short degumming period, low treatment cost, good fiber quality, mild degumming condition, strong anti-pollution capability, good heat resistance performance, no environment pollution and the like, the fiber dispersion rate reaches 100 percent, the degumming efficiency reaches 95 percent, and the bacterial strain can not be polluted. Compared with the prior art, the bacterial strain has the characteristic of simple process, is applied to large-scale industrial production and has potential theoretic and practical significance on the flax degumming of the pseudomonad bacterial strain under the alkaline condition.

Description

technical field [0001] The invention belongs to the field of Pseudomonas strains and applications thereof, in particular to a Pseudomonas DA10 strain and its obtaining method and application. Background technique [0002] Flax fiber is natural and unsophisticated, with soft colors and unique cool comfort, which is not available in chemical fibers and incomparable to other natural fibers. It is known as "the queen of plant fibers". Linen products have smooth hand feeling, natural texture, soft color, strong and durable styles, and have natural functions such as anti-virus and anti-bacterial, anti-ultraviolet, low static electricity, low magnetic field effect, etc. It is unique in style and function, and has the characteristics of "green health care" , so it is favored by consumers and is one of the most popular textile products in the international market. [0003] At present, traditional flax warm water retting, rain dew retting and natural water retting methods are commonl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20D01C1/04D06M16/00C12S11/00C12R1/38D06M101/06
Inventor 张兴群丁若垚郁崇文董政娥
Owner DONGHUA UNIV
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