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Hsp70 gene molecular marker identifying goose stress resistance and application thereof

A technology of molecular markers and genes, applied in the field of genetic engineering, achieves the effect of low price, simple conditions and high reliability

Inactive Publication Date: 2011-01-12
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there are relatively few studies on the molecular markers of goose stress resistance, and the heat resistance molecular markers found in the coding region of goose Hsp70 in the present invention are reported for the first time

Method used

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  • Hsp70 gene molecular marker identifying goose stress resistance and application thereof
  • Hsp70 gene molecular marker identifying goose stress resistance and application thereof
  • Hsp70 gene molecular marker identifying goose stress resistance and application thereof

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Embodiment

[0029] The blood of 200 black-bone geese was collected from the subwing vein at the ambient temperature of 20°C and 35°C respectively, and divided into three tubes: 1-2 mL of non-anticoagulated blood, serum for T3 and corticosterone determination; 1-2 mL of anti-coagulated blood , for the determination of H / L; anticoagulant blood 1-2ml, for the extraction of genomic DNA. Among them, non-anticoagulated blood was directly centrifuged to absorb serum, and T3 and corticosterone were measured by enzyme-linked immunosorbent assay; anticoagulated blood was used to measure H / L, and the blood smear was stained by Wright’s and Giemsa’s composite staining method, and microscopic examination was performed. , according to Alfred's white blood cell classification method for cell classification, and according to Campo's method for counting and calculation; CD3 + , CD4 + Detection by flow cytometry.

[0030] Extract the genomic DNA of 400 blood samples, take 10ul for each sample, and make D...

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Abstract

The invention discloses an Hsp70 gene molecular marker identifying the goose stress resistance and application thereof. The Hsp70 gene molecular marker identifying the goose stress resistance is T+1122C, and is positioned in a coding region of a heat stress protein Hsp70. PCR amplification is performed on the coding region of the Hsp70 by constructed DNA serving as a template; the single nucleotide polymorphism sites SNPs of the amplified product are screened by a direct PCR sequencing method; the screened SNPs are subjected to genotying by applying a PCR-RFLP method in an experimental goose group; and the genotyping result and the character index of the group are subjected to correlation analysis, and a remarkably related SNP site is the molecular marker. Results show that the TC genotype individual has high stress resistance.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to an Hsp70 gene molecular marker for identifying stress resistance of geese and an application thereof. Background technique [0002] Molecular marker-assisted selective breeding is an important application of genetic engineering in modern poultry breeding. On-site breeding through molecular markers can not only shorten the breeding time limit, greatly reduce the human and material resource consumption of breeding, but also increase the diversity of molecular markers. The application potential of auxiliary molecular markers in field breeding is greatly improved. The intensive development of modern poultry breeding industry makes poultry face more and more external stresses, while geese are more susceptible to stress due to the increase in breeding density and the limited breeding water area. Therefore, improving the stress resistance of individuals through breeding is one of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 罗庆斌肖雄甘建伉张细权聂庆华林果
Owner SOUTH CHINA AGRI UNIV
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