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Method for detecting diethylstilbestrol and special chemiluminescent immunoassay kit thereof

A technique of chemiluminescence immunity and diethylstilbestrol is applied in the field of detection of diethylstilbestrol, which can solve the problems of great harm to human and animal health, residues, harm to public health and the like, and achieves the effects of low cost, simple operation, good preservation and stability.

Active Publication Date: 2011-01-05
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many scientific experiments have confirmed that diethylstilbestrol is a carcinogen, and has serious side effects, mainly reproductive toxicity, which is harmful to human and animal health. Now many countries such as Europe, America and China have banned its use in animal feed , but it is often used illegally in livestock production, resulting in residues in livestock and poultry products, endangering public health

Method used

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  • Method for detecting diethylstilbestrol and special chemiluminescent immunoassay kit thereof
  • Method for detecting diethylstilbestrol and special chemiluminescent immunoassay kit thereof
  • Method for detecting diethylstilbestrol and special chemiluminescent immunoassay kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, preparation and detection method of chemiluminescent immunoassay kit

[0040] 1. The composition of the chemiluminescence immunoassay kit:

[0041] (1) Coating original solution: obtained by dissolving the coating original in the coating buffer, wherein the concentration of the coating original in the coating original solution is 0.08 μg / mL; the coating original is diethylstilbestrol hapten and egg white protein conjugates.

[0042] (2) Horseradish peroxidase-labeled goat anti-mouse anti-antibody working solution:

[0043] It is obtained by diluting horseradish peroxidase-labeled goat anti-mouse anti-antibody with diluent, and the dilution ratio is 1:500;

[0044] The diluent is obtained by mixing 50mL bovine serum albumin and 950mL phosphate buffer; the concentration of the phosphate buffer is 0.02M, and the pH value is 7.4.

[0045]Horseradish peroxidase-labeled goat anti-mouse anti-antibody was purchased from Jackson ImmunoResearch Laboratories Inc. ...

Embodiment 2

[0096] Embodiment 2, test kit sensitivity, accuracy and shelf life test

[0097] 1. Kit sensitivity experiment

[0098] The zero standard solution (pH7.4, 0.05M phosphate buffer) was tested 20 times, and the mean value of the measurement results plus 3 times the standard deviation was used as the minimum detection limit of the kit.

[0099] Table 1 Statistical table of zero standard measurement results μg / L

[0100]

[0101] It can be seen from Table 1 that the minimum detection limit of the kit is 0.5 μg / L.

[0102] 2. Standard product precision test:

[0103] From the three batches of kits described in Example 1 (batch 01, batch 02, and batch 03), 10 kits were extracted from each batch, and the luminous intensity value of the 5 μg / L diethylstilbestrol standard solution was measured to calculate the coefficient of variation. The detection method is consistent with that described in Experiment 3 in Example 1.

[0104] The experiment was repeated three times, and the res...

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Abstract

The invention discloses a method for detecting diethylstilbestrol and a special chemiluminescent immunoassay kit thereof. The chemiluminescent immunoassay kit for detecting diethylstilbestrol comprises a diethylstilbestrol specific antibody, a coating antigen and a standard solution, wherein the coating antigen is a conjugate of diethylstilbestrol hapten and carrier protein, and the constitutional formula of the coating antigen is as shown in a formula I. The detection method has the characteristics of simple preprocessing of samples, easy operation, low cost, high specificity, high sensitivity, high accuracy, and the like, and is capable for on-site monitoring and suitable for screening a large amount of samples.

Description

technical field [0001] The invention relates to a method for detecting diethylstilbestrol and a special chemiluminescent immunoassay kit thereof. Background technique [0002] At present, the safety of animal food has become the focus of attention all over the world, and the problem of veterinary drug residues is one of the most important factors affecting the safety of animal food. Due to the complex composition of animal samples, the concentration of analytes is low, and most of the samples are very small , which puts forward higher requirements on the selectivity and sensitivity of the analytical method. Chemiluminescence immunoassay (Chemiluminescence analysis, CLIA) technology combines highly sensitive chemiluminescence technology with highly specific immune reaction, which has high sensitivity, strong specificity, wide linear range, easy operation, and does not require very expensive equipment Features. CLIA does not require external light sources, has a higher signa...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/577G01N33/531C07K16/44C12N5/20C12R1/91
Inventor 王战辉沈建忠张素霞史为民吴聪明程林丽曹兴元汤树生
Owner CHINA AGRI UNIV
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