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Bacterial strain tolerant with various inhibitors of Saccharomyces cerevisiae

A technology of Saccharomyces cerevisiae and inhibitor, applied in the field of Saccharomyces cerevisiae, can solve the problems affecting the ethanol fermentation yield and rate of cellulose hydrolyzate, and achieve the effect of high-efficiency ethanol production

Active Publication Date: 2010-11-10
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since these chemical pretreatment conditions are generally high temperature and high pressure, it will lead to the production of some inhibitory substances, such as furans, weak acids, phenols, etc.
These inhibitory substances have a very strong inhibitory effect on subsequent fermentation microorganisms, seriously affecting the yield and rate of ethanol fermentation of cellulose hydrolyzate

Method used

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  • Bacterial strain tolerant with various inhibitors of Saccharomyces cerevisiae
  • Bacterial strain tolerant with various inhibitors of Saccharomyces cerevisiae
  • Bacterial strain tolerant with various inhibitors of Saccharomyces cerevisiae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation of YYJ003 strain

[0023] A novel strain YYJ003 of Saccharomyces cerevisiae tolerant to various inhibitors of the present invention is obtained by carrying out ultraviolet mutagenesis to industrial Saccharomyces cerevisiae through tolerance and domestication of various inhibitors. The ultraviolet mutagenesis strategy is: the culture solution of the original S strain (YEPD medium: glucose 20g / L, yeast powder 10g / L, peptone 20g / L) is diluted and spread on the screening medium containing inhibitors (adding inhibitors YEPD medium), irradiate the paved plate at a distance of 30cm from a 15W ultraviolet lamp for 60 seconds, and place it in a 30°C incubator for 3 days in the dark. The strain acclimation step is as follows: the colonies grown on the selection medium are transferred to the liquid selection selection medium containing inhibitors for cultivation, and the cultivation conditions are 30° C., 180 rpm. When cultured to the stationary phase, according to OD ...

Embodiment 2

[0025] Comparison of the growth of the YYJ003 strain with the original strain on shake flasks

[0026] 1. Test materials: The strain YYJ003 was screened by our laboratory and preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, with the preservation number CGMCC NO.2757; the original strain Saccharomyces cerevisiae was purchased from Hubei Angel Yeast Co., Ltd., referred to as S strain.

[0027] 2. Test method:

[0028] Seed medium:

[0029] YYJ003 strain: glucose 20g / L, yeast powder 10g / L, peptone 20g / L, furfural 1.3g / L, phenol 0.5g / L, acetic acid 5.3g / L, sterilized at 121°C for 20min. Original strain: glucose 20g / L, yeast powder 10g / L, peptone 20g / L, sterilized at 121°C for 20min.

[0030] Fermentation medium:

[0031] Glucose 20g / L, yeast powder 10g / L, peptone 20g / L, sterilized at 121°C for 20min. Before fermentation, inhibitors were added to make the final concentration of furfural 1.3g / L, phenol 0.5g / L, and ace...

Embodiment 3

[0038] Comparing the fermentation performance of YYJ003 strain and the original strain in 2% glucose medium fermenter

[0039] 1. Test material: same as Example 2.

[0040] 2. Test method:

[0041] The preparation of seeds and fermentation medium is the same as in Example 2.

[0042] The YYJ003 strain and the original strain were inoculated in 100mL seed medium respectively, and cultured at 30°C and 180rpm for 12-14h, both at the initial cell concentration OD 600 =0.1 was inoculated in a 5L fermentation tube with 3L fermentation medium, cultured at 30°C and 300rpm, and the growth curve, glucose concentration, ethanol concentration and furfural concentration were measured.

[0043] 3. Analysis method:

[0044] The cell concentration was characterized by measuring the absorbance value at 600 nm with a 722-type spectrophotometer. Glucose concentration and ethanol concentration were measured by high performance liquid chromatography (Waters1515), the chromatographic column was...

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Abstract

The invention discloses a bacterial strain tolerant with various inhibitors of Saccharomyces cerevisiae, which is named as Saccharomyces cerevisiae YYJ003 and is collected in China Committee for Culture Collection of Microorganisms (CCCCM) with the collection number of CGMCC NO.2757. The bacterial strain tolerant with the various inhibitors of the Saccharomyces cerevisiae in the invention can be in normal growth in a culture medium containing inhibitors produced by diluted acid pretreatment and can realize efficient ethanol production. The bacterial strain in the invention breaks through the bottleneck problem that a dilute acid pretreatment method is limited to produce cellulosic ethanol.

Description

technical field [0001] The present invention relates to a brewer's yeast, in particular to a strain of brewer's yeast resistant to multiple inhibitors. Background technique [0002] In the cellulosic ethanol production process, acid pretreatment is favored by most institutions due to its low cost and strong operability, and is considered to be the easiest pretreatment method for industrialization, such as dilute acid pretreatment and steam explosion pretreatment . However, since these chemical pretreatment conditions are generally high temperature and high pressure, it will lead to the production of some inhibitory substances, such as furans, weak acids, phenols and so on. These inhibitory substances have a very strong inhibitory effect on subsequent fermentation microorganisms, seriously affecting the yield and rate of ethanol fermentation of cellulose hydrolyzate. Experiments have shown that these inhibitors inhibit a variety of fermenting microorganisms, including Esche...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12N13/00C12N15/01C12P7/06C12R1/865
CPCY02E50/17Y02E50/10
Inventor 元英进李炳志白云海查健王昕
Owner TIANJIN UNIV
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